##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

Acc65I      AccIII      AgeI        Aor51HI     ApaI        
AscI        Asp718I     AspI        AviII       AvrII       
BciVI       BlnI        BlpI        Bpu1102I    BsaBI       
BsaWI       BseAI       BsiWI       BspDI       BspEI       
BspHI       BsrBI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     CelII       ClaI        CpoI        
Eco47III    EcoRV       FseI        FspI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NspV        PacI        PflFI       PinAI       PmeI        
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    Tth111I     


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII        6278  GACGTC               GACGT^C
AccI          637  GTCTAC               GT^[AC][GT]AC
AclI          474  AACGTT               AA^CGTT
AhdI         2260  GACCCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       6087  GTGCAC               G^TGCAC
ApaLI        6087  GTGCAC               G^TGCAC
AspEI        2260  GACCCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        5107  GGATCC               G^GATCC
BbeI         4347  GGCGCC               GGCGC^C
BbrPI        6034  CACGTG               CAC^GTG
BglI         3441  GCCGGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        6034  CACGTG               [CT]AC^GT[GA]
Bsp1407I     3201  TGTACA               T^GTACA
BspLU11I     5049  ACATGT               A^CATGT
BsrGI        3201  TGTACA               T^GTACA
BstEII        699  GGTCACC              G^GT[ACGT]ACC
BstPI         699  GGTCACC              G^GT[ACGT]ACC
DrdI         4271  GACACAGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1914  CGGCCG               C^GGCCG
Eam1105I     2260  GACCCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1914  CGGCCG               C^GGCCG
Eco52I       1914  CGGCCG               C^GGCCG
EcoNI         342  CCTTCTTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       699  GGTCACC              G^GT[ACGT]ACC
KasI         4347  GGCGCC               G^GCGCC
NarI         4347  GGCGCC               GG^CGCC
PaeR7I       3574  CTCGAG               C^TCGAG
PmaCI        6034  CACGTG               CAC^GTG
PmlI         6034  CACGTG               CAC^GTG
PshAI        6244  GACAGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      474  AACGTT               AA^CGTT
SacI         1850  GAGCTC               GAGCT^C
SapI         5894  GCTCTTC              GCTCTTC(1/4)
SexAI        2028  ACCAGGT              A^CC[AT]GGT
SfoI         4347  GGCGCC               GGC^GCC
SphI         4311  GCATGC               GCATG^C
SspBI        3201  TGTACA               T^GTACA
SwaI         5226  ATTTAAAT             ATTT^AAAT
XhoI         3574  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII          34  CGCG                 CG^CG
             3484  CGCG                
AflII         346  CTTAAG               C^TTAAG
             1934  CTTAAG              
AflIII       5049  ACATGT               A^C[GA][CT]GT
             6493  ACGTGT              
AocI         5548  CCTAAGG              CC^T[ACGT]AGG
             5906  CCTCAGG             
BclI          394  TGATCA               T^GATCA
             2764  TGATCA              
BfrI          346  CTTAAG               C^TTAAG
             1934  CTTAAG              
BglII        1132  AGATCT               A^GATCT
             2367  AGATCT              
BsiEI        1914  CGGCCG               CG[GA][CT]^CG
             3436  CGGTCG              
BsmBI        1897  CGTCTC               CGTCTC(1/5)
             5761  CGTCTC              
BstUI          34  CGCG                 CG^CG
             3484  CGCG                
Bsu36I       5548  CCTAAGG              CC^T[ACGT]AGG
             5906  CCTCAGG             
Eco81I       5548  CCTAAGG              CC^T[ACGT]AGG
             5906  CCTCAGG             
EcoRI        1310  GAATTC               G^AATTC
             2662  GAATTC              
EcoT22I      4562  ATGCAT               ATGCA^T
             5937  ATGCAT              
FbaI          394  TGATCA               T^GATCA
             2764  TGATCA              
HpaI         5045  GTTAAC               GTT^AAC
             5354  GTTAAC              
MvnI           34  CGCG                 CG^CG
             3484  CGCG                
NaeI         1736  GCCGGC               GCC^GGC
             3441  GCCGGC              
NcoI         2868  CCATGG               C^CATGG
             6345  CCATGG              
NgoMIV       1736  GCCGGC               G^CCGGC
             3441  GCCGGC              
NsiI         4562  ATGCAT               ATGCA^T
             5937  ATGCAT              
PpuMI        3271  GGGACCT              [GA]G^G[AT]CC[CT]
             5838  AGGACCC             
SmaI         3456  CCCGGG               CCC^GGG
             5689  CCCGGG              
XbaI         1550  TCTAGA               T^CTAGA
             2168  TCTAGA              
XmaCI        3456  CCCGGG               C^CCGGG
             5689  CCCGGG              
XmaI         3456  CCCGGG               C^CCGGG
             5689  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  130 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
Asp700I     AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvCI       BbvI        
BcgI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrDI       BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DraII       DraIII      DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PshBI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
SspI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       XmnI