##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI AscI Asp700I
AspEI AvaI AviII BalI BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlpI Bpu1102I BsaAI BsaHI
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I CelII ClaI CpoI
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV FbaI
FseI FspI HgaI Hin1I KasI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I StuI
SwaI XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 3533 GGTACC G^GTACC
Alw44I 3087 GTGCAC G^TGCAC
Aor51HI 2882 AGCGCT AGC^GCT
ApaLI 3087 GTGCAC G^TGCAC
AseI 2848 ATTAAT AT^TAAT
AsnI 2848 ATTAAT AT^TAAT
Asp718I 3533 GGTACC G^GTACC
AspI 280 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4922 CCTAGG C^CTAGG
BamHI 4475 GGATCC G^GATCC
BlnI 4922 CCTAGG C^CTAGG
BsaBI 2943 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2084 ACCGGA [AT]^CCGG[AT]
BspLU11I 493 ACATGT A^CATGT
BspMI 3056 ACCTGC ACCTGC(4/8)
BsrFI 1096 ACCGGC [GA]^CCGG[CT]
Cfr10I 1096 ACCGGC [GA]^CCGG[CT]
DraIII 2962 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2882 AGCGCT AGC^GCT
EcoT22I 1108 ATGCAT ATGCA^T
HpaI 4262 GTTAAC GTT^AAC
KpnI 3533 GGTACC GGTAC^C
MamI 2943 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 1108 ATGCAT ATGCA^T
PflFI 280 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2848 ATTAAT AT^TAAT
SfiI 3358 GGCCCTACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 4587 CTTGAG C^T[CT][GA]AG
SpeI 4135 ACTAGT A^CTAGT
SphI 1576 GCATGC GCATG^C
SspI 3134 AATATT AAT^ATT
Tth111I 280 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 493 ACATGT A^C[GA][CT]GT
509 ACGTGT
ApaI 409 GGGCCC GGGCC^C
551 GGGCCC
BcnI 1465 CCCGG CC^[GC]GG
3522 CCGGG
BsaI 1218 GAGACC GGTCTC(1/5)
4239 GGTCTC
BsmBI 3814 CGTCTC CGTCTC(1/5)
4324 CGTCTC
BsmI 332 GAATGC GAATGC(1/-1)
2073 GAATGC
BspHI 1225 TCATGA T^CATGA
3789 TCATGA
BstAPI 1576 GCATGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2733 GCACCAGCTGC
BstEII 4148 GGTGACC G^GT[ACGT]ACC
4758 GGTTACC
BstPI 4148 GGTGACC G^GT[ACGT]ACC
4758 GGTTACC
BstXI 3701 CCACAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4688 CCACATTTTTGG
EcoO65I 4148 GGTGACC G^GT[ACGT]ACC
4758 GGTTACC
HaeII 738 AGCGCC [GA]GCGC^[CT]
2882 AGCGCT
HincII 1371 GTCAAC GT[CT]^[GA]AC
4262 GTTAAC
HindII 1371 GTCAAC GT[CT]^[GA]AC
4262 GTTAAC
HindIII 390 AAGCTT A^AGCTT
3713 AAGCTT
MaeII 509 ACGT A^CGT
3813 ACGT
NciI 1465 CCCGG CC^[GC]GG
3522 CCGGG
PstI 769 CTGCAG CTGCA^G
2740 CTGCAG
RcaI 1225 TCATGA T^CATGA
3789 TCATGA
SapI 783 GCTCTTC GCTCTTC(1/4)
3034 GCTCTTC
TaqI 4039 TCGA T^CGA
4622 TCGA
TthHB8I 4039 TCGA T^CGA
4622 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BfaI BglI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BsrBI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII