##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbrPI BclI
BfrI BlnI BsaBI BsiEI BsiWI
BsmBI BspDI Bst1107I BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoRV FbaI FseI FspI KpnI
KspI MamI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SalI
ScaI SfiI SgrAI SnaBI SpeI
SphI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 4415 AACGTT AA^CGTT
Aor51HI 1964 AGCGCT AGC^GCT
BciVI 129 GGATAC GTATCC(6/5)
BglII 3953 AGATCT A^GATCT
BlpI 3319 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3319 GCTCAGC GC^T[ACGT]AGC
BsaAI 5927 TACGTG [CT]AC^GT[GA]
Bsp1407I 736 TGTACA T^GTACA
BspLU11I 4729 ACATGT A^CATGT
BsrBI 3057 CCGCTC CCGCTC(-3/-3)
BsrDI 1912 GCAATG GCAATG(2/0)
BsrFI 1522 ACCGGC [GA]^CCGG[CT]
BsrGI 736 TGTACA T^GTACA
BssHII 12 GCGCGC G^CGCGC
BstBI 428 TTCGAA TT^CGAA
CelII 3319 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1522 ACCGGC [GA]^CCGG[CT]
DraI 6002 TTTAAA TTT^AAA
DrdI 3552 GACAGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1964 AGCGCT AGC^GCT
EcoRI 1096 GAATTC G^AATTC
EcoT22I 911 ATGCAT ATGCA^T
HpaI 5864 GTTAAC GTT^AAC
NsiI 911 ATGCAT ATGCA^T
NspV 428 TTCGAA TT^CGAA
PaeR7I 2533 CTCGAG C^TCGAG
Psp1406I 4415 AACGTT AA^CGTT
SapI 3875 GAAGAGC GCTCTTC(1/4)
SbfI 3515 CCTGCAGG CCTGCA^GG
SfuI 428 TTCGAA TT^CGAA
SmaI 3016 CCCGGG CCC^GGG
Sse8387I 3515 CCTGCAGG CCTGCA^GG
SspBI 736 TGTACA T^GTACA
XhoI 2533 CTCGAG C^TCGAG
XmaCI 3016 CCCGGG C^CCGGG
XmaI 3016 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 949 TCCGGA T^CCGGA
2466 TCCGGA
Alw44I 1071 GTGCAC G^TGCAC
4918 GTGCAC
ApaLI 1071 GTGCAC G^TGCAC
4918 GTGCAC
AspI 1884 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4737 GACCCAGTC
BamHI 3521 GGATCC G^GATCC
3851 GGATCC
BbsI 3839 GAAGAC GAAGAC(2/6)
425 GTCTTC
BbvCI 3901 CCTCAGC CCTCAGC(-5/-2)
5312 CCTCAGC
BpuAI 3839 GAAGAC GAAGAC(2/6)
425 GTCTTC
BseAI 949 TCCGGA T^CCGGA
2466 TCCGGA
BspEI 949 TCCGGA T^CCGGA
2466 TCCGGA
BstEII 609 GGTCACC G^GT[ACGT]ACC
2614 GGTCACC
BstPI 609 GGTCACC G^GT[ACGT]ACC
2614 GGTCACC
EcoO65I 609 GGTCACC G^GT[ACGT]ACC
2614 GGTCACC
HindIII 101 AAGCTT A^AGCTT
861 AAGCTT
MroI 949 TCCGGA T^CCGGA
2466 TCCGGA
NdeI 3786 CATATG CA^TATG
3986 CATATG
NspI 877 GCATGT [GA]CATG^[CT]
4729 ACATGT
PflFI 1884 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4737 GACCCAGTC
PshAI 3405 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4232 GACCTCTGTC
SacI 1503 GAGCTC GAGCT^C
4886 GAGCTC
StuI 2887 AGGCCT AGG^CCT
4969 AGGCCT
Tth111I 1884 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4737 GACCCAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfoI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI