##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AocI
ApaLI AseI AsnI Asp700I Asp718I
AspI BbrPI BbvCI BciVI BclI
BfrI BglII BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AhdI 646 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1960 AGCGCT AGC^GCT
AscI 769 GGCGCGCC GG^CGCGCC
AspEI 646 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 329 TGCGCA TGC^GCA
AvrII 2688 CCTAGG C^CTAGG
BalI 122 TGGCCA TGG^CCA
BcgI 1140 GCAATCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2688 CCTAGG C^CTAGG
BsgI 2079 GTGCAG GTGCAG(16/14)
BsrBI 1954 CCGCTC CCGCTC(-3/-3)
BsrDI 2346 GCAATG GCAATG(2/0)
Eam1105I 646 GACGAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1960 AGCGCT AGC^GCT
FspI 329 TGCGCA TGC^GCA
MaeII 1188 ACGT A^CGT
MluNI 122 TGGCCA TGG^CCA
MscI 122 TGGCCA TGG^CCA
MslI 2502 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1441 CCGCTG C[AC]G^C[GT]G
NspI 1697 ACATGC [GA]CATG^[CT]
SacI 2276 GAGCTC GAGCT^C
SapI 1395 GCTCTTC GCTCTTC(1/4)
SfaNI 709 GCATC GCATC(5/9)
TfiI 1573 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 1781 GAATTT [GA]^AATT[CT]
2739 AAATTC
AfaI 552 GTAC GT^AC
2609 GTAC
AlwNI 1428 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2256 CAGCTCCTG
ApoI 1781 GAATTT [GA]^AATT[CT]
2739 AAATTC
BamHI 973 GGATCC G^GATCC
2721 GGATCC
BbsI 1691 GAAGAC GAAGAC(2/6)
464 GTCTTC
BfaI 1035 CTAG C^TAG
2689 CTAG
BlpI 1956 GCTCAGC GC^T[ACGT]AGC
2273 GCTGAGC
Bpu1102I 1956 GCTCAGC GC^T[ACGT]AGC
2273 GCTGAGC
BpuAI 1691 GAAGAC GAAGAC(2/6)
464 GTCTTC
BsaI 230 GAGACC GGTCTC(1/5)
2524 GAGACC
CelII 1956 GCTCAGC GC^T[ACGT]AGC
2273 GCTGAGC
DsaI 320 CCGTGG C^C[GA][CT]GG
2305 CCACGG
MaeI 1035 CTAG C^TAG
2689 CTAG
MseI 1856 TTAA T^TAA
2023 TTAA
NaeI 766 GCCGGC GCC^GGC
2087 GCCGGC
NgoMIV 766 GCCGGC G^CCGGC
2087 GCCGGC
PflMI 495 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2375 CCATTCCCTGG
RsaI 552 GTAC GT^AC
2609 GTAC
SfiI 123 GGCCATTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2028 GGCCAGAGGGGCC
SmlI 2213 CTTGAG C^T[CT][GA]AG
2613 CTTGAG
TaqI 194 TCGA T^CGA
2280 TCGA
Tru9I 1856 TTAA T^TAA
2023 TTAA
TthHB8I 194 TCGA T^CGA
2280 TCGA
Van91I 495 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2375 CCATTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
ApaI AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeIII MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI Sau3AI Sau96I ScrFI
SfcI SfoI SmaI StyI TseI
Tsp45I Tsp509I TspRI XcmI XhoII
XmaCI XmaI