##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI Asp718I
AspEI BfrI BlpI Bpu1102I BsaWI
BseAI BsiWI BspDI BspEI BspHI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI Eam1105I Eco47III EcoRI
FseI HindIII HpaI KpnI MluI
MroI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmeI Psp1406I
PvuI RcaI RsrII SalI SfiI
SfuI SgrAI SnaBI SpeI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AseI 4905 ATTAAT AT^TAAT
AsnI 4905 ATTAAT AT^TAAT
Asp700I 2588 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 6151 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1624 GGATCC G^GATCC
BclI 1947 TGATCA T^GATCA
BsaI 3568 GAGACC GGTCTC(1/5)
BsiEI 6935 CGGCCG CG[GA][CT]^CG
BspLU11I 5697 ACATGT A^CATGT
BstAPI 3207 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 310 GGTGACC G^GT[ACGT]ACC
BstPI 310 GGTGACC G^GT[ACGT]ACC
DraI 2156 TTTAAA TTT^AAA
EagI 6935 CGGCCG C^GGCCG
EclXI 6935 CGGCCG C^GGCCG
Eco52I 6935 CGGCCG C^GGCCG
EcoO65I 310 GGTGACC G^GT[ACGT]ACC
EcoRV 3967 GATATC GAT^ATC
EcoT22I 1611 ATGCAT ATGCA^T
FbaI 1947 TGATCA T^GATCA
MfeI 7150 CAATTG C^AATTG
MunI 7150 CAATTG C^AATTG
NsiI 1611 ATGCAT ATGCA^T
PflFI 6151 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1793 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4905 ATTAAT AT^TAAT
SapI 3296 GAAGAGC GCTCTTC(1/4)
SbfI 2839 CCTGCAGG CCTGCA^GG
ScaI 504 AGTACT AGT^ACT
SmaI 3123 CCCGGG CCC^GGG
SphI 5683 GCATGC GCATG^C
Sse8387I 2839 CCTGCAGG CCTGCA^GG
SspI 2111 AATATT AAT^ATT
StuI 1413 AGGCCT AGG^CCT
Tth111I 6151 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3123 CCCGGG C^CCGGG
XmaI 3123 CCCGGG C^CCGGG
XmnI 2588 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1385 GTCTAC GT^[AC][GT]AC
3059 GTCTAC
AcsI 797 AAATTC [GA]^AATT[CT]
2324 AAATTT
AflIII 678 ACGTGT A^C[GA][CT]GT
5697 ACATGT
ApoI 797 AAATTC [GA]^AATT[CT]
2324 AAATTT
AviII 264 TGCGCA TGC^GCA
1987 TGCGCA
BbrPI 2030 CACGTG CAC^GTG
5688 CACGTG
BglI 2941 GCCCCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6932 GCCCGGCCGGC
BglII 245 AGATCT A^GATCT
3025 AGATCT
BsaAI 2030 CACGTG [CT]AC^GT[GA]
5688 CACGTG
BsaBI 246 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2401 GATGACCATC
BsmBI 3710 CGTCTC CGTCTC(1/5)
3918 CGTCTC
Bsp1407I 1281 TGTACA T^GTACA
5700 TGTACA
BsrGI 1281 TGTACA T^GTACA
5700 TGTACA
DrdI 665 GACATGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
736 GACAGCAGTGTC
FspI 264 TGCGCA TGC^GCA
1987 TGCGCA
HincII 211 GTCAAC GT[CT]^[GA]AC
674 GTCAAC
HindII 211 GTCAAC GT[CT]^[GA]AC
674 GTCAAC
MamI 246 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2401 GATGACCATC
NaeI 5546 GCCGGC GCC^GGC
6937 GCCGGC
NgoMIV 5546 GCCGGC G^CCGGC
6937 GCCGGC
NheI 1175 GCTAGC G^CTAGC
1810 GCTAGC
PmaCI 2030 CACGTG CAC^GTG
5688 CACGTG
PmlI 2030 CACGTG CAC^GTG
5688 CACGTG
SexAI 4080 ACCTGGT A^CC[AT]GGT
5927 ACCTGGT
SspBI 1281 TGTACA T^GTACA
5700 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII AvrII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BciVI BcnI
BfaI BlnI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII