##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      AocI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BfrI        BsaAI       BsaBI       BsiWI       BsmBI       
BspDI       BspHI       BssHII      Bst1107I    BstBI       
BstZ17I     Bsu36I      ClaI        DraI        DrdI        
Eam1105I    Eco81I      EcoRI       EcoRV       FseI        
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MunI        NaeI        NgoMIV      NheI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        SalI        
ScaI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SspI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AflIII       1721  ACATGT               A^C[GA][CT]GT
AlwI         2441  GGATC                GGATC(4/5)
Aor51HI       662  AGCGCT               AGC^GCT
ApaI          763  GGGCCC               GGGCC^C
Asp700I       561  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        2844  TGCGCA               TGC^GCA
AvrII         378  CCTAGG               C^CTAGG
BalI         1064  TGGCCA               TGG^CCA
BbeI         1149  GGCGCC               GGCGC^C
BclI         1523  TGATCA               T^GATCA
BglII        1109  AGATCT               A^GATCT
BlnI          378  CCTAGG               C^CTAGG
BlpI          646  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      646  GCTGAGC              GC^T[ACGT]AGC
Bsp1407I     1733  TGTACA               T^GTACA
BspLU11I     1721  ACATGT               A^CATGT
BsrFI        1493  ACCGGC               [GA]^CCGG[CT]
BsrGI        1733  TGTACA               T^GTACA
BstEII       2304  GGTGACC              G^GT[ACGT]ACC
BstPI        2304  GGTGACC              G^GT[ACGT]ACC
BstYI        1109  AGATCT               [GA]^GATC[CT]
CelII         646  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1493  ACCGGC               [GA]^CCGG[CT]
Eco47III      662  AGCGCT               AGC^GCT
EcoO65I      2304  GGTGACC              G^GT[ACGT]ACC
EcoT22I      3218  ATGCAT               ATGCA^T
FbaI         1523  TGATCA               T^GATCA
FspI         2844  TGCGCA               TGC^GCA
HincII       1947  GTTGAC               GT[CT]^[GA]AC
HindII       1947  GTTGAC               GT[CT]^[GA]AC
KasI         1149  GGCGCC               G^GCGCC
MflI         1109  AGATCT               [GA]^GATC[CT]
MluNI        1064  TGGCCA               TGG^CCA
MscI         1064  TGGCCA               TGG^CCA
MseI          931  TTAA                 T^TAA
NarI         1149  GGCGCC               GG^CGCC
NdeI         3032  CATATG               CA^TATG
NotI            1  GCGGCCGC             GC^GGCCGC
NsiI         3218  ATGCAT               ATGCA^T
SapI         2235  GCTCTTC              GCTCTTC(1/4)
SbfI         1869  CCTGCAGG             CCTGCA^GG
SfoI         1149  GGCGCC               GGC^GCC
SmaI           83  CCCGGG               CCC^GGG
SphI         1953  GCATGC               GCATG^C
Sse8387I     1869  CCTGCAGG             CCTGCA^GG
SspBI        1733  TGTACA               T^GTACA
TaqI          438  TCGA                 T^CGA
Tru9I         931  TTAA                 T^TAA
TthHB8I       438  TCGA                 T^CGA
XhoII        1109  AGATCT               [GA]^GATC[CT]
XmaCI          83  CCCGGG               C^CCGGG
XmaI           83  CCCGGG               C^CCGGG
XmnI          561  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          841  GTCTAC               GT^[AC][GT]AC
             2433  GTAGAC              
AccIII       1535  TCCGGA               T^CCGGA
             2036  TCCGGA              
AcsI         1694  AAATTT               [GA]^AATT[CT]
             1728  GAATTT              
ApoI         1694  AAATTT               [GA]^AATT[CT]
             1728  GAATTT              
BsaWI        1535  TCCGGA               [AT]^CCGG[AT]
             2036  TCCGGA              
BseAI        1535  TCCGGA               T^CCGGA
             2036  TCCGGA              
BsgI          350  CTGCAC               GTGCAG(16/14)
             1358  CTGCAC              
BsmI         1818  GAATGC               GAATGC(1/-1)
              434  GCATTC              
BspEI        1535  TCCGGA               T^CCGGA
             2036  TCCGGA              
BsrBI          13  GAGCGG               CCGCTC(-3/-3)
               88  GAGCGG              
BsrDI        2127  CATTGC               GCAATG(2/0)
              875  GCAATG              
BssSI        1020  CACGAG               CACGAG(-5/-1)
             2293  CACGAG              
BstAPI       1035  GCAGCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2199  GCAGCAACTGC         
CpoI         1537  CGGACCG              CG^G[AT]CCG
             2270  CGGTCCG             
HaeII         662  AGCGCT               [GA]GCGC^[CT]
             1149  GGCGCC              
KspI            5  CCGCGG               CCGC^GG
               77  CCGCGG              
MaeII         143  ACGT                 A^CGT
             1486  ACGT                
MroI         1535  TCCGGA               T^CCGGA
             2036  TCCGGA              
PflMI        1157  CCATGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2109  CCAGACGCTGG         
RsrII        1537  CGGACCG              CG^G[AT]CCG
             2270  CGGTCCG             
SacI         1261  GAGCTC               GAGCT^C
             2286  GAGCTC              
SacII           5  CCGCGG               CCGC^GG
               77  CCGCGG              
SexAI        1313  ACCTGGT              A^CC[AT]GGT
             2078  ACCAGGT             
SmlI         1168  CTTGAG               C^T[CT][GA]AG
             1332  CTCAAG              
StuI          428  AGGCCT               AGG^CCT
             2798  AGGCCT              
Van91I       1157  CCATGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2109  CCAGACGCTGG         
XcmI          501  CCAGTTCTCCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3001  CCACTGTTTCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EagI        EarI        EclXI       
Eco52I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI