##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       Acc65I      AccII       AgeI        Alw44I      
AocI        ApaI        ApaLI       AscI        Asp718I     
AspI        AvrII       BbeI        BbrPI       BcgI        
BglI        BlnI        BsiWI       BspDI       BspMI       
BsrBI       BsrFI       BssHII      BstBI       BstEII      
BstPI       BstUI       Bsu36I      Cfr10I      ClaI        
CpoI        EagI        EclXI       Eco52I      Eco81I      
EcoO65I     EcoRV       FauI        FseI        HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       Sse8387I    SwaI        Tth111I     
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccIII        272  TCCGGA               T^CCGGA
AclI         1501  AACGTT               AA^CGTT
AcyI         1255  GACGCC               G[GA]^CG[CT]C
AflIII       5328  ACATGT               A^C[GA][CT]GT
AhdI         5115  GACTAACCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3459  AGCGCT               AGC^GCT
AseI         5918  ATTAAT               AT^TAAT
AsnI         5918  ATTAAT               AT^TAAT
AspEI        5115  GACTAACCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3067  TGCGCA               TGC^GCA
BalI         4289  TGGCCA               TGG^CCA
BamHI        2405  GGATCC               G^GATCC
BanI         1034  GGCACC               G^G[CT][GA]CC
BciVI        3141  GTATCC               GTATCC(6/5)
BsaHI        1255  GACGCC               G[GA]^CG[CT]C
BseAI         272  TCCGGA               T^CCGGA
BsiEI        3824  CGGTCG               CG[GA][CT]^CG
BsmBI        1682  GAGACG               CGTCTC(1/5)
BsmI         4349  GAATGC               GAATGC(1/-1)
BspEI         272  TCCGGA               T^CCGGA
BspLU11I     5328  ACATGT               A^CATGT
BssSI         109  CACGAG               CACGAG(-5/-1)
EaeI         4289  TGGCCA               [CT]^GGCC[GA]
Eam1105I     5115  GACTAACCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3459  AGCGCT               AGC^GCT
EcoRI         792  GAATTC               G^AATTC
FspI         3067  TGCGCA               TGC^GCA
Hin1I        1255  GACGCC               G[GA]^CG[CT]C
HincII       2612  GTCAAC               GT[CT]^[GA]AC
HindII       2612  GTCAAC               GT[CT]^[GA]AC
HindIII      3088  AAGCTT               A^AGCTT
MluNI        4289  TGGCCA               TGG^CCA
MroI          272  TCCGGA               T^CCGGA
MscI         4289  TGGCCA               TGG^CCA
NcoI          544  CCATGG               C^CATGG
PshBI        5918  ATTAAT               AT^TAAT
Psp1406I     1501  AACGTT               AA^CGTT
SacI          144  GAGCTC               GAGCT^C
SapI         4308  GCTCTTC              GCTCTTC(1/4)
SmaI         4268  CCCGGG               CCC^GGG
SnaBI        5020  TACGTA               TAC^GTA
SphI          881  GCATGC               GCATG^C
XmaCI        4268  CCCGGG               C^CCGGG
XmaI         4268  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AflII         381  CTTAAG               C^TTAAG
             1250  CTTAAG              
AspHI         144  GAGCTC               G[AT]GC[AT]^C
             3073  GAGCAC              
AvaI         3589  CTCGGG               C^[CT]CG[GA]G
             4268  CCCGGG              
BcnI         4268  CCCGG                CC^[GC]GG
             4269  CCGGG               
BfrI          381  CTTAAG               C^TTAAG
             1250  CTTAAG              
BlpI           42  GCTAAGC              GC^T[ACGT]AGC
             3210  GCTCAGC             
Bpu1102I       42  GCTAAGC              GC^T[ACGT]AGC
             3210  GCTCAGC             
BsaAI        2482  TACGTG               [CT]AC^GT[GA]
             5020  TACGTA              
BsaBI         327  GATACAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1338  GATGACAATC          
BsaI          937  GAGACC               GGTCTC(1/5)
             5245  GGTCTC              
BsiHKAI       144  GAGCTC               G[AT]GC[AT]^C
             3073  GAGCAC              
BsoBI        3589  CTCGGG               C^[CT]CG[GA]G
             4268  CCCGGG              
BspHI         830  TCATGA               T^CATGA
             5396  TCATGA              
Bst1107I     1815  GTATAC               GTA^TAC
             4375  GTATAC              
BstXI         665  CCATGAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2746  CCACAGATGTGG        
BstZ17I      1815  GTATAC               GTA^TAC
             4375  GTATAC              
CelII          42  GCTAAGC              GC^T[ACGT]AGC
             3210  GCTCAGC             
DraII         364  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             5544  AGGACCC             
DraIII       2167  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4966  CACTCTGTG           
DrdI         3390  GACTCCAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4521  GACAGCTTGGTC        
EcoO109I      364  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             5544  AGGACCC             
EcoT22I      3732  ATGCAT               ATGCA^T
             6412  ATGCAT              
MamI          327  GATACAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1338  GATGACAATC          
NciI         4268  CCCGG                CC^[GC]GG
             4269  CCGGG               
NheI         1061  GCTAGC               G^CTAGC
             1662  GCTAGC              
NsiI         3732  ATGCAT               ATGCA^T
             6412  ATGCAT              
PflMI         576  CCATTATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4281  CCAACACTTGG         
PpuMI         364  GGGTCCC              [GA]G^G[AT]CC[CT]
             5544  AGGACCC             
PstI          721  CTGCAG               CTGCA^G
             1417  CTGCAG              
RcaI          830  TCATGA               T^CATGA
             5396  TCATGA              
SpeI         3329  ACTAGT               A^CTAGT
             3480  ACTAGT              
StuI         1359  AGGCCT               AGG^CCT
             5567  AGGCCT              
Van91I        576  CCATTATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4281  CCAACACTTGG         
XbaI         2468  TCTAGA               T^CTAGA
             3273  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  103 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AvaII       
BanII       BbsI        BbvCI       BbvI        BclI        
BfaI        BglII       BmyI        BpmI        BpuAI       
BsaJI       BsaWI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    Bsp1407I    
BsrDI       BsrGI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DsaI        
EarI        EcoNI       EcoRII      EcoT14I     FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       SfcI        SmlI        
SspBI       SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI