##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI BalI BbeI BbrPI
BcnI BglI BsaAI BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BsrBI BsrFI BssHII
BstBI BstEII BstPI Bsu36I Cfr10I
ClaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I FauI
FseI HgaI Hin1I KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PstI
PvuI RcaI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 3027 GTATAC GT^[AC][GT]AC
AccII 2568 CGCG CG^CG
AflII 4768 CTTAAG C^TTAAG
AviII 1679 TGCGCA TGC^GCA
BbvCI 45 GCTGAGG CCTCAGC(-5/-2)
BcgI 1002 CGAAGCAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4800 GTATCC GTATCC(6/5)
BfrI 4768 CTTAAG C^TTAAG
BlpI 702 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 702 GCTCAGC GC^T[ACGT]AGC
BsgI 3534 CTGCAC GTGCAG(16/14)
Bsp1407I 2941 TGTACA T^GTACA
BspMI 415 GCAGGT ACCTGC(4/8)
BsrGI 2941 TGTACA T^GTACA
Bst1107I 3027 GTATAC GTA^TAC
BstUI 2568 CGCG CG^CG
BstZ17I 3027 GTATAC GTA^TAC
CelII 702 GCTCAGC GC^T[ACGT]AGC
CpoI 5791 CGGTCCG CG^G[AT]CCG
DrdI 4105 GACTGCATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1679 TGCGCA TGC^GCA
HaeII 1443 AGCGCC [GA]GCGC^[CT]
HpaI 1225 GTTAAC GTT^AAC
MvnI 2568 CGCG CG^CG
PshAI 657 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 5505 CAGCTG CAG^CTG
RsrII 5791 CGGTCCG CG^G[AT]CCG
SacI 466 GAGCTC GAGCT^C
SapI 1390 GAAGAGC GCTCTTC(1/4)
SpeI 2053 ACTAGT A^CTAGT
SspBI 2941 TGTACA T^GTACA
SspI 2245 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AseI 3357 ATTAAT AT^TAAT
3391 ATTAAT
AsnI 3357 ATTAAT AT^TAAT
3391 ATTAAT
Asp700I 1744 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5189 GAATAAATTC
AspHI 466 GAGCTC G[AT]GC[AT]^C
3480 GTGCTC
AvrII 4292 CCTAGG C^CTAGG
5802 CCTAGG
BanII 466 GAGCTC G[GA]GC[CT]^C
3443 GAGCCC
BbsI 1539 GAAGAC GAAGAC(2/6)
5174 GAAGAC
BlnI 4292 CCTAGG C^CTAGG
5802 CCTAGG
BpuAI 1539 GAAGAC GAAGAC(2/6)
5174 GAAGAC
BsaBI 676 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3972 GATGAAAATC
BsaI 2879 GAGACC GGTCTC(1/5)
3667 GAGACC
BsaWI 1185 TCCGGT [AT]^CCGG[AT]
5789 TCCGGT
BsiHKAI 466 GAGCTC G[AT]GC[AT]^C
3480 GTGCTC
BsrDI 1275 GCAATG GCAATG(2/0)
1927 GCAATG
BssSI 2537 CTCGTG CACGAG(-5/-1)
3411 CTCGTG
DraII 1836 GGGTCCC [GA]G^G[ACGT]CC[CT]
4281 AGGACCC
DraIII 4233 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4989 CACTTAGTG
EcoO109I 1836 GGGTCCC [GA]G^G[ACGT]CC[CT]
4281 AGGACCC
EcoT22I 2074 ATGCAT ATGCA^T
2778 ATGCAT
HincII 1225 GTTAAC GT[CT]^[GA]AC
5333 GTCAAC
HindII 1225 GTTAAC GT[CT]^[GA]AC
5333 GTCAAC
MamI 676 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3972 GATGAAAATC
MspA1I 1717 CAGCGG C[AC]G^C[GT]G
5505 CAGCTG
NdeI 5688 CATATG CA^TATG
6107 CATATG
NsiI 2074 ATGCAT ATGCA^T
2778 ATGCAT
PpuMI 1836 GGGTCCC [GA]G^G[AT]CC[CT]
4281 AGGACCC
PshBI 3357 ATTAAT AT^TAAT
3391 ATTAAT
ScaI 733 AGTACT AGT^ACT
2353 AGTACT
SexAI 1434 ACCTGGT A^CC[AT]GGT
5723 ACCAGGT
SfcI 881 CTACAG C^T[GA][CT]AG
5601 CTGTAG
SphI 2076 GCATGC GCATG^C
5342 GCATGC
StuI 2422 AGGCCT AGG^CCT
4634 AGGCCT
XbaI 3309 TCTAGA T^CTAGA
4624 TCTAGA
XcmI 3788 CCAATCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4084 CCAGCCCCTTTGTGG
XmnI 1744 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5189 GAATAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AvaII BamHI
BanI BbvI BclI BfaI BglII
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspLU11I BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EarI EcoNI EcoRI EcoRII EcoRV
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII