##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccIII AgeI Aor51HI ApaI
AscI Asp718I AspI AviII AvrII
BciVI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BspHI BsrBI BssHII BssSI Bst1107I
BstBI BstZ17I CelII ClaI CpoI
Eco47III EcoRV FseI FspI KpnI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I Tth111I
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 6278 GACGTC GACGT^C
AccI 637 GTCTAC GT^[AC][GT]AC
AclI 474 AACGTT AA^CGTT
AhdI 2260 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 6087 GTGCAC G^TGCAC
ApaLI 6087 GTGCAC G^TGCAC
AspEI 2260 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 5107 GGATCC G^GATCC
BbeI 4347 GGCGCC GGCGC^C
BbrPI 6034 CACGTG CAC^GTG
BglI 3441 GCCGGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 6034 CACGTG [CT]AC^GT[GA]
Bsp1407I 3201 TGTACA T^GTACA
BspLU11I 5049 ACATGT A^CATGT
BsrGI 3201 TGTACA T^GTACA
BstEII 699 GGTCACC G^GT[ACGT]ACC
BstPI 699 GGTCACC G^GT[ACGT]ACC
DrdI 4271 GACACAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1914 CGGCCG C^GGCCG
Eam1105I 2260 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1914 CGGCCG C^GGCCG
Eco52I 1914 CGGCCG C^GGCCG
EcoNI 342 CCTTCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 699 GGTCACC G^GT[ACGT]ACC
KasI 4347 GGCGCC G^GCGCC
NarI 4347 GGCGCC GG^CGCC
PaeR7I 3574 CTCGAG C^TCGAG
PmaCI 6034 CACGTG CAC^GTG
PmlI 6034 CACGTG CAC^GTG
PshAI 6244 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 474 AACGTT AA^CGTT
SacI 1850 GAGCTC GAGCT^C
SapI 5894 GCTCTTC GCTCTTC(1/4)
SexAI 2028 ACCAGGT A^CC[AT]GGT
SfoI 4347 GGCGCC GGC^GCC
SphI 4311 GCATGC GCATG^C
SspBI 3201 TGTACA T^GTACA
SwaI 5226 ATTTAAAT ATTT^AAAT
XhoI 3574 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 34 CGCG CG^CG
3484 CGCG
AflII 346 CTTAAG C^TTAAG
1934 CTTAAG
AflIII 5049 ACATGT A^C[GA][CT]GT
6493 ACGTGT
AocI 5548 CCTAAGG CC^T[ACGT]AGG
5906 CCTCAGG
BclI 394 TGATCA T^GATCA
2764 TGATCA
BfrI 346 CTTAAG C^TTAAG
1934 CTTAAG
BglII 1132 AGATCT A^GATCT
2367 AGATCT
BsiEI 1914 CGGCCG CG[GA][CT]^CG
3436 CGGTCG
BsmBI 1897 CGTCTC CGTCTC(1/5)
5761 CGTCTC
BstUI 34 CGCG CG^CG
3484 CGCG
Bsu36I 5548 CCTAAGG CC^T[ACGT]AGG
5906 CCTCAGG
Eco81I 5548 CCTAAGG CC^T[ACGT]AGG
5906 CCTCAGG
EcoRI 1310 GAATTC G^AATTC
2662 GAATTC
EcoT22I 4562 ATGCAT ATGCA^T
5937 ATGCAT
FbaI 394 TGATCA T^GATCA
2764 TGATCA
HpaI 5045 GTTAAC GTT^AAC
5354 GTTAAC
MvnI 34 CGCG CG^CG
3484 CGCG
NaeI 1736 GCCGGC GCC^GGC
3441 GCCGGC
NcoI 2868 CCATGG C^CATGG
6345 CCATGG
NgoMIV 1736 GCCGGC G^CCGGC
3441 GCCGGC
NsiI 4562 ATGCAT ATGCA^T
5937 ATGCAT
PpuMI 3271 GGGACCT [GA]G^G[AT]CC[CT]
5838 AGGACCC
SmaI 3456 CCCGGG CCC^GGG
5689 CCCGGG
XbaI 1550 TCTAGA T^CTAGA
2168 TCTAGA
XmaCI 3456 CCCGGG C^CCGGG
5689 CCCGGG
XmaI 3456 CCCGGG C^CCGGG
5689 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI