##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccII AcyI AgeI AhdI
ApaI AscI AspEI AviII BbeI
BbrPI BciVI BclI BglI BglII
BlpI Bpu1102I BsaAI BsaHI BsiEI
BsiWI BspHI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO109I EcoO65I FbaI
FseI FspI HgaI Hin1I KasI
KspI MfeI MluI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2140 GGTACC G^GTACC
AccI 2036 GTAGAC GT^[AC][GT]AC
AccIII 1804 TCCGGA T^CCGGA
AclI 4555 AACGTT AA^CGTT
Aor51HI 3251 AGCGCT AGC^GCT
Asp718I 2140 GGTACC G^GTACC
AspI 3686 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 351 GGACC G^G[AT]CC
AvrII 3636 CCTAGG C^CTAGG
BanII 1623 GGGCTC G[GA]GC[CT]^C
BcgI 3768 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3636 CCTAGG C^CTAGG
BsaWI 1804 TCCGGA [AT]^CCGG[AT]
BseAI 1804 TCCGGA T^CCGGA
BseRI 5309 CTCCTC GAGGAG(10/8)
Bsp1407I 691 TGTACA T^GTACA
BspDI 2454 ATCGAT AT^CGAT
BspEI 1804 TCCGGA T^CCGGA
BsrGI 691 TGTACA T^GTACA
BstXI 834 CCATCCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2454 ATCGAT AT^CGAT
Eco47III 3251 AGCGCT AGC^GCT
EcoRV 5856 GATATC GAT^ATC
FauI 1758 CCCGC CCCGC(4/6)
HaeII 3251 AGCGCT [GA]GCGC^[CT]
HpaI 4999 GTTAAC GTT^AAC
KpnI 2140 GGTACC GGTAC^C
MroI 1804 TCCGGA T^CCGGA
NcoI 1107 CCATGG C^CATGG
NdeI 3303 CATATG CA^TATG
PflFI 3686 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 4555 AACGTT AA^CGTT
SapI 1856 GAAGAGC GCTCTTC(1/4)
SmaI 3756 CCCGGG CCC^GGG
SpeI 2631 ACTAGT A^CTAGT
SspBI 691 TGTACA T^GTACA
SwaI 3569 ATTTAAAT ATTT^AAAT
Tth111I 3686 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4304 TCTAGA T^CTAGA
XmaCI 3756 CCCGGG C^CCGGG
XmaI 3756 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 4820 CTTAAG C^TTAAG
6243 CTTAAG
AseI 5280 ATTAAT AT^TAAT
6262 ATTAAT
AsnI 5280 ATTAAT AT^TAAT
6262 ATTAAT
Asp700I 1334 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4459 GAAACCATTC
BalI 2960 TGGCCA TGG^CCA
4282 TGGCCA
BamHI 1637 GGATCC G^GATCC
3358 GGATCC
BanI 2140 GGTACC G^G[CT][GA]CC
6429 GGCACC
BbvCI 1080 CCTCAGC CCTCAGC(-5/-2)
3779 CCTCAGC
BfrI 4820 CTTAAG C^TTAAG
6243 CTTAAG
BsgI 2553 GTGCAG GTGCAG(16/14)
5532 GTGCAG
BsmBI 1312 CGTCTC CGTCTC(1/5)
1575 GAGACG
BsmFI 256 GGGAC GGGAC(10/14)
3799 GGGAC
BsmI 1766 GAATGC GAATGC(1/-1)
2428 GCATTC
BstAPI 1761 GCAGTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3743 GCAACTTCTGC
CfoI 3252 GCGC GCG^C
3725 GCGC
DsaI 1107 CCATGG C^C[GA][CT]GG
1642 CCACGG
EaeI 2960 TGGCCA [CT]^GGCC[GA]
4282 TGGCCA
EcoNI 1021 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2731 CCTGCCAAAGG
EcoT22I 1784 ATGCAT ATGCA^T
5479 ATGCAT
HhaI 3252 GCGC GCG^C
3725 GCGC
HinP1I 3252 GCGC G^CGC
3725 GCGC
HincII 4905 GTTGAC GT[CT]^[GA]AC
4999 GTTAAC
HindII 4905 GTTGAC GT[CT]^[GA]AC
4999 GTTAAC
MluNI 2960 TGGCCA TGG^CCA
4282 TGGCCA
MscI 2960 TGGCCA TGG^CCA
4282 TGGCCA
NsiI 1784 ATGCAT ATGCA^T
5479 ATGCAT
PshBI 5280 ATTAAT AT^TAAT
6262 ATTAAT
SphI 3185 GCATGC GCATG^C
6238 GCATGC
StuI 136 AGGCCT AGG^CCT
2728 AGGCCT
XmnI 1334 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4459 GAAACCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI AspHI AvaI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaBI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI EarI Eco81I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII