################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## Acc65I AccIII AgeI Aor51HI ApaI AscI Asp718I AspI AviII AvrII BciVI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI Eco47III EcoRV FseI FspI KpnI KspI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I Tth111I ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 6278 GACGTC GACGT^C AccI 637 GTCTAC GT^[AC][GT]AC AclI 474 AACGTT AA^CGTT AhdI 2260 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 6087 GTGCAC G^TGCAC ApaLI 6087 GTGCAC G^TGCAC AspEI 2260 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 5107 GGATCC G^GATCC BbeI 4347 GGCGCC GGCGC^C BbrPI 6034 CACGTG CAC^GTG BglI 3441 GCCGGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 6034 CACGTG [CT]AC^GT[GA] Bsp1407I 3201 TGTACA T^GTACA BspLU11I 5049 ACATGT A^CATGT BsrGI 3201 TGTACA T^GTACA BstEII 699 GGTCACC G^GT[ACGT]ACC BstPI 699 GGTCACC G^GT[ACGT]ACC DrdI 4271 GACACAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1914 CGGCCG C^GGCCG Eam1105I 2260 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1914 CGGCCG C^GGCCG Eco52I 1914 CGGCCG C^GGCCG EcoNI 342 CCTTCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 699 GGTCACC G^GT[ACGT]ACC KasI 4347 GGCGCC G^GCGCC NarI 4347 GGCGCC GG^CGCC PaeR7I 3574 CTCGAG C^TCGAG PmaCI 6034 CACGTG CAC^GTG PmlI 6034 CACGTG CAC^GTG PshAI 6244 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 474 AACGTT AA^CGTT SacI 1850 GAGCTC GAGCT^C SapI 5894 GCTCTTC GCTCTTC(1/4) SexAI 2028 ACCAGGT A^CC[AT]GGT SfoI 4347 GGCGCC GGC^GCC SphI 4311 GCATGC GCATG^C SspBI 3201 TGTACA T^GTACA SwaI 5226 ATTTAAAT ATTT^AAAT XhoI 3574 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 34 CGCG CG^CG 3484 CGCG AflII 346 CTTAAG C^TTAAG 1934 CTTAAG AflIII 5049 ACATGT A^C[GA][CT]GT 6493 ACGTGT AocI 5548 CCTAAGG CC^T[ACGT]AGG 5906 CCTCAGG BclI 394 TGATCA T^GATCA 2764 TGATCA BfrI 346 CTTAAG C^TTAAG 1934 CTTAAG BglII 1132 AGATCT A^GATCT 2367 AGATCT BsiEI 1914 CGGCCG CG[GA][CT]^CG 3436 CGGTCG BsmBI 1897 CGTCTC CGTCTC(1/5) 5761 CGTCTC BstUI 34 CGCG CG^CG 3484 CGCG Bsu36I 5548 CCTAAGG CC^T[ACGT]AGG 5906 CCTCAGG Eco81I 5548 CCTAAGG CC^T[ACGT]AGG 5906 CCTCAGG EcoRI 1310 GAATTC G^AATTC 2662 GAATTC EcoT22I 4562 ATGCAT ATGCA^T 5937 ATGCAT FbaI 394 TGATCA T^GATCA 2764 TGATCA HpaI 5045 GTTAAC GTT^AAC 5354 GTTAAC MvnI 34 CGCG CG^CG 3484 CGCG NaeI 1736 GCCGGC GCC^GGC 3441 GCCGGC NcoI 2868 CCATGG C^CATGG 6345 CCATGG NgoMIV 1736 GCCGGC G^CCGGC 3441 GCCGGC NsiI 4562 ATGCAT ATGCA^T 5937 ATGCAT PpuMI 3271 GGGACCT [GA]G^G[AT]CC[CT] 5838 AGGACCC SmaI 3456 CCCGGG CCC^GGG 5689 CCCGGG XbaI 1550 TCTAGA T^CTAGA 2168 TCTAGA XmaCI 3456 CCCGGG C^CCGGG 5689 CCCGGG XmaI 3456 CCCGGG C^CCGGG 5689 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI