##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccII AgeI Alw44I
AocI ApaI ApaLI AscI Asp718I
AspI AvrII BbeI BbrPI BcgI
BglI BlnI BsiWI BspDI BspMI
BsrBI BsrFI BssHII BstBI BstEII
BstPI BstUI Bsu36I Cfr10I ClaI
CpoI EagI EclXI Eco52I Eco81I
EcoO65I EcoRV FauI FseI HpaI
KasI KpnI KspI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI Sse8387I SwaI Tth111I
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 272 TCCGGA T^CCGGA
AclI 1501 AACGTT AA^CGTT
AcyI 1255 GACGCC G[GA]^CG[CT]C
AflIII 5328 ACATGT A^C[GA][CT]GT
AhdI 5115 GACTAACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3459 AGCGCT AGC^GCT
AseI 5918 ATTAAT AT^TAAT
AsnI 5918 ATTAAT AT^TAAT
AspEI 5115 GACTAACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3067 TGCGCA TGC^GCA
BalI 4289 TGGCCA TGG^CCA
BamHI 2405 GGATCC G^GATCC
BanI 1034 GGCACC G^G[CT][GA]CC
BciVI 3141 GTATCC GTATCC(6/5)
BsaHI 1255 GACGCC G[GA]^CG[CT]C
BseAI 272 TCCGGA T^CCGGA
BsiEI 3824 CGGTCG CG[GA][CT]^CG
BsmBI 1682 GAGACG CGTCTC(1/5)
BsmI 4349 GAATGC GAATGC(1/-1)
BspEI 272 TCCGGA T^CCGGA
BspLU11I 5328 ACATGT A^CATGT
BssSI 109 CACGAG CACGAG(-5/-1)
EaeI 4289 TGGCCA [CT]^GGCC[GA]
Eam1105I 5115 GACTAACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3459 AGCGCT AGC^GCT
EcoRI 792 GAATTC G^AATTC
FspI 3067 TGCGCA TGC^GCA
Hin1I 1255 GACGCC G[GA]^CG[CT]C
HincII 2612 GTCAAC GT[CT]^[GA]AC
HindII 2612 GTCAAC GT[CT]^[GA]AC
HindIII 3088 AAGCTT A^AGCTT
MluNI 4289 TGGCCA TGG^CCA
MroI 272 TCCGGA T^CCGGA
MscI 4289 TGGCCA TGG^CCA
NcoI 544 CCATGG C^CATGG
PshBI 5918 ATTAAT AT^TAAT
Psp1406I 1501 AACGTT AA^CGTT
SacI 144 GAGCTC GAGCT^C
SapI 4308 GCTCTTC GCTCTTC(1/4)
SmaI 4268 CCCGGG CCC^GGG
SnaBI 5020 TACGTA TAC^GTA
SphI 881 GCATGC GCATG^C
XmaCI 4268 CCCGGG C^CCGGG
XmaI 4268 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflII 381 CTTAAG C^TTAAG
1250 CTTAAG
AspHI 144 GAGCTC G[AT]GC[AT]^C
3073 GAGCAC
AvaI 3589 CTCGGG C^[CT]CG[GA]G
4268 CCCGGG
BcnI 4268 CCCGG CC^[GC]GG
4269 CCGGG
BfrI 381 CTTAAG C^TTAAG
1250 CTTAAG
BlpI 42 GCTAAGC GC^T[ACGT]AGC
3210 GCTCAGC
Bpu1102I 42 GCTAAGC GC^T[ACGT]AGC
3210 GCTCAGC
BsaAI 2482 TACGTG [CT]AC^GT[GA]
5020 TACGTA
BsaBI 327 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1338 GATGACAATC
BsaI 937 GAGACC GGTCTC(1/5)
5245 GGTCTC
BsiHKAI 144 GAGCTC G[AT]GC[AT]^C
3073 GAGCAC
BsoBI 3589 CTCGGG C^[CT]CG[GA]G
4268 CCCGGG
BspHI 830 TCATGA T^CATGA
5396 TCATGA
Bst1107I 1815 GTATAC GTA^TAC
4375 GTATAC
BstXI 665 CCATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2746 CCACAGATGTGG
BstZ17I 1815 GTATAC GTA^TAC
4375 GTATAC
CelII 42 GCTAAGC GC^T[ACGT]AGC
3210 GCTCAGC
DraII 364 GGGTCCC [GA]G^G[ACGT]CC[CT]
5544 AGGACCC
DraIII 2167 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
4966 CACTCTGTG
DrdI 3390 GACTCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4521 GACAGCTTGGTC
EcoO109I 364 GGGTCCC [GA]G^G[ACGT]CC[CT]
5544 AGGACCC
EcoT22I 3732 ATGCAT ATGCA^T
6412 ATGCAT
MamI 327 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1338 GATGACAATC
NciI 4268 CCCGG CC^[GC]GG
4269 CCGGG
NheI 1061 GCTAGC G^CTAGC
1662 GCTAGC
NsiI 3732 ATGCAT ATGCA^T
6412 ATGCAT
PflMI 576 CCATTATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4281 CCAACACTTGG
PpuMI 364 GGGTCCC [GA]G^G[AT]CC[CT]
5544 AGGACCC
PstI 721 CTGCAG CTGCA^G
1417 CTGCAG
RcaI 830 TCATGA T^CATGA
5396 TCATGA
SpeI 3329 ACTAGT A^CTAGT
3480 ACTAGT
StuI 1359 AGGCCT AGG^CCT
5567 AGGCCT
Van91I 576 CCATTATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4281 CCAACACTTGG
XbaI 2468 TCTAGA T^CTAGA
3273 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BanII BbsI BbvCI BbvI BclI
BfaI BglII BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EarI EcoNI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI