################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BciVI BclI BfaI BfrI BlnI BsaBI BsaI BsaWI BseAI BsiWI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcyI 1211 GACGCC G[GA]^CG[CT]C Aor51HI 518 AGCGCT AGC^GCT AspHI 595 GAGCTC G[AT]GC[AT]^C BalI 1175 TGGCCA TGG^CCA BamHI 1922 GGATCC G^GATCC BbrPI 1192 CACGTG CAC^GTG BbsI 864 GAAGAC GAAGAC(2/6) BglII 552 AGATCT A^GATCT BpuAI 864 GAAGAC GAAGAC(2/6) BsaHI 1211 GACGCC G[GA]^CG[CT]C BsiEI 372 CGGCCG CG[GA][CT]^CG BsiHKAI 595 GAGCTC G[AT]GC[AT]^C BsmI 507 GAATGC GAATGC(1/-1) Bsp1407I 1863 TGTACA T^GTACA BspHI 1539 TCATGA T^CATGA BsrDI 1268 GCAATG GCAATG(2/0) BsrGI 1863 TGTACA T^GTACA BstAPI 115 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EagI 372 CGGCCG C^GGCCG EclXI 372 CGGCCG C^GGCCG Eco47III 518 AGCGCT AGC^GCT Eco52I 372 CGGCCG C^GGCCG Hin1I 1211 GACGCC G[GA]^CG[CT]C MluNI 1175 TGGCCA TGG^CCA MscI 1175 TGGCCA TGG^CCA MslI 1730 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 2069 ACATGC [GA]CATG^[CT] PaeR7I 1373 CTCGAG C^TCGAG PmaCI 1192 CACGTG CAC^GTG PmlI 1192 CACGTG CAC^GTG RcaI 1539 TCATGA T^CATGA SacI 595 GAGCTC GAGCT^C SfiI 1167 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 1863 TGTACA T^GTACA SspI 1930 AATATT AAT^ATT XhoI 1373 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 235 CGCG CG^CG 1230 CGCG ApaI 613 GGGCCC GGGCC^C 1090 GGGCCC AvaI 439 CCCGAG C^[CT]CG[GA]G 1373 CTCGAG BlpI 99 GCTGAGC GC^T[ACGT]AGC 1464 GCTCAGC Bpu1102I 99 GCTGAGC GC^T[ACGT]AGC 1464 GCTCAGC BsmBI 399 CGTCTC CGTCTC(1/5) 1491 GAGACG BsoBI 439 CCCGAG C^[CT]CG[GA]G 1373 CTCGAG BssSI 592 CACGAG CACGAG(-5/-1) 1936 CTCGTG BstUI 235 CGCG CG^CG 1230 CGCG CelII 99 GCTGAGC GC^T[ACGT]AGC 1464 GCTCAGC HaeII 179 AGCGCC [GA]GCGC^[CT] 518 AGCGCT MvnI 235 CGCG CG^CG 1230 CGCG NaeI 370 GCCGGC GCC^GGC 374 GCCGGC NgoMIV 370 GCCGGC G^CCGGC 374 GCCGGC PleI 1332 GAGTC GAGTC(4/5) 1376 GAGTC PpuMI 389 GGGTCCT [GA]G^G[AT]CC[CT] 644 AGGACCT TaqI 88 TCGA T^CGA 1374 TCGA TthHB8I 88 TCGA T^CGA 1374 TCGA XcmI 212 CCAGATCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1163 CCAAGGCCGACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanII BbvCI BbvI BcgI BcnI BglI BmyI BpmI BsaAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII