##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AclI Asp700I AviII BglII BsaBI
BsiWI BspDI BspHI Bst1107I BstBI
BstZ17I ClaI CpoI DraI EcoRI
EcoRV EcoT22I FseI FspI MamI
MfeI MunI NheI NotI NruI
NsiI NspV PacI PmeI PshAI
Psp1406I PvuI RcaI RsrII SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 55 GACGTC GACGT^C
AccIII 5903 TCCGGA T^CCGGA
AgeI 1028 ACCGGT A^CCGGT
Alw44I 3876 GTGCAC G^TGCAC
ApaLI 3876 GTGCAC G^TGCAC
AscI 718 GGCGCGCC GG^CGCGCC
AseI 1800 ATTAAT AT^TAAT
AsnI 1800 ATTAAT AT^TAAT
BbrPI 674 CACGTG CAC^GTG
BcgI 3820 GCACGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3565 GGATAC GTATCC(6/5)
BclI 4528 TGATCA T^GATCA
BseAI 5903 TCCGGA T^CCGGA
BspEI 5903 TCCGGA T^CCGGA
BstEII 4059 GGTCACC G^GT[ACGT]ACC
BstPI 4059 GGTCACC G^GT[ACGT]ACC
EcoO65I 4059 GGTCACC G^GT[ACGT]ACC
FbaI 4528 TGATCA T^GATCA
HindIII 4759 AAGCTT A^AGCTT
HpaI 2955 GTTAAC GTT^AAC
MroI 5903 TCCGGA T^CCGGA
NaeI 1449 GCCGGC GCC^GGC
NdeI 2268 CATATG CA^TATG
NgoMIV 1449 GCCGGC G^CCGGC
PaeR7I 79 CTCGAG C^TCGAG
PinAI 1028 ACCGGT A^CCGGT
PmaCI 674 CACGTG CAC^GTG
PmlI 674 CACGTG CAC^GTG
PshBI 1800 ATTAAT AT^TAAT
SacI 4703 GAGCTC GAGCT^C
SalI 1094 GTCGAC G^TCGAC
ScaI 6413 AGTACT AGT^ACT
SmaI 4509 CCCGGG CCC^GGG
SphI 3424 GCATGC GCATG^C
XhoI 79 CTCGAG C^TCGAG
XmaCI 4509 CCCGGG C^CCGGG
XmaI 4509 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 2588 GGTACC G^GTACC
6404 GGTACC
AccI 1094 GTCGAC GT^[AC][GT]AC
6180 GTCTAC
AocI 2427 CCTGAGG CC^T[ACGT]AGG
5470 CCTCAGG
Aor51HI 1362 AGCGCT AGC^GCT
3241 AGCGCT
Asp718I 2588 GGTACC G^GTACC
6404 GGTACC
AvrII 3342 CCTAGG C^CTAGG
5510 CCTAGG
BbsI 4986 GAAGAC GAAGAC(2/6)
5951 GAAGAC
BlnI 3342 CCTAGG C^CTAGG
5510 CCTAGG
BpuAI 4986 GAAGAC GAAGAC(2/6)
5951 GAAGAC
BsaWI 1028 ACCGGT [AT]^CCGG[AT]
5903 TCCGGA
BsmI 1008 GAATGC GAATGC(1/-1)
4778 GAATGC
Bsp1407I 1635 TGTACA T^GTACA
6167 TGTACA
BsrBI 407 GAGCGG CCGCTC(-3/-3)
704 GAGCGG
BsrGI 1635 TGTACA T^GTACA
6167 TGTACA
Bsu36I 2427 CCTGAGG CC^T[ACGT]AGG
5470 CCTCAGG
Eco47III 1362 AGCGCT AGC^GCT
3241 AGCGCT
Eco81I 2427 CCTGAGG CC^T[ACGT]AGG
5470 CCTCAGG
KpnI 2588 GGTACC GGTAC^C
6404 GGTACC
MluI 4171 ACGCGT A^CGCGT
6271 ACGCGT
PflMI 3656 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3943 CCATTACCTGG
SapI 4017 GAAGAGC GCTCTTC(1/4)
5212 GCTCTTC
SspBI 1635 TGTACA T^GTACA
6167 TGTACA
Van91I 3656 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3943 CCATTACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AhdI AluI AlwI
AlwNI ApaI ApoI AspEI AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BfrI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrDI BsrFI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII