##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AgeI AhdI AscI AseI
AsnI AspEI AviII AvrII BamHI
BbeI BbrPI BcgI BlnI BsaAI
BsiWI Bsp1407I BsrGI Bst1107I BstZ17I
CpoI EagI Eam1105I EclXI Eco52I
FseI FspI HpaI KasI KspI
MluI NarI NdeI NotI NruI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI RsrII SacII ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI SspBI SspI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 70 GGTACC G^GTACC
AccII 980 CGCG CG^CG
AccIII 3123 TCCGGA T^CCGGA
AclI 5170 AACGTT AA^CGTT
AflII 1115 CTTAAG C^TTAAG
Alw44I 43 GTGCAC G^TGCAC
Aor51HI 2823 AGCGCT AGC^GCT
ApaLI 43 GTGCAC G^TGCAC
Asp718I 70 GGTACC G^GTACC
AvaI 1366 CTCGGG C^[CT]CG[GA]G
BbvCI 4199 CCTCAGC CCTCAGC(-5/-2)
BfrI 1115 CTTAAG C^TTAAG
BglI 1895 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 3123 TCCGGA T^CCGGA
BsmBI 3410 CGTCTC CGTCTC(1/5)
BsmI 559 GAATGC GAATGC(1/-1)
BsoBI 1366 CTCGGG C^[CT]CG[GA]G
BspDI 143 ATCGAT AT^CGAT
BspEI 3123 TCCGGA T^CCGGA
BspLU11I 4902 ACATGT A^CATGT
BspMI 190 ACCTGC ACCTGC(4/8)
BsrBI 3293 GAGCGG CCGCTC(-3/-3)
BssHII 979 GCGCGC G^CGCGC
BstBI 2563 TTCGAA TT^CGAA
BstUI 980 CGCG CG^CG
BstXI 4881 CCACGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 143 ATCGAT AT^CGAT
DraIII 6476 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2823 AGCGCT AGC^GCT
EcoRV 1997 GATATC GAT^ATC
EcoT22I 5377 ATGCAT ATGCA^T
KpnI 70 GGTACC GGTAC^C
MroI 3123 TCCGGA T^CCGGA
MvnI 980 CGCG CG^CG
NaeI 3171 GCCGGC GCC^GGC
NgoMIV 3171 GCCGGC G^CCGGC
NsiI 5377 ATGCAT ATGCA^T
NspV 2563 TTCGAA TT^CGAA
PacI 6543 TTAATTAA TTAAT^TAA
PpuMI 4431 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 891 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5170 AACGTT AA^CGTT
PvuI 1830 CGATCG CGAT^CG
SbfI 1723 CCTGCAGG CCTGCA^GG
SfuI 2563 TTCGAA TT^CGAA
SpeI 5820 ACTAGT A^CTAGT
Sse8387I 1723 CCTGCAGG CCTGCA^GG
StuI 723 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 4902 ACATGT A^C[GA][CT]GT
6419 ACGTGT
AocI 1306 CCTGAGG CC^T[ACGT]AGG
5685 CCTGAGG
ApaI 2811 GGGCCC GGGCC^C
4106 GGGCCC
AspI 2057 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3936 GACATGGTC
BalI 161 TGGCCA TGG^CCA
6504 TGGCCA
BciVI 2717 GGATAC GTATCC(6/5)
3030 GTATCC
BclI 759 TGATCA T^GATCA
3510 TGATCA
BglII 2038 AGATCT A^GATCT
3367 AGATCT
BsaBI 2959 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3025 GATTTGTATC
BsiEI 1830 CGATCG CG[GA][CT]^CG
2061 CGGTCG
BsrFI 393 GCCGGT [GA]^CCGG[CT]
3171 GCCGGC
Bsu36I 1306 CCTGAGG CC^T[ACGT]AGG
5685 CCTGAGG
Cfr10I 393 GCCGGT [GA]^CCGG[CT]
3171 GCCGGC
DraII 2810 AGGGCCC [GA]G^G[ACGT]CC[CT]
4431 AGGACCC
Eco81I 1306 CCTGAGG CC^T[ACGT]AGG
5685 CCTGAGG
EcoNI 1181 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6074 CCTCCTGAAGG
EcoO109I 2810 AGGGCCC [GA]G^G[ACGT]CC[CT]
4431 AGGACCC
FbaI 759 TGATCA T^GATCA
3510 TGATCA
HaeII 2823 AGCGCT [GA]GCGC^[CT]
4257 AGCGCC
MamI 2959 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3025 GATTTGTATC
MluNI 161 TGGCCA TGG^CCA
6504 TGGCCA
MscI 161 TGGCCA TGG^CCA
6504 TGGCCA
NcoI 3828 CCATGG C^CATGG
6033 CCATGG
NheI 4998 GCTAGC G^CTAGC
6246 GCTAGC
PflFI 2057 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3936 GACATGGTC
SalI 1351 GTCGAC G^TCGAC
4157 GTCGAC
SapI 5 GAAGAGC GCTCTTC(1/4)
3682 GAAGAGC
Tth111I 2057 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3936 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BsrDI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DrdI DsaI
EaeI EarI EcoO65I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmnI