##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AcyI        
AgeI        AhdI        Alw44I      ApaLI       ApoI        
AscI        AseI        AsnI        AspEI       AspI        
AvrII       BbeI        BbsI        BciVI       BclI        
BglII       BlnI        BpuAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BsrBI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        DraIII      EagI        Eam1105I    EclXI       
Eco52I      EcoRI       EcoRV       FbaI        FseI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfoI        
SfuI        SgrAI       SnaBI       SphI        Sse8387I    
SspI        SwaI        Tsp509I     Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

Acc65I        251  GGTACC               G^GTACC
AccI          688  GTCTAC               GT^[AC][GT]AC
AflII        2844  CTTAAG               C^TTAAG
AflIII       2812  ACATGT               A^C[GA][CT]GT
Aor51HI       992  AGCGCT               AGC^GCT
Asp718I       251  GGTACC               G^GTACC
AviII         275  TGCGCA               TGC^GCA
BamHI        2177  GGATCC               G^GATCC
BbrPI         966  CACGTG               CAC^GTG
BbvCI         534  CCTCAGC              CCTCAGC(-5/-2)
BfrI         2844  CTTAAG               C^TTAAG
BsaAI         966  CACGTG               [CT]AC^GT[GA]
Bsp1407I      230  TGTACA               T^GTACA
BspLU11I     2812  ACATGT               A^CATGT
BsrDI         782  GCAATG               GCAATG(2/0)
BsrGI         230  TGTACA               T^GTACA
Eco47III      992  AGCGCT               AGC^GCT
EcoT22I      2867  ATGCAT               ATGCA^T
FspI          275  TGCGCA               TGC^GCA
HgaI           41  GCGTC                GACGC(5/10)
KpnI          251  GGTACC               GGTAC^C
NcoI         1554  CCATGG               C^CATGG
NsiI         2867  ATGCAT               ATGCA^T
PmaCI         966  CACGTG               CAC^GTG
PmlI          966  CACGTG               CAC^GTG
SfiI         1942  GGCCAAAAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         2556  ACTAGT               A^CTAGT
SspBI         230  TGTACA               T^GTACA
StuI         1499  AGGCCT               AGG^CCT
XbaI         2829  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AocI          574  CCTCAGG              CC^T[ACGT]AGG
             2374  CCTCAGG             
Asp700I       166  GAATACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2638  GAATGCCTTC          
AspHI         801  GAGCTC               G[AT]GC[AT]^C
             1105  GAGCTC              
BalI          666  TGGCCA               TGG^CCA
             1754  TGGCCA              
BlpI          798  GCTGAGC              GC^T[ACGT]AGC
             1571  GCTCAGC             
Bpu1102I      798  GCTGAGC              GC^T[ACGT]AGC
             1571  GCTCAGC             
BsaI          790  GAGACC               GGTCTC(1/5)
             1391  GGTCTC              
BsiHKAI       801  GAGCTC               G[AT]GC[AT]^C
             1105  GAGCTC              
BsmI         1744  GAATGC               GAATGC(1/-1)
             2638  GAATGC              
BspHI         855  TCATGA               T^CATGA
             2491  TCATGA              
BsrFI         539  GCCGGC               [GA]^CCGG[CT]
             1112  GCCGGC              
BstEII        471  GGTCACC              G^GT[ACGT]ACC
             2454  GGTGACC             
BstPI         471  GGTCACC              G^GT[ACGT]ACC
             2454  GGTGACC             
BstXI        1617  CCACTCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2189  CCATCCGGCTGG        
Bsu36I        574  CCTCAGG              CC^T[ACGT]AGG
             2374  CCTCAGG             
CelII         798  GCTGAGC              GC^T[ACGT]AGC
             1571  GCTCAGC             
Cfr10I        539  GCCGGC               [GA]^CCGG[CT]
             1112  GCCGGC              
DrdI         1636  GACTCTGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1841  GACACTGGAGTC        
DsaI         1554  CCATGG               C^C[GA][CT]GG
             1978  CCACGG              
Eco81I        574  CCTCAGG              CC^T[ACGT]AGG
             2374  CCTCAGG             
EcoO65I       471  GGTCACC              G^GT[ACGT]ACC
             2454  GGTGACC             
MaeII         967  ACGT                 A^CGT
             2216  ACGT                
MluNI         666  TGGCCA               TGG^CCA
             1754  TGGCCA              
MscI          666  TGGCCA               TGG^CCA
             1754  TGGCCA              
MseI         2845  TTAA                 T^TAA
             2946  TTAA                
NaeI          539  GCCGGC               GCC^GGC
             1112  GCCGGC              
NgoMIV        539  GCCGGC               G^CCGGC
             1112  GCCGGC              
PflMI        1020  CCACTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1092  CCAGCTCCTGG         
RcaI          855  TCATGA               T^CATGA
             2491  TCATGA              
SacI          801  GAGCTC               GAGCT^C
             1105  GAGCTC              
SapI         1102  GAAGAGC              GCTCTTC(1/4)
              747  GCTCTTC             
Tru9I        2845  TTAA                 T^TAA
             2946  TTAA                
Van91I       1020  CCACTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1092  CCAGCTCCTGG         
XcmI          197  CCATGAAAGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1168  CCACTCCCCGCATGG     
XmnI          166  GAATACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2638  GAATGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       ApaI        AvaI        AvaII       BanI        
BanII       BbvI        BcgI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        StyI        TaqI        
TfiI        TseI        Tsp45I      TspRI       TthHB8I     
XhoII       XmaCI       XmaI