##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       AccII       AcyI        AgeI        AhdI        
ApaI        AscI        AspEI       AviII       BbeI        
BbrPI       BciVI       BclI        BglI        BglII       
BlpI        Bpu1102I    BsaAI       BsaHI       BsiEI       
BsiWI       BspHI       BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       Cfr10I      CpoI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoO109I    EcoO65I     FbaI        
FseI        FspI        HgaI        Hin1I       KasI        
KspI        MfeI        MluI        MunI        MvnI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       Sse8387I    XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       2140  GGTACC               G^GTACC
AccI         2036  GTAGAC               GT^[AC][GT]AC
AccIII       1804  TCCGGA               T^CCGGA
AclI         4555  AACGTT               AA^CGTT
Aor51HI      3251  AGCGCT               AGC^GCT
Asp718I      2140  GGTACC               G^GTACC
AspI         3686  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         351  GGACC                G^G[AT]CC
AvrII        3636  CCTAGG               C^CTAGG
BanII        1623  GGGCTC               G[GA]GC[CT]^C
BcgI         3768  CGATTCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         3636  CCTAGG               C^CTAGG
BsaWI        1804  TCCGGA               [AT]^CCGG[AT]
BseAI        1804  TCCGGA               T^CCGGA
BseRI        5309  CTCCTC               GAGGAG(10/8)
Bsp1407I      691  TGTACA               T^GTACA
BspDI        2454  ATCGAT               AT^CGAT
BspEI        1804  TCCGGA               T^CCGGA
BsrGI         691  TGTACA               T^GTACA
BstXI         834  CCATCCAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI         2454  ATCGAT               AT^CGAT
Eco47III     3251  AGCGCT               AGC^GCT
EcoRV        5856  GATATC               GAT^ATC
FauI         1758  CCCGC                CCCGC(4/6)
HaeII        3251  AGCGCT               [GA]GCGC^[CT]
HpaI         4999  GTTAAC               GTT^AAC
KpnI         2140  GGTACC               GGTAC^C
MroI         1804  TCCGGA               T^CCGGA
NcoI         1107  CCATGG               C^CATGG
NdeI         3303  CATATG               CA^TATG
PflFI        3686  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I     4555  AACGTT               AA^CGTT
SapI         1856  GAAGAGC              GCTCTTC(1/4)
SmaI         3756  CCCGGG               CCC^GGG
SpeI         2631  ACTAGT               A^CTAGT
SspBI         691  TGTACA               T^GTACA
SwaI         3569  ATTTAAAT             ATTT^AAAT
Tth111I      3686  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4304  TCTAGA               T^CTAGA
XmaCI        3756  CCCGGG               C^CCGGG
XmaI         3756  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AflII        4820  CTTAAG               C^TTAAG
             6243  CTTAAG              
AseI         5280  ATTAAT               AT^TAAT
             6262  ATTAAT              
AsnI         5280  ATTAAT               AT^TAAT
             6262  ATTAAT              
Asp700I      1334  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4459  GAAACCATTC          
BalI         2960  TGGCCA               TGG^CCA
             4282  TGGCCA              
BamHI        1637  GGATCC               G^GATCC
             3358  GGATCC              
BanI         2140  GGTACC               G^G[CT][GA]CC
             6429  GGCACC              
BbvCI        1080  CCTCAGC              CCTCAGC(-5/-2)
             3779  CCTCAGC             
BfrI         4820  CTTAAG               C^TTAAG
             6243  CTTAAG              
BsgI         2553  GTGCAG               GTGCAG(16/14)
             5532  GTGCAG              
BsmBI        1312  CGTCTC               CGTCTC(1/5)
             1575  GAGACG              
BsmFI         256  GGGAC                GGGAC(10/14)
             3799  GGGAC               
BsmI         1766  GAATGC               GAATGC(1/-1)
             2428  GCATTC              
BstAPI       1761  GCAGTGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3743  GCAACTTCTGC         
CfoI         3252  GCGC                 GCG^C
             3725  GCGC                
DsaI         1107  CCATGG               C^C[GA][CT]GG
             1642  CCACGG              
EaeI         2960  TGGCCA               [CT]^GGCC[GA]
             4282  TGGCCA              
EcoNI        1021  CCTCAGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2731  CCTGCCAAAGG         
EcoT22I      1784  ATGCAT               ATGCA^T
             5479  ATGCAT              
HhaI         3252  GCGC                 GCG^C
             3725  GCGC                
HinP1I       3252  GCGC                 G^CGC
             3725  GCGC                
HincII       4905  GTTGAC               GT[CT]^[GA]AC
             4999  GTTAAC              
HindII       4905  GTTGAC               GT[CT]^[GA]AC
             4999  GTTAAC              
MluNI        2960  TGGCCA               TGG^CCA
             4282  TGGCCA              
MscI         2960  TGGCCA               TGG^CCA
             4282  TGGCCA              
NsiI         1784  ATGCAT               ATGCA^T
             5479  ATGCAT              
PshBI        5280  ATTAAT               AT^TAAT
             6262  ATTAAT              
SphI         3185  GCATGC               GCATG^C
             6238  GCATGC              
StuI          136  AGGCCT               AGG^CCT
             2728  AGGCCT              
XmnI         1334  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4459  GAAACCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
Alw44I      AlwI        AlwNI       AocI        ApaLI       
ApoI        AspHI       AvaI        BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaBI       BsaI        BsaJI       BsiHKAI     BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BspLU11I    
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Bsu36I      Cac8I       Cfr13I      
DdeI        DpnII       DraI        EarI        Eco81I      
EcoRI       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII