##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI AspI BamHI
BbeI BbrPI BcgI BciVI BcnI
BfrI BglII BsaBI BsaI BseAI
BsiWI BspDI BspEI BsrFI BssHII
Cfr10I ClaI CpoI DraIII EagI
EclXI Eco52I EcoRI FseI HpaI
KasI KspI MamI MfeI MluI
MroI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I RsrII
SacII SalI SfiI SfoI SgrAI
SmaI SnaBI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 4460 GGTACC G^GTACC
AccI 441 GTATAC GT^[AC][GT]AC
AccII 583 CGCG CG^CG
AcyI 752 GACGCC G[GA]^CG[CT]C
AhdI 2799 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2399 GTGCAC G^TGCAC
Aor51HI 611 AGCGCT AGC^GCT
ApaLI 2399 GTGCAC G^TGCAC
Asp700I 4513 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4460 GGTACC G^GTACC
AspEI 2799 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 713 TGCGCA TGC^GCA
AvrII 2729 CCTAGG C^CTAGG
BbsI 3082 GTCTTC GAAGAC(2/6)
BlnI 2729 CCTAGG C^CTAGG
BlpI 653 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 653 GCTCAGC GC^T[ACGT]AGC
BpuAI 3082 GTCTTC GAAGAC(2/6)
BsaAI 4322 TACGTG [CT]AC^GT[GA]
BsaHI 752 GACGCC G[GA]^CG[CT]C
BsiEI 126 CGATCG CG[GA][CT]^CG
BsmI 2818 GCATTC GAATGC(1/-1)
Bsp1407I 4357 TGTACA T^GTACA
BsrDI 470 GCAATG GCAATG(2/0)
BsrGI 4357 TGTACA T^GTACA
BssSI 1058 CTCGTG CACGAG(-5/-1)
Bst1107I 441 GTATAC GTA^TAC
BstBI 1416 TTCGAA TT^CGAA
BstEII 138 GGTCACC G^GT[ACGT]ACC
BstPI 138 GGTCACC G^GT[ACGT]ACC
BstUI 583 CGCG CG^CG
BstXI 4569 CCATGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 441 GTATAC GTA^TAC
CelII 653 GCTCAGC GC^T[ACGT]AGC
DrdI 2682 GACACACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2799 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 705 CTCTTC CTCTTC(1/4)
Eco47III 611 AGCGCT AGC^GCT
EcoO65I 138 GGTCACC G^GT[ACGT]ACC
EcoRV 1360 GATATC GAT^ATC
EcoT22I 3635 ATGCAT ATGCA^T
FspI 713 TGCGCA TGC^GCA
Hin1I 752 GACGCC G[GA]^CG[CT]C
HindIII 4336 AAGCTT A^AGCTT
KpnI 4460 GGTACC GGTAC^C
Ksp632I 705 CTCTTC CTCTTC(1/4)
MvnI 583 CGCG CG^CG
NsiI 3635 ATGCAT ATGCA^T
NspV 1416 TTCGAA TT^CGAA
PpuMI 3939 AGGTCCT [GA]G^G[AT]CC[CT]
PvuI 126 CGATCG CGAT^CG
SapI 704 GCTCTTC GCTCTTC(1/4)
SbfI 1549 CCTGCAGG CCTGCA^GG
ScaI 2741 AGTACT AGT^ACT
SexAI 2433 ACCAGGT A^CC[AT]GGT
SfuI 1416 TTCGAA TT^CGAA
Sse8387I 1549 CCTGCAGG CCTGCA^GG
SspBI 4357 TGTACA T^GTACA
SwaI 2567 ATTTAAAT ATTT^AAAT
XbaI 3789 TCTAGA T^CTAGA
XmnI 4513 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 1132 CCTGAGG CC^T[ACGT]AGG
1204 CCTCAGG
ApaI 1558 GGGCCC GGGCC^C
3482 GGGCCC
AvaI 872 CTCGGG C^[CT]CG[GA]G
3151 CCCGAG
BalI 249 TGGCCA TGG^CCA
1908 TGGCCA
BbvCI 1296 CCTCAGC CCTCAGC(-5/-2)
2246 GCTGAGG
BglI 671 GCCAGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3532 GCCATCAGGGC
BsaWI 843 ACCGGA [AT]^CCGG[AT]
2351 ACCGGA
BsoBI 872 CTCGGG C^[CT]CG[GA]G
3151 CCCGAG
BspHI 2717 TCATGA T^CATGA
3615 TCATGA
BspLU11I 2363 ACATGT A^CATGT
3807 ACATGT
BstAPI 2033 GCAAAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2283 GCAGTTAATGC
BstYI 1329 AGATCC [GA]^GATC[CT]
1844 AGATCC
Bsu36I 1132 CCTGAGG CC^T[ACGT]AGG
1204 CCTCAGG
EaeI 249 TGGCCA [CT]^GGCC[GA]
1908 TGGCCA
Eco81I 1132 CCTGAGG CC^T[ACGT]AGG
1204 CCTCAGG
HapII 844 CCGG C^CGG
2352 CCGG
HincII 1938 GTTGAC GT[CT]^[GA]AC
3987 GTTGAC
HindII 1938 GTTGAC GT[CT]^[GA]AC
3987 GTTGAC
HpaII 844 CCGG C^CGG
2352 CCGG
MflI 1329 AGATCC [GA]^GATC[CT]
1844 AGATCC
MluNI 249 TGGCCA TGG^CCA
1908 TGGCCA
MscI 249 TGGCCA TGG^CCA
1908 TGGCCA
MspI 844 CCGG C^CGG
2352 CCGG
PflMI 1365 CCATATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1537 CCAGAGGCTGG
PshAI 82 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4036 GACCAGTGTC
RcaI 2717 TCATGA T^CATGA
3615 TCATGA
SmlI 986 CTCAAG C^T[CT][GA]AG
4598 CTTGAG
SpeI 1078 ACTAGT A^CTAGT
1492 ACTAGT
SphI 319 GCATGC GCATG^C
605 GCATGC
SspI 1477 AATATT AAT^ATT
3391 AATATT
Van91I 1365 CCATATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1537 CCAGAGGCTGG
XhoII 1329 AGATCC [GA]^GATC[CT]
1844 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbvI BclI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HgaI HhaI HinP1I HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI