##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccIII AclI AflIII AgeI Aor51HI
AscI AspI AvrII BbeI BbrPI
BlnI BseAI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BssHII BssSI
BstBI ClaI CpoI DraIII DrdI
Eco47III EcoRI FseI HindIII HpaI
KasI MluI MroI NarI NotI
NruI NspV PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SalI SbfI SexAI SfiI
SfoI SfuI SmaI Sse8387I SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 951 GGTACC G^GTACC
AhdI 5238 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2266 GTGCAC G^TGCAC
AocI 1692 CCTGAGG CC^T[ACGT]AGG
ApaLI 2266 GTGCAC G^TGCAC
AseI 1487 ATTAAT AT^TAAT
AsnI 1487 ATTAAT AT^TAAT
Asp700I 5464 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 951 GGTACC G^GTACC
AspEI 5238 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1082 TGCGCA TGC^GCA
BalI 6098 TGGCCA TGG^CCA
BamHI 1776 GGATCC G^GATCC
BcgI 56 CGACTCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1309 TACGTA [CT]AC^GT[GA]
BsiEI 1 CGGCCG CG[GA][CT]^CG
Bsp1407I 3893 TGTACA T^GTACA
BsrBI 6378 CCGCTC CCGCTC(-3/-3)
BsrGI 3893 TGTACA T^GTACA
Bst1107I 5357 GTATAC GTA^TAC
BstAPI 4650 GCAGACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 5502 GGTAACC G^GT[ACGT]ACC
BstPI 5502 GGTAACC G^GT[ACGT]ACC
BstZ17I 5357 GTATAC GTA^TAC
Bsu36I 1692 CCTGAGG CC^T[ACGT]AGG
EagI 1 CGGCCG C^GGCCG
Eam1105I 5238 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1 CGGCCG C^GGCCG
Eco52I 1 CGGCCG C^GGCCG
Eco81I 1692 CCTGAGG CC^T[ACGT]AGG
EcoO65I 5502 GGTAACC G^GT[ACGT]ACC
EcoT22I 1172 ATGCAT ATGCA^T
FspI 1082 TGCGCA TGC^GCA
KpnI 951 GGTACC GGTAC^C
KspI 121 CCGCGG CCGC^GG
MluNI 6098 TGGCCA TGG^CCA
MscI 6098 TGGCCA TGG^CCA
NaeI 335 GCCGGC GCC^GGC
NdeI 1825 CATATG CA^TATG
NgoMIV 335 GCCGGC G^CCGGC
NheI 1623 GCTAGC G^CTAGC
NsiI 1172 ATGCAT ATGCA^T
PacI 1488 TTAATTAA TTAAT^TAA
PaeR7I 1798 CTCGAG C^TCGAG
PshBI 1487 ATTAAT AT^TAAT
SacII 121 CCGCGG CCGC^GG
SapI 411 GAAGAGC GCTCTTC(1/4)
SgrAI 334 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 1309 TACGTA TAC^GTA
SspBI 3893 TGTACA T^GTACA
SspI 633 AATATT AAT^ATT
StuI 2971 AGGCCT AGG^CCT
XcmI 3220 CCAGCACAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1798 CTCGAG C^TCGAG
XmnI 5464 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 710 GACGTC GACGT^C
5768 GACGTC
AcyI 710 GACGTC G[GA]^CG[CT]C
5768 GACGTC
AflII 3074 CTTAAG C^TTAAG
5607 CTTAAG
BbvCI 2013 CCTCAGC CCTCAGC(-5/-2)
6249 GCTGAGG
BciVI 3398 GTATCC GTATCC(6/5)
3853 GTATCC
BfrI 3074 CTTAAG C^TTAAG
5607 CTTAAG
BglII 1087 AGATCT A^GATCT
6225 AGATCT
BsaHI 710 GACGTC G[GA]^CG[CT]C
5768 GACGTC
BsmI 6245 GAATGC GAATGC(1/-1)
2779 GCATTC
BsrDI 3083 CATTGC GCAATG(2/0)
2302 GCAATG
DraI 591 TTTAAA TTT^AAA
744 TTTAAA
EcoNI 3714 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4395 CCTTTTCCAGG
EcoRV 405 GATATC GAT^ATC
5250 GATATC
HaeII 363 AGCGCC [GA]GCGC^[CT]
6054 GGCGCT
HgaI 46 GCGTC GACGC(5/10)
2754 GCGTC
Hin1I 710 GACGTC G[GA]^CG[CT]C
5768 GACGTC
MfeI 4452 CAATTG C^AATTG
5819 CAATTG
MunI 4452 CAATTG C^AATTG
5819 CAATTG
PshAI 900 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1764 GACAGTGGTC
SphI 1030 GCATGC GCATG^C
4073 GCATGC
XbaI 4019 TCTAGA T^CTAGA
4433 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SpeI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII