##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

AccIII      AclI        AflII       AgeI        AhdI        
Aor51HI     AscI        AspEI       AviII       BfrI        
BsaWI       BseAI       BsiWI       BspDI       BspEI       
BsrBI       BssHII      BstAPI      BstEII      BstPI       
ClaI        CpoI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoT22I     
FauI        FseI        FspI        KspI        MluI        
MroI        MspA1I      NotI        NsiI        PacI        
PaeR7I      PinAI       PshAI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SapI        SfiI        
SgrAI       SnaBI       SphI        StuI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII        4916  GACGTC               GACGT^C
AocI         4382  CCTCAGG              CC^T[ACGT]AGG
ApaI          262  GGGCCC               GGGCC^C
BamHI        1452  GGATCC               G^GATCC
BbeI          203  GGCGCC               GGCGC^C
BclI         5138  TGATCA               T^GATCA
BglII        2006  AGATCT               A^GATCT
BsaBI        4361  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         904  GAGGAG               GAGGAG(10/8)
BsiEI        4175  CGACCG               CG[GA][CT]^CG
BsmBI         329  GAGACG               CGTCTC(1/5)
Bsp1407I     2722  TGTACA               T^GTACA
BsrFI         393  GCCGGC               [GA]^CCGG[CT]
BsrGI        2722  TGTACA               T^GTACA
BssSI        1893  CACGAG               CACGAG(-5/-1)
BstBI        1399  TTCGAA               TT^CGAA
Bsu36I       4382  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        393  GCCGGC               [GA]^CCGG[CT]
DrdI         5078  GACTCAAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         5282  CTCTTC               CTCTTC(1/4)
Eco81I       4382  CCTCAGG              CC^T[ACGT]AGG
FbaI         5138  TGATCA               T^GATCA
HaeII         203  GGCGCC               [GA]GCGC^[CT]
HgaI         5202  GCGTC                GACGC(5/10)
HpaI         4812  GTTAAC               GTT^AAC
KasI          203  GGCGCC               G^GCGCC
Ksp632I      5282  CTCTTC               CTCTTC(1/4)
MamI         4361  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI          393  GCCGGC               GCC^GGC
NarI          203  GGCGCC               GG^CGCC
NcoI         4612  CCATGG               C^CATGG
NdeI         1271  CATATG               CA^TATG
NgoMIV        393  GCCGGC               G^CCGGC
NheI         1222  GCTAGC               G^CTAGC
NruI         3517  TCGCGA               TCG^CGA
NspV         1399  TTCGAA               TT^CGAA
SacI         4161  GAGCTC               GAGCT^C
SbfI         2245  CCTGCAGG             CCTGCA^GG
ScaI         5806  AGTACT               AGT^ACT
SexAI        4489  ACCAGGT              A^CC[AT]GGT
SfoI          203  GGCGCC               GGC^GCC
SfuI         1399  TTCGAA               TT^CGAA
SmaI          235  CCCGGG               CCC^GGG
Sse8387I     2245  CCTGCAGG             CCTGCA^GG
SspBI        2722  TGTACA               T^GTACA
SwaI         2075  ATTTAAAT             ATTT^AAAT
XcmI         5617  CCAGTCTGTGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         235  CCCGGG               C^CCGGG
XmaI          235  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       4710  GGTACC               G^GTACC
             4833  GGTACC              
AcyI          203  GGCGCC               G[GA]^CG[CT]C
             4916  GACGTC              
Alw44I       1356  GTGCAC               G^TGCAC
             3038  GTGCAC              
AlwNI        1012  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             4624  CAGAACCTG           
ApaLI        1356  GTGCAC               G^TGCAC
             3038  GTGCAC              
Asp718I      4710  GGTACC               G^GTACC
             4833  GGTACC              
AspI           54  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              165  GACGGGGTC           
AvrII         618  CCTAGG               C^CTAGG
              675  CCTAGG              
BbrPI         289  CACGTG               CAC^GTG
             6184  CACGTG              
BbvCI        1604  CCTCAGC              CCTCAGC(-5/-2)
             3689  CCTCAGC             
BciVI        2265  GGATAC               GTATCC(6/5)
             4366  GTATCC              
BglI          206  GCCATCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3261  GCCAGCATGGC         
BlnI          618  CCTAGG               C^CTAGG
              675  CCTAGG              
BlpI         3252  GCTCAGC              GC^T[ACGT]AGC
             4617  GCTGAGC             
Bpu1102I     3252  GCTCAGC              GC^T[ACGT]AGC
             4617  GCTGAGC             
BsaAI         289  CACGTG               [CT]AC^GT[GA]
             6184  CACGTG              
BsaHI         203  GGCGCC               G[GA]^CG[CT]C
             4916  GACGTC              
BsaI         1961  GAGACC               GGTCTC(1/5)
              359  GGTCTC              
BsgI           26  CTGCAC               GTGCAG(16/14)
              196  GTGCAG              
BspMI        1529  GCAGGT               ACCTGC(4/8)
             2248  GCAGGT              
BsrDI        5207  CATTGC               GCAATG(2/0)
             3439  GCAATG              
Bst1107I      603  GTATAC               GTA^TAC
             2709  GTATAC              
BstYI        1452  GGATCC               [GA]^GATC[CT]
             2006  AGATCT              
BstZ17I       603  GTATAC               GTA^TAC
             2709  GTATAC              
CelII        3252  GCTCAGC              GC^T[ACGT]AGC
             4617  GCTGAGC             
DraIII       4488  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             6184  CACGTGGTG           
DsaI         3028  CCACGG               C^C[GA][CT]GG
             4612  CCATGG              
EcoRV        4541  GATATC               GAT^ATC
             4578  GATATC              
Hin1I         203  GGCGCC               G[GA]^CG[CT]C
             4916  GACGTC              
KpnI         4710  GGTACC               GGTAC^C
             4833  GGTACC              
MfeI          723  CAATTG               C^AATTG
             3300  CAATTG              
MflI         1452  GGATCC               [GA]^GATC[CT]
             2006  AGATCT              
MunI          723  CAATTG               C^AATTG
             3300  CAATTG              
PflFI          54  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              165  GACGGGGTC           
PmaCI         289  CACGTG               CAC^GTG
             6184  CACGTG              
PmeI         1262  GTTTAAAC             GTTT^AAAC
             4567  GTTTAAAC            
PmlI          289  CACGTG               CAC^GTG
             6184  CACGTG              
PpuMI        4818  GGGACCC              [GA]G^G[AT]CC[CT]
             4984  GGGTCCC             
SalI         4173  GTCGAC               G^TCGAC
             5385  GTCGAC              
Tth111I        54  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              165  GACGGGGTC           
XhoII        1452  GGATCC               [GA]^GATC[CT]
             2006  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AflIII      AluI        AlwI        ApoI        AseI        
AsnI        Asp700I     AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcgI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspHI       BspLU11I    BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EaeI        EcoO109I    EcoRI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HindIII     HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PshBI       
PstI        RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SpeI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XmnI