################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII AccI AclI AcyI AgeI AhdI Aor51HI ApaI AscI AspEI AspI AviII AvrII BbeI BcgI BcnI BlnI BlpI Bpu1102I BsaHI BsiEI BsiWI BsmBI BspDI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstZ17I CelII CfoI Cfr10I ClaI CpoI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I FauI FseI FspI HaeII HhaI Hin1I HinP1I KasI KspI MluI NaeI NarI NciI NcoI NgoMIV NheI NotI PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### Acc65I 89 GGTACC G^GTACC AccII 48 CGCG CG^CG AccIII 71 TCCGGA T^CCGGA AflII 235 CTTAAG C^TTAAG Alw44I 4297 GTGCAC G^TGCAC AlwNI 3958 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 905 CCTTAGG CC^T[ACGT]AGG ApaLI 4297 GTGCAC G^TGCAC Asp700I 1199 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 89 GGTACC G^GTACC AvaI 1644 CTCGAG C^[CT]CG[GA]G BalI 658 TGGCCA TGG^CCA BanI 89 GGTACC G^G[CT][GA]CC BanII 3710 GAGCTC G[GA]GC[CT]^C BbrPI 1603 CACGTG CAC^GTG BbvCI 2212 GCTGAGG CCTCAGC(-5/-2) BfrI 235 CTTAAG C^TTAAG BpmI 2396 CTCCAG CTGGAG(16/14) BsaI 526 GAGACC GGTCTC(1/5) BsaWI 71 TCCGGA [AT]^CCGG[AT] BseAI 71 TCCGGA T^CCGGA BsmI 3348 GCATTC GAATGC(1/-1) BsoBI 1644 CTCGAG C^[CT]CG[GA]G BspEI 71 TCCGGA T^CCGGA BspLU11I 3553 ACATGT A^CATGT BsrBI 14 GAGCGG CCGCTC(-3/-3) BstAPI 1435 GCATTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3354 TTCGAA TT^CGAA BstUI 48 CGCG CG^CG BstXI 1656 CCACCATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 905 CCTTAGG CC^T[ACGT]AGG Cac8I 2361 GCAAGC GC[ACGT]^[ACGT]GC DrdI 2638 GACAGTAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 658 TGGCCA [CT]^GGCC[GA] EarI 2695 CTCTTC CTCTTC(1/4) Eco81I 905 CCTTAGG CC^T[ACGT]AGG HapII 72 CCGG C^CGG HgaI 51 GACGC GACGC(5/10) HindIII 3780 AAGCTT A^AGCTT HpaI 3855 GTTAAC GTT^AAC HpaII 72 CCGG C^CGG KpnI 89 GGTACC GGTAC^C Ksp632I 2695 CTCTTC CTCTTC(1/4) MluNI 658 TGGCCA TGG^CCA MroI 71 TCCGGA T^CCGGA MscI 658 TGGCCA TGG^CCA MspI 72 CCGG C^CGG MvnI 48 CGCG CG^CG NdeI 2687 CATATG CA^TATG NruI 47 TCGCGA TCG^CGA NspV 3354 TTCGAA TT^CGAA PacI 2506 TTAATTAA TTAAT^TAA PaeR7I 1644 CTCGAG C^TCGAG PleI 3954 GAGTC GAGTC(4/5) PmaCI 1603 CACGTG CAC^GTG PmlI 1603 CACGTG CAC^GTG SacI 3710 GAGCTC GAGCT^C SexAI 85 ACCTGGT A^CC[AT]GGT SfiI 772 GGCCTCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 3354 TTCGAA TT^CGAA XbaI 3515 TCTAGA T^CTAGA XhoI 1644 CTCGAG C^TCGAG XmnI 1199 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AflIII 802 ACGTGT A^C[GA][CT]GT 3553 ACATGT BclI 947 TGATCA T^GATCA 4381 TGATCA BglI 773 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1841 GCCCAAATGGC BglII 1422 AGATCT A^GATCT 2149 AGATCT BsaAI 801 TACGTG [CT]AC^GT[GA] 1603 CACGTG BsaBI 871 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2167 GATTGGGATC BseRI 1967 GAGGAG GAGGAG(10/8) 2576 GAGGAG BsgI 4522 CTGCAC GTGCAG(16/14) 3004 GTGCAG Bsp1407I 1429 TGTACA T^GTACA 3965 TGTACA BspHI 809 TCATGA T^CATGA 3415 TCATGA BspMI 1458 ACCTGC ACCTGC(4/8) 2521 GCAGGT BsrDI 1313 CATTGC GCAATG(2/0) 2807 GCAATG BsrGI 1429 TGTACA T^GTACA 3965 TGTACA DraII 666 GGGTCCT [GA]G^G[ACGT]CC[CT] 2523 AGGTCCT DraIII 1868 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG 4287 CACTCTGTG EcoO109I 666 GGGTCCT [GA]G^G[ACGT]CC[CT] 2523 AGGTCCT EcoRI 2500 GAATTC G^AATTC 3882 GAATTC EcoRV 1037 GATATC GAT^ATC 2080 GATATC EcoT22I 3622 ATGCAT ATGCA^T 4351 ATGCAT FbaI 947 TGATCA T^GATCA 4381 TGATCA HincII 2018 GTCAAC GT[CT]^[GA]AC 3855 GTTAAC HindII 2018 GTCAAC GT[CT]^[GA]AC 3855 GTTAAC MamI 871 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2167 GATTGGGATC MfeI 479 CAATTG C^AATTG 3832 CAATTG MunI 479 CAATTG C^AATTG 3832 CAATTG NsiI 3622 ATGCAT ATGCA^T 4351 ATGCAT PflMI 530 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3685 CCATTTTGTGG PpuMI 666 GGGTCCT [GA]G^G[AT]CC[CT] 2523 AGGTCCT PstI 1460 CTGCAG CTGCA^G 3315 CTGCAG PvuII 303 CAGCTG CAG^CTG 2768 CAGCTG RcaI 809 TCATGA T^CATGA 3415 TCATGA ScaI 2400 AGTACT AGT^ACT 3008 AGTACT SfcI 1460 CTGCAG C^T[GA][CT]AG 3315 CTGCAG SspBI 1429 TGTACA T^GTACA 3965 TGTACA SspI 1509 AATATT AAT^ATT 1825 AATATT StuI 771 AGGCCT AGG^CCT 4596 AGGCCT Tsp45I 231 GTCAC ^GT[GC]AC 982 GTCAC Van91I 530 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3685 CCATTTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AseI AsnI AspHI AvaII BamHI BbsI BbvI BciVI BfaI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XcmI XhoII