##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AflII Alw44I Aor51HI ApaLI AscI
AseI AsnI AspI AvrII BciVI
BfrI BlnI BspDI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI Eco47III
EcoRV FseI HindIII NaeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PmeI PshAI PshBI RsrII
SalI SfiI SfuI SgrAI SmaI
SnaBI SpeI SwaI Tth111I XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1361 GACGTC GACGT^C
Acc65I 3892 GGTACC G^GTACC
AccI 1364 GTCTAC GT^[AC][GT]AC
AclI 4492 AACGTT AA^CGTT
AgeI 3242 ACCGGT A^CCGGT
AocI 4311 CCTTAGG CC^T[ACGT]AGG
Asp718I 3892 GGTACC G^GTACC
AviII 981 TGCGCA TGC^GCA
BbrPI 341 CACGTG CAC^GTG
BcgI 2506 CGATGTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 6282 AGATCT A^GATCT
BlpI 2253 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2253 GCTCAGC GC^T[ACGT]AGC
BsaBI 6092 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 21 CGTACG C^GTACG
BsmI 4421 GAATGC GAATGC(1/-1)
BspLU11I 4973 ACATGT A^CATGT
BsrBI 1982 GAGCGG CCGCTC(-3/-3)
BsrDI 5082 CATTGC GCAATG(2/0)
BssSI 4513 CTCGTG CACGAG(-5/-1)
BstEII 406 GGTCACC G^GT[ACGT]ACC
BstPI 406 GGTCACC G^GT[ACGT]ACC
Bsu36I 4311 CCTTAGG CC^T[ACGT]AGG
CelII 2253 GCTCAGC GC^T[ACGT]AGC
EagI 1959 CGGCCG C^GGCCG
EclXI 1959 CGGCCG C^GGCCG
Eco52I 1959 CGGCCG C^GGCCG
Eco81I 4311 CCTTAGG CC^T[ACGT]AGG
EcoO65I 406 GGTCACC G^GT[ACGT]ACC
FspI 981 TGCGCA TGC^GCA
HpaI 4981 GTTAAC GTT^AAC
KpnI 3892 GGTACC GGTAC^C
KspI 4017 CCGCGG CCGC^GG
MamI 6092 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2670 CAATTG C^AATTG
MluI 18 ACGCGT A^CGCGT
MunI 2670 CAATTG C^AATTG
NdeI 1942 CATATG CA^TATG
PinAI 3242 ACCGGT A^CCGGT
PmaCI 341 CACGTG CAC^GTG
PmlI 341 CACGTG CAC^GTG
Psp1406I 4492 AACGTT AA^CGTT
PvuI 2478 CGATCG CGAT^CG
SacII 4017 CCGCGG CCGC^GG
SbfI 3934 CCTGCAGG CCTGCA^GG
ScaI 1697 AGTACT AGT^ACT
Sse8387I 3934 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccIII 1488 TCCGGA T^CCGGA
1533 TCCGGA
ApaI 2554 GGGCCC GGGCC^C
3695 GGGCCC
BseAI 1488 TCCGGA T^CCGGA
1533 TCCGGA
BsiEI 1959 CGGCCG CG[GA][CT]^CG
2478 CGATCG
BspEI 1488 TCCGGA T^CCGGA
1533 TCCGGA
BspHI 2050 TCATGA T^CATGA
4297 TCATGA
DrdI 263 GACCTGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2387 GACACCAAAGTC
MroI 1488 TCCGGA T^CCGGA
1533 TCCGGA
NcoI 3295 CCATGG C^CATGG
5116 CCATGG
PaeR7I 1103 CTCGAG C^TCGAG
3741 CTCGAG
RcaI 2050 TCATGA T^CATGA
4297 TCATGA
SapI 4608 GCTCTTC GCTCTTC(1/4)
6377 GCTCTTC
SphI 1395 GCATGC GCATG^C
5086 GCATGC
SspI 1682 AATATT AAT^ATT
6470 AATATT
StuI 447 AGGCCT AGG^CCT
667 AGGCCT
XhoI 1103 CTCGAG C^TCGAG
3741 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
ApoI Asp700I AspEI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbsI BbvCI BbvI BclI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI Eam1105I
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII XmnI