##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AscI Asp718I AspI BciVI
BfrI BsaBI BsaWI BseAI BsiWI
BspDI BspEI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EcoO65I EcoRV FseI HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NotI NspV PacI PaeR7I
PflFI PinAI PmeI Psp1406I PvuI
RsrII SalI SfuI SgrAI SnaBI
SpeI SphI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 3802 GACGTC GACGT^C
AcsI 2440 GAATTC [GA]^AATT[CT]
AhdI 6077 GACCGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4645 AGCGCT AGC^GCT
ApoI 2440 GAATTC [GA]^AATT[CT]
AspEI 6077 GACCGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3764 TGCGCA TGC^GCA
AvrII 5502 CCTAGG C^CTAGG
BamHI 405 GGATCC G^GATCC
BbsI 6630 GTCTTC GAAGAC(2/6)
BclI 4985 TGATCA T^GATCA
BlnI 5502 CCTAGG C^CTAGG
BpuAI 6630 GTCTTC GAAGAC(2/6)
BspHI 2474 TCATGA T^CATGA
BspLU11I 5921 ACATGT A^CATGT
EagI 5363 CGGCCG C^GGCCG
Eam1105I 6077 GACCGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5363 CGGCCG C^GGCCG
Eco47III 4645 AGCGCT AGC^GCT
Eco52I 5363 CGGCCG C^GGCCG
EcoNI 2371 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2440 GAATTC G^AATTC
FbaI 4985 TGATCA T^GATCA
FspI 3764 TGCGCA TGC^GCA
HincII 4576 GTCAAC GT[CT]^[GA]AC
HindII 4576 GTCAAC GT[CT]^[GA]AC
NaeI 5388 GCCGGC GCC^GGC
NgoMIV 5388 GCCGGC G^CCGGC
NheI 5903 GCTAGC G^CTAGC
RcaI 2474 TCATGA T^CATGA
SbfI 5217 CCTGCAGG CCTGCA^GG
ScaI 2825 AGTACT AGT^ACT
SexAI 5174 ACCTGGT A^CC[AT]GGT
SfiI 3501 GGCCTCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 934 CTTGAG C^T[CT][GA]AG
Sse8387I 5217 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 4618 GTGCAC G^TGCAC
4786 GTGCAC
ApaLI 4618 GTGCAC G^TGCAC
4786 GTGCAC
AseI 4771 ATTAAT AT^TAAT
6676 ATTAAT
AsnI 4771 ATTAAT AT^TAAT
6676 ATTAAT
Asp700I 1719 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2491 GAATCCATTC
BbrPI 4474 CACGTG CAC^GTG
4927 CACGTG
BbvCI 1056 CCTCAGC CCTCAGC(-5/-2)
2933 CCTCAGC
BsiEI 5363 CGGCCG CG[GA][CT]^CG
6130 CGACCG
BsmBI 6134 CGTCTC CGTCTC(1/5)
5638 GAGACG
BsmI 3822 GAATGC GAATGC(1/-1)
1634 GCATTC
BspMI 19 ACCTGC ACCTGC(4/8)
3446 ACCTGC
BssHII 3810 GCGCGC G^CGCGC
5305 GCGCGC
DraI 6351 TTTAAA TTT^AAA
6406 TTTAAA
EcoT22I 391 ATGCAT ATGCA^T
4715 ATGCAT
HindIII 804 AAGCTT A^AGCTT
2241 AAGCTT
KspI 5470 CCGCGG CCGC^GG
6151 CCGCGG
NruI 1944 TCGCGA TCG^CGA
5422 TCGCGA
NsiI 391 ATGCAT ATGCA^T
4715 ATGCAT
PflMI 1359 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4802 CCACCGGCTGG
PmaCI 4474 CACGTG CAC^GTG
4927 CACGTG
PmlI 4474 CACGTG CAC^GTG
4927 CACGTG
PshAI 313 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5500 GACCTAGGTC
PshBI 4771 ATTAAT AT^TAAT
6676 ATTAAT
SacII 5470 CCGCGG CCGC^GG
6151 CCGCGG
Van91I 1359 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4802 CCACCGGCTGG
XmnI 1719 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2491 GAATCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvI BcgI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI