##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AccIII AclI AgeI AscI AvrII
BclI BglII BlnI BseAI BsiWI
BspDI BspEI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoRI EcoRV
FbaI FseI HpaI MfeI MroI
MunI NheI NotI NruI NspV
PacI PinAI PmeI Psp1406I RsrII
SalI ScaI SfiI SfuI SmaI
SnaBI SpeI SspI SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 5806 GACGTC GACGT^C
Acc65I 2375 GGTACC G^GTACC
AccI 2903 GTCTAC GT^[AC][GT]AC
AhdI 1730 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4886 GTGCAC G^TGCAC
ApaLI 4886 GTGCAC G^TGCAC
AseI 2358 ATTAAT AT^TAAT
AsnI 2358 ATTAAT AT^TAAT
Asp700I 1044 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2375 GGTACC G^GTACC
AspEI 1730 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 888 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1087 GGATCC G^GATCC
BbeI 4060 GGCGCC GGCGC^C
BlpI 5925 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5925 GCTGAGC GC^T[ACGT]AGC
BsaBI 6015 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI 6288 CCGCTC CCGCTC(-3/-3)
BsrDI 2457 CATTGC GCAATG(2/0)
CelII 5925 GCTGAGC GC^T[ACGT]AGC
DraIII 6095 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1730 GACTGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 6067 GTTGAC GT[CT]^[GA]AC
HindII 6067 GTTGAC GT[CT]^[GA]AC
HindIII 2183 AAGCTT A^AGCTT
KasI 4060 GGCGCC G^GCGCC
KpnI 2375 GGTACC GGTAC^C
MamI 6015 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3514 ACGCGT A^CGCGT
NarI 4060 GGCGCC GG^CGCC
NdeI 2946 CATATG CA^TATG
PaeR7I 1039 CTCGAG C^TCGAG
PflFI 888 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 5043 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2358 ATTAAT AT^TAAT
SacI 6487 GAGCTC GAGCT^C
SapI 969 GAAGAGC GCTCTTC(1/4)
SbfI 2111 CCTGCAGG CCTGCA^GG
SexAI 3387 ACCAGGT A^CC[AT]GGT
SfoI 4060 GGCGCC GGC^GCC
SgrAI 6038 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 2111 CCTGCAGG CCTGCA^GG
Tth111I 888 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 5043 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1039 CTCGAG C^TCGAG
XmnI 1044 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflII 1898 CTTAAG C^TTAAG
3295 CTTAAG
Aor51HI 3964 AGCGCT AGC^GCT
6432 AGCGCT
ApaI 3365 GGGCCC GGGCC^C
5395 GGGCCC
AviII 4893 TGCGCA TGC^GCA
5016 TGCGCA
BcgI 2775 CGAGACCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6699 GCAGGAACCTCG
BciVI 74 GTATCC GTATCC(6/5)
3947 GTATCC
BfrI 1898 CTTAAG C^TTAAG
3295 CTTAAG
BglI 2689 GCCTTACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3776 GCCTGGCAGGC
BsaWI 1084 ACCGGA [AT]^CCGG[AT]
6669 ACCGGA
BsmBI 3004 GAGACG CGTCTC(1/5)
5530 GAGACG
BspLU11I 4387 ACATGT A^CATGT
6335 ACATGT
BstAPI 3988 GCAGGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5225 GCAGAGGCTGC
Eco47III 3964 AGCGCT AGC^GCT
6432 AGCGCT
EcoT22I 156 ATGCAT ATGCA^T
3873 ATGCAT
FspI 4893 TGCGCA TGC^GCA
5016 TGCGCA
KspI 6152 CCGCGG CCGC^GG
6570 CCGCGG
NsiI 156 ATGCAT ATGCA^T
3873 ATGCAT
SacII 6152 CCGCGG CCGC^GG
6570 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspMI BsrFI
BsrGI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PshAI PstI PvuI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII