##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BanI        BbeI        
BciVI       BclI        BfaI        BfrI        BlnI        
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BspDI       BspEI       BspLU11I    BsrBI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MunI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        Tth111I     Van91I      
XbaI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcyI         1211  GACGCC               G[GA]^CG[CT]C
Aor51HI       518  AGCGCT               AGC^GCT
AspHI         595  GAGCTC               G[AT]GC[AT]^C
BalI         1175  TGGCCA               TGG^CCA
BamHI        1922  GGATCC               G^GATCC
BbrPI        1192  CACGTG               CAC^GTG
BbsI          864  GAAGAC               GAAGAC(2/6)
BglII         552  AGATCT               A^GATCT
BpuAI         864  GAAGAC               GAAGAC(2/6)
BsaHI        1211  GACGCC               G[GA]^CG[CT]C
BsiEI         372  CGGCCG               CG[GA][CT]^CG
BsiHKAI       595  GAGCTC               G[AT]GC[AT]^C
BsmI          507  GAATGC               GAATGC(1/-1)
Bsp1407I     1863  TGTACA               T^GTACA
BspHI        1539  TCATGA               T^CATGA
BsrDI        1268  GCAATG               GCAATG(2/0)
BsrGI        1863  TGTACA               T^GTACA
BstAPI        115  GCAGAGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI          372  CGGCCG               C^GGCCG
EclXI         372  CGGCCG               C^GGCCG
Eco47III      518  AGCGCT               AGC^GCT
Eco52I        372  CGGCCG               C^GGCCG
Hin1I        1211  GACGCC               G[GA]^CG[CT]C
MluNI        1175  TGGCCA               TGG^CCA
MscI         1175  TGGCCA               TGG^CCA
MslI         1730  CATCAAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI         2069  ACATGC               [GA]CATG^[CT]
PaeR7I       1373  CTCGAG               C^TCGAG
PmaCI        1192  CACGTG               CAC^GTG
PmlI         1192  CACGTG               CAC^GTG
RcaI         1539  TCATGA               T^CATGA
SacI          595  GAGCTC               GAGCT^C
SfiI         1167  GGCCGACCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI        1863  TGTACA               T^GTACA
SspI         1930  AATATT               AAT^ATT
XhoI         1373  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII         235  CGCG                 CG^CG
             1230  CGCG                
ApaI          613  GGGCCC               GGGCC^C
             1090  GGGCCC              
AvaI          439  CCCGAG               C^[CT]CG[GA]G
             1373  CTCGAG              
BlpI           99  GCTGAGC              GC^T[ACGT]AGC
             1464  GCTCAGC             
Bpu1102I       99  GCTGAGC              GC^T[ACGT]AGC
             1464  GCTCAGC             
BsmBI         399  CGTCTC               CGTCTC(1/5)
             1491  GAGACG              
BsoBI         439  CCCGAG               C^[CT]CG[GA]G
             1373  CTCGAG              
BssSI         592  CACGAG               CACGAG(-5/-1)
             1936  CTCGTG              
BstUI         235  CGCG                 CG^CG
             1230  CGCG                
CelII          99  GCTGAGC              GC^T[ACGT]AGC
             1464  GCTCAGC             
HaeII         179  AGCGCC               [GA]GCGC^[CT]
              518  AGCGCT              
MvnI          235  CGCG                 CG^CG
             1230  CGCG                
NaeI          370  GCCGGC               GCC^GGC
              374  GCCGGC              
NgoMIV        370  GCCGGC               G^CCGGC
              374  GCCGGC              
PleI         1332  GAGTC                GAGTC(4/5)
             1376  GAGTC               
PpuMI         389  GGGTCCT              [GA]G^G[AT]CC[CT]
              644  AGGACCT             
TaqI           88  TCGA                 T^CGA
             1374  TCGA                
TthHB8I        88  TCGA                 T^CGA
             1374  TCGA                
XcmI          212  CCAGATCCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1163  CCAAGGCCGACCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BanII       BbvCI       BbvI        BcgI        
BcnI        BglI        BmyI        BpmI        BsaAI       
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII