##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AccIII      AclI        AgeI        AscI        AvrII       
BclI        BglII       BlnI        BseAI       BsiWI       
BspDI       BspEI       BssHII      Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
EagI        EclXI       Eco52I      EcoRI       EcoRV       
FbaI        FseI        HpaI        MfeI        MroI        
MunI        NheI        NotI        NruI        NspV        
PacI        PinAI       PmeI        Psp1406I    RsrII       
SalI        ScaI        SfiI        SfuI        SmaI        
SnaBI       SpeI        SspI        SwaI        XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        5806  GACGTC               GACGT^C
Acc65I       2375  GGTACC               G^GTACC
AccI         2903  GTCTAC               GT^[AC][GT]AC
AhdI         1730  GACTGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4886  GTGCAC               G^TGCAC
ApaLI        4886  GTGCAC               G^TGCAC
AseI         2358  ATTAAT               AT^TAAT
AsnI         2358  ATTAAT               AT^TAAT
Asp700I      1044  GAACTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2375  GGTACC               G^GTACC
AspEI        1730  GACTGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          888  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1087  GGATCC               G^GATCC
BbeI         4060  GGCGCC               GGCGC^C
BlpI         5925  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     5925  GCTGAGC              GC^T[ACGT]AGC
BsaBI        6015  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI        6288  CCGCTC               CCGCTC(-3/-3)
BsrDI        2457  CATTGC               GCAATG(2/0)
CelII        5925  GCTGAGC              GC^T[ACGT]AGC
DraIII       6095  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1730  GACTGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII       6067  GTTGAC               GT[CT]^[GA]AC
HindII       6067  GTTGAC               GT[CT]^[GA]AC
HindIII      2183  AAGCTT               A^AGCTT
KasI         4060  GGCGCC               G^GCGCC
KpnI         2375  GGTACC               GGTAC^C
MamI         6015  GATCAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         3514  ACGCGT               A^CGCGT
NarI         4060  GGCGCC               GG^CGCC
NdeI         2946  CATATG               CA^TATG
PaeR7I       1039  CTCGAG               C^TCGAG
PflFI         888  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        5043  CCAGCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        2358  ATTAAT               AT^TAAT
SacI         6487  GAGCTC               GAGCT^C
SapI          969  GAAGAGC              GCTCTTC(1/4)
SbfI         2111  CCTGCAGG             CCTGCA^GG
SexAI        3387  ACCAGGT              A^CC[AT]GGT
SfoI         4060  GGCGCC               GGC^GCC
SgrAI        6038  CACCGGCG             C[GA]^CCGG[CT]G
Sse8387I     2111  CCTGCAGG             CCTGCA^GG
Tth111I       888  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       5043  CCAGCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1039  CTCGAG               C^TCGAG
XmnI         1044  GAACTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AflII        1898  CTTAAG               C^TTAAG
             3295  CTTAAG              
Aor51HI      3964  AGCGCT               AGC^GCT
             6432  AGCGCT              
ApaI         3365  GGGCCC               GGGCC^C
             5395  GGGCCC              
AviII        4893  TGCGCA               TGC^GCA
             5016  TGCGCA              
BcgI         2775  CGAGACCCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             6699  GCAGGAACCTCG        
BciVI          74  GTATCC               GTATCC(6/5)
             3947  GTATCC              
BfrI         1898  CTTAAG               C^TTAAG
             3295  CTTAAG              
BglI         2689  GCCTTACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3776  GCCTGGCAGGC         
BsaWI        1084  ACCGGA               [AT]^CCGG[AT]
             6669  ACCGGA              
BsmBI        3004  GAGACG               CGTCTC(1/5)
             5530  GAGACG              
BspLU11I     4387  ACATGT               A^CATGT
             6335  ACATGT              
BstAPI       3988  GCAGGGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             5225  GCAGAGGCTGC         
Eco47III     3964  AGCGCT               AGC^GCT
             6432  AGCGCT              
EcoT22I       156  ATGCAT               ATGCA^T
             3873  ATGCAT              
FspI         4893  TGCGCA               TGC^GCA
             5016  TGCGCA              
KspI         6152  CCGCGG               CCGC^GG
             6570  CCGCGG              
NsiI          156  ATGCAT               ATGCA^T
             3873  ATGCAT              
SacII        6152  CCGCGG               CCGC^GG
             6570  CCGCGG              


#########################################
# 3 OR MORE CUT ENZYMES (  142 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApoI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbrPI       BbsI        BbvCI       
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspHI       BspMI       BsrFI       
BsrGI       BsrI        BssKI       BssSI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PleI        PmaCI       PmlI        
PpuMI       PshAI       PstI        PvuI        PvuII       
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SphI        SspBI       
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII