################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI AspEI AspI AvrII BbeI BbsI BciVI BclI BglII BlnI BpuAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoRI EcoRV FbaI FseI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspI SwaI Tsp509I Tth111I XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 251 GGTACC G^GTACC AccI 688 GTCTAC GT^[AC][GT]AC AflII 2844 CTTAAG C^TTAAG AflIII 2812 ACATGT A^C[GA][CT]GT Aor51HI 992 AGCGCT AGC^GCT Asp718I 251 GGTACC G^GTACC AviII 275 TGCGCA TGC^GCA BamHI 2177 GGATCC G^GATCC BbrPI 966 CACGTG CAC^GTG BbvCI 534 CCTCAGC CCTCAGC(-5/-2) BfrI 2844 CTTAAG C^TTAAG BsaAI 966 CACGTG [CT]AC^GT[GA] Bsp1407I 230 TGTACA T^GTACA BspLU11I 2812 ACATGT A^CATGT BsrDI 782 GCAATG GCAATG(2/0) BsrGI 230 TGTACA T^GTACA Eco47III 992 AGCGCT AGC^GCT EcoT22I 2867 ATGCAT ATGCA^T FspI 275 TGCGCA TGC^GCA HgaI 41 GCGTC GACGC(5/10) KpnI 251 GGTACC GGTAC^C NcoI 1554 CCATGG C^CATGG NsiI 2867 ATGCAT ATGCA^T PmaCI 966 CACGTG CAC^GTG PmlI 966 CACGTG CAC^GTG SfiI 1942 GGCCAAAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 2556 ACTAGT A^CTAGT SspBI 230 TGTACA T^GTACA StuI 1499 AGGCCT AGG^CCT XbaI 2829 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AocI 574 CCTCAGG CC^T[ACGT]AGG 2374 CCTCAGG Asp700I 166 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2638 GAATGCCTTC AspHI 801 GAGCTC G[AT]GC[AT]^C 1105 GAGCTC BalI 666 TGGCCA TGG^CCA 1754 TGGCCA BlpI 798 GCTGAGC GC^T[ACGT]AGC 1571 GCTCAGC Bpu1102I 798 GCTGAGC GC^T[ACGT]AGC 1571 GCTCAGC BsaI 790 GAGACC GGTCTC(1/5) 1391 GGTCTC BsiHKAI 801 GAGCTC G[AT]GC[AT]^C 1105 GAGCTC BsmI 1744 GAATGC GAATGC(1/-1) 2638 GAATGC BspHI 855 TCATGA T^CATGA 2491 TCATGA BsrFI 539 GCCGGC [GA]^CCGG[CT] 1112 GCCGGC BstEII 471 GGTCACC G^GT[ACGT]ACC 2454 GGTGACC BstPI 471 GGTCACC G^GT[ACGT]ACC 2454 GGTGACC BstXI 1617 CCACTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2189 CCATCCGGCTGG Bsu36I 574 CCTCAGG CC^T[ACGT]AGG 2374 CCTCAGG CelII 798 GCTGAGC GC^T[ACGT]AGC 1571 GCTCAGC Cfr10I 539 GCCGGC [GA]^CCGG[CT] 1112 GCCGGC DrdI 1636 GACTCTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1841 GACACTGGAGTC DsaI 1554 CCATGG C^C[GA][CT]GG 1978 CCACGG Eco81I 574 CCTCAGG CC^T[ACGT]AGG 2374 CCTCAGG EcoO65I 471 GGTCACC G^GT[ACGT]ACC 2454 GGTGACC MaeII 967 ACGT A^CGT 2216 ACGT MluNI 666 TGGCCA TGG^CCA 1754 TGGCCA MscI 666 TGGCCA TGG^CCA 1754 TGGCCA MseI 2845 TTAA T^TAA 2946 TTAA NaeI 539 GCCGGC GCC^GGC 1112 GCCGGC NgoMIV 539 GCCGGC G^CCGGC 1112 GCCGGC PflMI 1020 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1092 CCAGCTCCTGG RcaI 855 TCATGA T^CATGA 2491 TCATGA SacI 801 GAGCTC GAGCT^C 1105 GAGCTC SapI 1102 GAAGAGC GCTCTTC(1/4) 747 GCTCTTC Tru9I 2845 TTAA T^TAA 2946 TTAA Van91I 1020 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1092 CCAGCTCCTGG XcmI 197 CCATGAAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1168 CCACTCCCCGCATGG XmnI 166 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2638 GAATGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AvaI AvaII BanI BanII BbvI BcgI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII XmaCI XmaI