##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AclI AflII AgeI Aor51HI
AscI AseI AsnI AspI BbrPI
BfrI BsaAI BsaBI BsiWI Bsp1407I
BspDI BspHI BsrGI BssHII BstBI
ClaI CpoI DraI DrdI Eco47III
EcoRI EcoRV FseI HpaI MaeII
MamI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfuI SgrAI
SnaBI SphI SspBI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 3636 GGTACC G^GTACC
AcsI 2260 AAATTC [GA]^AATT[CT]
AhdI 2717 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2260 AAATTC [GA]^AATT[CT]
Asp700I 383 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3636 GGTACC G^GTACC
AspEI 2717 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 2395 GTATCC GTATCC(6/5)
BsiEI 1695 CGGCCG CG[GA][CT]^CG
Bst1107I 3819 GTATAC GTA^TAC
BstEII 929 GGTGACC G^GT[ACGT]ACC
BstPI 929 GGTGACC G^GT[ACGT]ACC
BstZ17I 3819 GTATAC GTA^TAC
EagI 1695 CGGCCG C^GGCCG
Eam1105I 2717 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1695 CGGCCG C^GGCCG
Eco52I 1695 CGGCCG C^GGCCG
EcoO65I 929 GGTGACC G^GT[ACGT]ACC
EcoT22I 2938 ATGCAT ATGCA^T
HindIII 2531 AAGCTT A^AGCTT
KpnI 3636 GGTACC GGTAC^C
KspI 93 CCGCGG CCGC^GG
NsiI 2938 ATGCAT ATGCA^T
SacII 93 CCGCGG CCGC^GG
SfiI 497 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 4356 ACTAGT A^CTAGT
SspI 5585 AATATT AAT^ATT
XmnI 383 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3620 GTCTAC GT^[AC][GT]AC
3819 GTATAC
AccIII 145 TCCGGA T^CCGGA
1040 TCCGGA
AflIII 1546 ACATGT A^C[GA][CT]GT
4914 ACATGT
AviII 1006 TGCGCA TGC^GCA
1285 TGCGCA
AvrII 1852 CCTAGG C^CTAGG
3527 CCTAGG
BamHI 1778 GGATCC G^GATCC
4000 GGATCC
BbeI 1000 GGCGCC GGCGC^C
2957 GGCGCC
BcgI 1301 GCAGGCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3609 GCAGGCCAGTCG
BclI 3748 TGATCA T^GATCA
4093 TGATCA
BglII 1198 AGATCT A^GATCT
1636 AGATCT
BlnI 1852 CCTAGG C^CTAGG
3527 CCTAGG
BsaWI 145 TCCGGA [AT]^CCGG[AT]
1040 TCCGGA
BseAI 145 TCCGGA T^CCGGA
1040 TCCGGA
BspEI 145 TCCGGA T^CCGGA
1040 TCCGGA
BspLU11I 1546 ACATGT A^CATGT
4914 ACATGT
BsrFI 1697 GCCGGC [GA]^CCGG[CT]
4051 GCCGGC
Cfr10I 1697 GCCGGC [GA]^CCGG[CT]
4051 GCCGGC
DraIII 924 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4290 CACTCAGTG
FbaI 3748 TGATCA T^GATCA
4093 TGATCA
FspI 1006 TGCGCA TGC^GCA
1285 TGCGCA
HincII 2106 GTTGAC GT[CT]^[GA]AC
2334 GTCAAC
HindII 2106 GTTGAC GT[CT]^[GA]AC
2334 GTCAAC
KasI 1000 GGCGCC G^GCGCC
2957 GGCGCC
MroI 145 TCCGGA T^CCGGA
1040 TCCGGA
NaeI 1697 GCCGGC GCC^GGC
4051 GCCGGC
NarI 1000 GGCGCC GG^CGCC
2957 GGCGCC
NgoMIV 1697 GCCGGC G^CCGGC
4051 GCCGGC
PaeR7I 1623 CTCGAG C^TCGAG
4365 CTCGAG
SapI 87 GAAGAGC GCTCTTC(1/4)
5662 GAAGAGC
SbfI 1292 CCTGCAGG CCTGCA^GG
4432 CCTGCAGG
SexAI 3796 ACCTGGT A^CC[AT]GGT
4760 ACCAGGT
SfoI 1000 GGCGCC GGC^GCC
2957 GGCGCC
Sse8387I 1292 CCTGCAGG CCTGCA^GG
4432 CCTGCAGG
StuI 2793 AGGCCT AGG^CCT
3530 AGGCCT
TaqI 1624 TCGA T^CGA
4366 TCGA
TthHB8I 1624 TCGA T^CGA
4366 TCGA
XhoI 1623 CTCGAG C^TCGAG
4365 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII
XmaCI XmaI