##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccII AclI AflIII AlwNI Aor51HI
AscI AseI AsnI Asp700I AspI
AviII AvrII BamHI BlnI BsaBI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrGI BssHII BstAPI BstBI
BstEII BstPI BstUI ClaI CpoI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI FspI HindIII HpaI
KspI MamI MluI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 4021 GACGTC GACGT^C
AccIII 1519 TCCGGA T^CCGGA
AcsI 4225 GAATTC [GA]^AATT[CT]
AgeI 241 ACCGGT A^CCGGT
AhdI 121 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 173 GTGCAC G^TGCAC
ApaLI 173 GTGCAC G^TGCAC
ApoI 4225 GAATTC [GA]^AATT[CT]
AspEI 121 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2961 GGCGCC GGCGC^C
BbrPI 2050 CACGTG CAC^GTG
BcgI 3397 CGACCTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 734 GCCACTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 1519 TCCGGA T^CCGGA
BspEI 1519 TCCGGA T^CCGGA
BspHI 2089 TCATGA T^CATGA
BsrFI 241 ACCGGT [GA]^CCGG[CT]
CfoI 2962 GCGC GCG^C
Cfr10I 241 ACCGGT [GA]^CCGG[CT]
DraI 271 TTTAAA TTT^AAA
DrdI 1040 GACAAATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 98 CCATGG C^C[GA][CT]GG
Eam1105I 121 GACTCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4225 GAATTC G^AATTC
EcoRV 5161 GATATC GAT^ATC
FauI 410 CCCGC CCCGC(4/6)
HaeII 2961 GGCGCC [GA]GCGC^[CT]
HgaI 2296 GCGTC GACGC(5/10)
HhaI 2962 GCGC GCG^C
HinP1I 2962 GCGC G^CGC
KasI 2961 GGCGCC G^GCGCC
MfeI 4775 CAATTG C^AATTG
MroI 1519 TCCGGA T^CCGGA
MunI 4775 CAATTG C^AATTG
NarI 2961 GGCGCC GG^CGCC
NcoI 98 CCATGG C^CATGG
NspI 688 GCATGC [GA]CATG^[CT]
PinAI 241 ACCGGT A^CCGGT
PmaCI 2050 CACGTG CAC^GTG
PmlI 2050 CACGTG CAC^GTG
RcaI 2089 TCATGA T^CATGA
ScaI 2899 AGTACT AGT^ACT
SfoI 2961 GGCGCC GGC^GCC
SmaI 1606 CCCGGG CCC^GGG
SphI 688 GCATGC GCATG^C
XbaI 943 TCTAGA T^CTAGA
XmaCI 1606 CCCGGG C^CCGGG
XmaI 1606 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 1748 GGTACC G^GTACC
3051 GGTACC
AcyI 2961 GGCGCC G[GA]^CG[CT]C
4021 GACGTC
AflII 785 CTTAAG C^TTAAG
2184 CTTAAG
Asp718I 1748 GGTACC G^GTACC
3051 GGTACC
BciVI 1356 GGATAC GTATCC(6/5)
230 GTATCC
BfrI 785 CTTAAG C^TTAAG
2184 CTTAAG
BsaAI 2050 CACGTG [CT]AC^GT[GA]
2073 TACGTG
BsaHI 2961 GGCGCC G[GA]^CG[CT]C
4021 GACGTC
BsmI 4442 GCATTC GAATGC(1/-1)
4478 GCATTC
BssSI 176 CACGAG CACGAG(-5/-1)
489 CACGAG
Bst1107I 1970 GTATAC GTA^TAC
2036 GTATAC
BstZ17I 1970 GTATAC GTA^TAC
2036 GTATAC
Hin1I 2961 GGCGCC G[GA]^CG[CT]C
4021 GACGTC
HincII 245 GTTGAC GT[CT]^[GA]AC
4986 GTTGAC
HindII 245 GTTGAC GT[CT]^[GA]AC
4986 GTTGAC
KpnI 1748 GGTACC GGTAC^C
3051 GGTACC
MspA1I 4364 CAGCTG C[AC]G^C[GT]G
4854 CAGCTG
PaeR7I 2 CTCGAG C^TCGAG
4211 CTCGAG
PstI 1151 CTGCAG CTGCA^G
2937 CTGCAG
PvuII 4364 CAGCTG CAG^CTG
4854 CAGCTG
XhoI 2 CTCGAG C^TCGAG
4211 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BclI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII Cfr13I
DdeI DpnII DraII DraIII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI RsaI SacI SapI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII