##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       AclI        AcyI        AflII       AhdI        
Aor51HI     AscI        AseI        AsnI        AspEI       
AviII       BbeI        BbsI        BfrI        BpuAI       
BsaBI       BsaHI       BsiWI       BsmBI       BspDI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     ClaI        DraIII      Eam1105I    
Eco47III    EcoO65I     FseI        FspI        Hin1I       
HpaI        KasI        MamI        MluI        NarI        
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PmeI        PshAI       PshBI       Psp1406I    
PvuI        SacI        SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SwaI        
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

Acc65I       3507  GGTACC               G^GTACC
AgeI         3948  ACCGGT               A^CCGGT
Asp718I      3507  GGTACC               G^GTACC
AspI         1063  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1817  GGATCC               G^GATCC
BbvCI        4352  GCTGAGG              CCTCAGC(-5/-2)
BciVI        3874  GGATAC               GTATCC(6/5)
BclI         1322  TGATCA               T^GATCA
BglI         3384  GCCATGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3767  AGATCT               A^GATCT
BlpI          214  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      214  GCTGAGC              GC^T[ACGT]AGC
BsiEI        1272  CGGCCG               CG[GA][CT]^CG
BsrDI        3358  GCAATG               GCAATG(2/0)
CelII         214  GCTGAGC              GC^T[ACGT]AGC
CpoI          409  CGGACCG              CG^G[AT]CCG
DraI         4102  TTTAAA               TTT^AAA
EagI         1272  CGGCCG               C^GGCCG
EclXI        1272  CGGCCG               C^GGCCG
Eco52I       1272  CGGCCG               C^GGCCG
EcoNI         474  CCTGATACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        2905  GATATC               GAT^ATC
FbaI         1322  TGATCA               T^GATCA
HaeII        1277  GGCGCT               [GA]GCGC^[CT]
HincII       1069  GTCAAC               GT[CT]^[GA]AC
HindII       1069  GTCAAC               GT[CT]^[GA]AC
KpnI         3507  GGTACC               GGTAC^C
KspI          423  CCGCGG               CCGC^GG
MfeI         3269  CAATTG               C^AATTG
MunI         3269  CAATTG               C^AATTG
NdeI         4759  CATATG               CA^TATG
PflFI        1063  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3948  ACCGGT               A^CCGGT
PstI          385  CTGCAG               CTGCA^G
RsrII         409  CGGACCG              CG^G[AT]CCG
SacII         423  CCGCGG               CCGC^GG
SphI          795  GCATGC               GCATG^C
SspI         4516  AATATT               AAT^ATT
Tth111I      1063  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccIII        962  TCCGGA               T^CCGGA
             1337  TCCGGA              
Alw44I       1141  GTGCAC               G^TGCAC
             2167  GTGCAC              
ApaLI        1141  GTGCAC               G^TGCAC
             2167  GTGCAC              
AvaI           73  CCCGAG               C^[CT]CG[GA]G
              507  CTCGGG              
AvrII        2523  CCTAGG               C^CTAGG
             2774  CCTAGG              
BcgI         2682  CGATCCGGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              684  GCAAGACACTCG        
BlnI         2523  CCTAGG               C^CTAGG
             2774  CCTAGG              
BseAI         962  TCCGGA               T^CCGGA
             1337  TCCGGA              
BsgI         2081  CTGCAC               GTGCAG(16/14)
             3590  GTGCAG              
BsoBI          73  CCCGAG               C^[CT]CG[GA]G
              507  CTCGGG              
Bsp1407I      697  TGTACA               T^GTACA
             2366  TGTACA              
BspEI         962  TCCGGA               T^CCGGA
             1337  TCCGGA              
BspLU11I     4037  ACATGT               A^CATGT
             4794  ACATGT              
BsrBI         217  GAGCGG               CCGCTC(-3/-3)
             1222  GAGCGG              
BsrGI         697  TGTACA               T^GTACA
             2366  TGTACA              
BssSI         910  CACGAG               CACGAG(-5/-1)
             1083  CTCGTG              
DrdI         1860  GACTTGCTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2519  GACCCCTAGGTC        
EcoT22I      3937  ATGCAT               ATGCA^T
             4283  ATGCAT              
MroI          962  TCCGGA               T^CCGGA
             1337  TCCGGA              
NaeI          235  GCCGGC               GCC^GGC
             1274  GCCGGC              
NgoMIV        235  GCCGGC               G^CCGGC
             1274  GCCGGC              
NsiI         3937  ATGCAT               ATGCA^T
             4283  ATGCAT              
SapI         1044  GAAGAGC              GCTCTTC(1/4)
             3700  GAAGAGC             
SspBI         697  TGTACA               T^GTACA
             2366  TGTACA              
StuI         1409  AGGCCT               AGG^CCT
             2771  AGGCCT              
TaqI         1879  TCGA                 T^CGA
             2188  TCGA                
TthHB8I      1879  TCGA                 T^CGA
             2188  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApaI        ApoI        Asp700I     AspHI       AvaII       
BalI        BanI        BanII       BbrPI       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaAI       
BsaI        BsaJI       BsaWI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BspHI       BspMI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PmaCI       PmlI        PpuMI       PvuII       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Van91I      
XcmI        XhoII       XmnI