##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI ApaI AscI
AseI AsnI AspEI AviII AvrII
BbrPI BbsI BbvCI BclI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaI BsaWI BseAI BsiWI BspDI
BspEI BspLU11I BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraIII Eam1105I EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HpaI MfeI MluI MroI MunI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SalI SapI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SspI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 2001 GGTACC G^GTACC
AcyI 34 GGCGCC G[GA]^CG[CT]C
Alw44I 1763 GTGCAC G^TGCAC
AocI 1658 CCTCAGG CC^T[ACGT]AGG
Aor51HI 285 AGCGCT AGC^GCT
ApaLI 1763 GTGCAC G^TGCAC
Asp718I 2001 GGTACC G^GTACC
AspI 1990 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 12 CTCGGG C^[CT]CG[GA]G
BalI 1818 TGGCCA TGG^CCA
BamHI 1960 GGATCC G^GATCC
BbeI 34 GGCGCC GGCGC^C
BcgI 1571 GCAACACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3042 GTATCC GTATCC(6/5)
BglII 2485 AGATCT A^GATCT
BpmI 862 CTGGAG CTGGAG(16/14)
BsaBI 2137 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 34 GGCGCC G[GA]^CG[CT]C
BsiEI 159 CGGCCG CG[GA][CT]^CG
BsmBI 1664 GAGACG CGTCTC(1/5)
BsmI 1431 GAATGC GAATGC(1/-1)
BsoBI 12 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2151 TGTACA T^GTACA
BspHI 2405 TCATGA T^CATGA
BspMI 579 ACCTGC ACCTGC(4/8)
BsrGI 2151 TGTACA T^GTACA
BsrI 1202 ACTGG ACTGG(1/-1)
BssHII 135 GCGCGC G^CGCGC
BstXI 1612 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1658 CCTCAGG CC^T[ACGT]AGG
DrdI 1127 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 159 CGGCCG C^GGCCG
EclXI 159 CGGCCG C^GGCCG
Eco47III 285 AGCGCT AGC^GCT
Eco52I 159 CGGCCG C^GGCCG
Eco81I 1658 CCTCAGG CC^T[ACGT]AGG
EcoNI 841 CCTCCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 728 GATATC GAT^ATC
Hin1I 34 GGCGCC G[GA]^CG[CT]C
HincII 1867 GTCAAC GT[CT]^[GA]AC
HindII 1867 GTCAAC GT[CT]^[GA]AC
HindIII 1966 AAGCTT A^AGCTT
KasI 34 GGCGCC G^GCGCC
KpnI 2001 GGTACC GGTAC^C
MamI 2137 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1818 TGGCCA TGG^CCA
MscI 1818 TGGCCA TGG^CCA
NaeI 166 GCCGGC GCC^GGC
NarI 34 GGCGCC GG^CGCC
NcoI 2296 CCATGG C^CATGG
NdeI 2250 CATATG CA^TATG
NgoMIV 166 GCCGGC G^CCGGC
NheI 2325 GCTAGC G^CTAGC
PflFI 1990 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2405 TCATGA T^CATGA
SacI 1300 GAGCTC GAGCT^C
SbfI 580 CCTGCAGG CCTGCA^GG
ScaI 914 AGTACT AGT^ACT
SfoI 34 GGCGCC GGC^GCC
Sse8387I 580 CCTGCAGG CCTGCA^GG
SspBI 2151 TGTACA T^GTACA
StuI 1823 AGGCCT AGG^CCT
Tth111I 1990 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1096 CCAGCCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 1197 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
2821 CAGAAACTG
Asp700I 533 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2287 GAAGTGTTTC
AspHI 1300 GAGCTC G[AT]GC[AT]^C
1763 GTGCAC
BanII 962 GGGCTC G[GA]GC[CT]^C
1300 GAGCTC
BglI 2 GCCGCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
161 GCCGGGCCGGC
BsiHKAI 1300 GAGCTC G[AT]GC[AT]^C
1763 GTGCAC
BsmAI 1664 GAGAC GTCTC(1/5)
501 GTCTC
BsrFI 166 GCCGGC [GA]^CCGG[CT]
1837 GCCGGT
BstYI 1960 GGATCC [GA]^GATC[CT]
2485 AGATCT
Cfr10I 166 GCCGGC [GA]^CCGG[CT]
1837 GCCGGT
DraII 15 GGGACCC [GA]G^G[ACGT]CC[CT]
1549 AGGACCC
EcoO109I 15 GGGACCC [GA]G^G[ACGT]CC[CT]
1549 AGGACCC
KspI 3 CCGCGG CCGC^GG
72 CCGCGG
MflI 1960 GGATCC [GA]^GATC[CT]
2485 AGATCT
PflMI 1100 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1385 CCAGGAAGTGG
PpuMI 15 GGGACCC [GA]G^G[AT]CC[CT]
1549 AGGACCC
SacII 3 CCGCGG CCGC^GG
72 CCGCGG
SmlI 1316 CTCAAG C^T[CT][GA]AG
2436 CTTGAG
SphI 1033 GCATGC GCATG^C
1567 GCATGC
Van91I 1100 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1385 CCAGGAAGTGG
XhoII 1960 GGATCC [GA]^GATC[CT]
2485 AGATCT
XmnI 533 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2287 GAAGTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BanI BbvI
BcnI BfaI BmyI BsaJI BseRI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrBI BsrDI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I