##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AccIII AclI AgeI Alw44I ApaI
ApaLI AscI AvrII BbvCI BciVI
BglI BlnI BsaI BseAI BsiWI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII BstBI ClaI CpoI EcoRV
FseI KspI MluI MroI NheI
NotI NruI NspV PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1143 GACGTC GACGT^C
Acc65I 164 GGTACC G^GTACC
AflII 2492 CTTAAG C^TTAAG
AhdI 402 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 164 GGTACC G^GTACC
AspEI 402 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4779 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 65 TGCGCA TGC^GCA
BamHI 2798 GGATCC G^GATCC
BbrPI 4673 CACGTG CAC^GTG
BfrI 2492 CTTAAG C^TTAAG
BsaAI 4673 CACGTG [CT]AC^GT[GA]
BsaBI 2528 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 241 CGTCTC CGTCTC(1/5)
BsmI 4570 GAATGC GAATGC(1/-1)
BspHI 6068 TCATGA T^CATGA
BssSI 1033 CACGAG CACGAG(-5/-1)
Bst1107I 3675 GTATAC GTA^TAC
BstAPI 2169 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 5590 GGTAACC G^GT[ACGT]ACC
BstPI 5590 GGTAACC G^GT[ACGT]ACC
BstZ17I 3675 GTATAC GTA^TAC
DsaI 2395 CCATGG C^C[GA][CT]GG
Eam1105I 402 GACTTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5590 GGTAACC G^GT[ACGT]ACC
FspI 65 TGCGCA TGC^GCA
HgaI 240 GCGTC GACGC(5/10)
HpaI 888 GTTAAC GTT^AAC
KpnI 164 GGTACC GGTAC^C
MamI 2528 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2410 CAATTG C^AATTG
MunI 2410 CAATTG C^AATTG
NcoI 2395 CCATGG C^CATGG
PacI 3798 TTAATTAA TTAAT^TAA
PaeR7I 389 CTCGAG C^TCGAG
PflFI 4779 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4673 CACGTG CAC^GTG
PmlI 4673 CACGTG CAC^GTG
RcaI 6068 TCATGA T^CATGA
ScaI 5215 AGTACT AGT^ACT
SexAI 1108 ACCTGGT A^CC[AT]GGT
Tth111I 4779 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 389 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AflIII 4046 ACATGT A^C[GA][CT]GT
5500 ACATGT
AlwNI 1125 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
4966 CAGAGACTG
AocI 2006 CCTGAGG CC^T[ACGT]AGG
5854 CCTGAGG
Aor51HI 49 AGCGCT AGC^GCT
256 AGCGCT
BalI 103 TGGCCA TGG^CCA
2041 TGGCCA
BbeI 15 GGCGCC GGCGC^C
1964 GGCGCC
BbsI 1055 GTCTTC GAAGAC(2/6)
6041 GTCTTC
BclI 3176 TGATCA T^GATCA
3926 TGATCA
BlpI 3187 GCTTAGC GC^T[ACGT]AGC
3192 GCTTAGC
Bpu1102I 3187 GCTTAGC GC^T[ACGT]AGC
3192 GCTTAGC
BpuAI 1055 GTCTTC GAAGAC(2/6)
6041 GTCTTC
BsaWI 167 ACCGGA [AT]^CCGG[AT]
2231 TCCGGT
BsiEI 58 CGGCCG CG[GA][CT]^CG
128 CGGCCG
BspLU11I 4046 ACATGT A^CATGT
5500 ACATGT
BsrDI 1003 GCAATG GCAATG(2/0)
4442 GCAATG
Bsu36I 2006 CCTGAGG CC^T[ACGT]AGG
5854 CCTGAGG
CelII 3187 GCTTAGC GC^T[ACGT]AGC
3192 GCTTAGC
DraIII 4740 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
4908 CACAGTGTG
DrdI 1827 GACTCATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3057 GACCATCCTGTC
EagI 58 CGGCCG C^GGCCG
128 CGGCCG
EclXI 58 CGGCCG C^GGCCG
128 CGGCCG
Eco47III 49 AGCGCT AGC^GCT
256 AGCGCT
Eco52I 58 CGGCCG C^GGCCG
128 CGGCCG
Eco81I 2006 CCTGAGG CC^T[ACGT]AGG
5854 CCTGAGG
EcoRI 3474 GAATTC G^AATTC
3789 GAATTC
FbaI 3176 TGATCA T^GATCA
3926 TGATCA
KasI 15 GGCGCC G^GCGCC
1964 GGCGCC
MluNI 103 TGGCCA TGG^CCA
2041 TGGCCA
MscI 103 TGGCCA TGG^CCA
2041 TGGCCA
NaeI 26 GCCGGC GCC^GGC
1961 GCCGGC
NarI 15 GGCGCC GG^CGCC
1964 GGCGCC
NdeI 1831 CATATG CA^TATG
2248 CATATG
NgoMIV 26 GCCGGC G^CCGGC
1961 GCCGGC
PpuMI 1282 AGGTCCC [GA]G^G[AT]CC[CT]
2026 AGGACCT
SacI 1702 GAGCTC GAGCT^C
2112 GAGCTC
SalI 502 GTCGAC G^TCGAC
5631 GTCGAC
SapI 484 GCTCTTC GCTCTTC(1/4)
1798 GCTCTTC
SfoI 15 GGCGCC GGC^GCC
1964 GGCGCC
SmaI 31 CCCGGG CCC^GGG
224 CCCGGG
XbaI 2337 TCTAGA T^CTAGA
2861 TCTAGA
XcmI 3509 CCAAGTTTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5814 CCAGGTGGATTCTGG
XmaCI 31 CCCGGG C^CCGGG
224 CCCGGG
XmaI 31 CCCGGG C^CCGGG
224 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BanI BanII BbvI BcgI BcnI
BfaI BglII BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI