##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
AviII BamHI BcgI BciVI BfrI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI Bsp1407I BspEI BspHI BsrGI
BstBI BstEII BstPI CelII CpoI
Eam1105I EcoO65I EcoRV FseI FspI
HpaI MamI MfeI MroI MunI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SfiI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 3777 GTATAC GT^[AC][GT]AC
Aor51HI 486 AGCGCT AGC^GCT
Asp700I 6171 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 4755 CACGTG CAC^GTG
BclI 4312 TGATCA T^GATCA
BglII 2816 AGATCT A^GATCT
BsmBI 38 GAGACG CGTCTC(1/5)
BspDI 4618 ATCGAT AT^CGAT
BsrFI 236 GCCGGC [GA]^CCGG[CT]
Bst1107I 3777 GTATAC GTA^TAC
BstZ17I 3777 GTATAC GTA^TAC
Cfr10I 236 GCCGGC [GA]^CCGG[CT]
ClaI 4618 ATCGAT AT^CGAT
DraI 5575 TTTAAA TTT^AAA
EagI 234 CGGCCG C^GGCCG
EclXI 234 CGGCCG C^GGCCG
Eco47III 486 AGCGCT AGC^GCT
Eco52I 234 CGGCCG C^GGCCG
EcoRI 5626 GAATTC G^AATTC
EcoT22I 2778 ATGCAT ATGCA^T
FbaI 4312 TGATCA T^GATCA
HindIII 5786 AAGCTT A^AGCTT
KspI 586 CCGCGG CCGC^GG
MluI 41 ACGCGT A^CGCGT
NaeI 236 GCCGGC GCC^GGC
NdeI 6445 CATATG CA^TATG
NgoMIV 236 GCCGGC G^CCGGC
NsiI 2778 ATGCAT ATGCA^T
PaeR7I 5195 CTCGAG C^TCGAG
PmaCI 4755 CACGTG CAC^GTG
PmlI 4755 CACGTG CAC^GTG
SacII 586 CCGCGG CCGC^GG
SbfI 1717 CCTGCAGG CCTGCA^GG
ScaI 906 AGTACT AGT^ACT
SphI 1509 GCATGC GCATG^C
Sse8387I 1717 CCTGCAGG CCTGCA^GG
XbaI 4971 TCTAGA T^CTAGA
XhoI 5195 CTCGAG C^TCGAG
XmnI 6171 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 4419 GGTACC G^GTACC
4715 GGTACC
AcsI 5578 AAATTT [GA]^AATT[CT]
5626 GAATTC
ApoI 5578 AAATTT [GA]^AATT[CT]
5626 GAATTC
Asp718I 4419 GGTACC G^GTACC
4715 GGTACC
BbeI 25 GGCGCC GGCGC^C
728 GGCGCC
BsaAI 1063 TACGTG [CT]AC^GT[GA]
4755 CACGTG
BsiEI 234 CGGCCG CG[GA][CT]^CG
1069 CGACCG
BsmI 202 GCATTC GAATGC(1/-1)
2115 GCATTC
BspLU11I 192 ACATGT A^CATGT
6515 ACATGT
BstAPI 1183 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1639 GCAGGCTGTGC
DraIII 1169 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2339 CACTCAGTG
DrdI 2452 GACTTCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5030 GACTACCTCGTC
HincII 3350 GTTGAC GT[CT]^[GA]AC
3819 GTTGAC
HindII 3350 GTTGAC GT[CT]^[GA]AC
3819 GTTGAC
KasI 25 GGCGCC G^GCGCC
728 GGCGCC
KpnI 4419 GGTACC GGTAC^C
4715 GGTACC
NarI 25 GGCGCC GG^CGCC
728 GGCGCC
NheI 2354 GCTAGC G^CTAGC
5753 GCTAGC
PshAI 4049 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4909 GACTCCTGTC
SacI 1028 GAGCTC GAGCT^C
1037 GAGCTC
SapI 382 GCTCTTC GCTCTTC(1/4)
1899 GCTCTTC
SfoI 25 GGCGCC GGC^GCC
728 GGCGCC
SmaI 1734 CCCGGG CCC^GGG
5631 CCCGGG
XmaCI 1734 CCCGGG C^CCGGG
5631 CCCGGG
XmaI 1734 CCCGGG C^CCGGG
5631 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AspI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlnI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssHII BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII