##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       AccI        AclI        AcyI        AgeI        
AhdI        Aor51HI     ApaI        AscI        AspEI       
AspI        AviII       AvrII       BbeI        BcgI        
BcnI        BlnI        BlpI        Bpu1102I    BsaHI       
BsiEI       BsiWI       BsmBI       BspDI       BsrFI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     FauI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      KasI        KspI        MluI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        PflFI       PinAI       PmeI        PshAI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        SbfI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SwaI        
Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

Acc65I         89  GGTACC               G^GTACC
AccII          48  CGCG                 CG^CG
AccIII         71  TCCGGA               T^CCGGA
AflII         235  CTTAAG               C^TTAAG
Alw44I       4297  GTGCAC               G^TGCAC
AlwNI        3958  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          905  CCTTAGG              CC^T[ACGT]AGG
ApaLI        4297  GTGCAC               G^TGCAC
Asp700I      1199  GAAATGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I        89  GGTACC               G^GTACC
AvaI         1644  CTCGAG               C^[CT]CG[GA]G
BalI          658  TGGCCA               TGG^CCA
BanI           89  GGTACC               G^G[CT][GA]CC
BanII        3710  GAGCTC               G[GA]GC[CT]^C
BbrPI        1603  CACGTG               CAC^GTG
BbvCI        2212  GCTGAGG              CCTCAGC(-5/-2)
BfrI          235  CTTAAG               C^TTAAG
BpmI         2396  CTCCAG               CTGGAG(16/14)
BsaI          526  GAGACC               GGTCTC(1/5)
BsaWI          71  TCCGGA               [AT]^CCGG[AT]
BseAI          71  TCCGGA               T^CCGGA
BsmI         3348  GCATTC               GAATGC(1/-1)
BsoBI        1644  CTCGAG               C^[CT]CG[GA]G
BspEI          71  TCCGGA               T^CCGGA
BspLU11I     3553  ACATGT               A^CATGT
BsrBI          14  GAGCGG               CCGCTC(-3/-3)
BstAPI       1435  GCATTGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        3354  TTCGAA               TT^CGAA
BstUI          48  CGCG                 CG^CG
BstXI        1656  CCACCATACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        905  CCTTAGG              CC^T[ACGT]AGG
Cac8I        2361  GCAAGC               GC[ACGT]^[ACGT]GC
DrdI         2638  GACAGTAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          658  TGGCCA               [CT]^GGCC[GA]
EarI         2695  CTCTTC               CTCTTC(1/4)
Eco81I        905  CCTTAGG              CC^T[ACGT]AGG
HapII          72  CCGG                 C^CGG
HgaI           51  GACGC                GACGC(5/10)
HindIII      3780  AAGCTT               A^AGCTT
HpaI         3855  GTTAAC               GTT^AAC
HpaII          72  CCGG                 C^CGG
KpnI           89  GGTACC               GGTAC^C
Ksp632I      2695  CTCTTC               CTCTTC(1/4)
MluNI         658  TGGCCA               TGG^CCA
MroI           71  TCCGGA               T^CCGGA
MscI          658  TGGCCA               TGG^CCA
MspI           72  CCGG                 C^CGG
MvnI           48  CGCG                 CG^CG
NdeI         2687  CATATG               CA^TATG
NruI           47  TCGCGA               TCG^CGA
NspV         3354  TTCGAA               TT^CGAA
PacI         2506  TTAATTAA             TTAAT^TAA
PaeR7I       1644  CTCGAG               C^TCGAG
PleI         3954  GAGTC                GAGTC(4/5)
PmaCI        1603  CACGTG               CAC^GTG
PmlI         1603  CACGTG               CAC^GTG
SacI         3710  GAGCTC               GAGCT^C
SexAI          85  ACCTGGT              A^CC[AT]GGT
SfiI          772  GGCCTCTGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         3354  TTCGAA               TT^CGAA
XbaI         3515  TCTAGA               T^CTAGA
XhoI         1644  CTCGAG               C^TCGAG
XmnI         1199  GAAATGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AflIII        802  ACGTGT               A^C[GA][CT]GT
             3553  ACATGT              
BclI          947  TGATCA               T^GATCA
             4381  TGATCA              
BglI          773  GCCTCTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1841  GCCCAAATGGC         
BglII        1422  AGATCT               A^GATCT
             2149  AGATCT              
BsaAI         801  TACGTG               [CT]AC^GT[GA]
             1603  CACGTG              
BsaBI         871  GATAAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2167  GATTGGGATC          
BseRI        1967  GAGGAG               GAGGAG(10/8)
             2576  GAGGAG              
BsgI         4522  CTGCAC               GTGCAG(16/14)
             3004  GTGCAG              
Bsp1407I     1429  TGTACA               T^GTACA
             3965  TGTACA              
BspHI         809  TCATGA               T^CATGA
             3415  TCATGA              
BspMI        1458  ACCTGC               ACCTGC(4/8)
             2521  GCAGGT              
BsrDI        1313  CATTGC               GCAATG(2/0)
             2807  GCAATG              
BsrGI        1429  TGTACA               T^GTACA
             3965  TGTACA              
DraII         666  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             2523  AGGTCCT             
DraIII       1868  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4287  CACTCTGTG           
EcoO109I      666  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             2523  AGGTCCT             
EcoRI        2500  GAATTC               G^AATTC
             3882  GAATTC              
EcoRV        1037  GATATC               GAT^ATC
             2080  GATATC              
EcoT22I      3622  ATGCAT               ATGCA^T
             4351  ATGCAT              
FbaI          947  TGATCA               T^GATCA
             4381  TGATCA              
HincII       2018  GTCAAC               GT[CT]^[GA]AC
             3855  GTTAAC              
HindII       2018  GTCAAC               GT[CT]^[GA]AC
             3855  GTTAAC              
MamI          871  GATAAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2167  GATTGGGATC          
MfeI          479  CAATTG               C^AATTG
             3832  CAATTG              
MunI          479  CAATTG               C^AATTG
             3832  CAATTG              
NsiI         3622  ATGCAT               ATGCA^T
             4351  ATGCAT              
PflMI         530  CCAGATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3685  CCATTTTGTGG         
PpuMI         666  GGGTCCT              [GA]G^G[AT]CC[CT]
             2523  AGGTCCT             
PstI         1460  CTGCAG               CTGCA^G
             3315  CTGCAG              
PvuII         303  CAGCTG               CAG^CTG
             2768  CAGCTG              
RcaI          809  TCATGA               T^CATGA
             3415  TCATGA              
ScaI         2400  AGTACT               AGT^ACT
             3008  AGTACT              
SfcI         1460  CTGCAG               C^T[GA][CT]AG
             3315  CTGCAG              
SspBI        1429  TGTACA               T^GTACA
             3965  TGTACA              
SspI         1509  AATATT               AAT^ATT
             1825  AATATT              
StuI          771  AGGCCT               AGG^CCT
             4596  AGGCCT              
Tsp45I        231  GTCAC                ^GT[GC]AC
              982  GTCAC               
Van91I        530  CCAGATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3685  CCATTTTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AseI        AsnI        AspHI       AvaII       
BamHI       BbsI        BbvI        BciVI       BfaI        
BmyI        BpuAI       BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cfr13I      
DdeI        DpnII       DraI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PshBI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XcmI        XhoII