##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII ApoI AscI AseI AsnI
Asp718I AviII BbrPI BciVI BclI
BfrI BsaAI BsaBI BseAI BsiEI
BsmBI BsmI BspDI BspEI BspHI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MluI MroI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfuI SnaBI SpeI
Sse8387I SspI SwaI TfiI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 333 CGCG CG^CG
AgeI 1633 ACCGGT A^CCGGT
AhdI 2776 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2776 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1378 CCTAGG C^CTAGG
BamHI 1236 GGATCC G^GATCC
BbeI 1645 GGCGCC GGCGC^C
BbsI 152 GAAGAC GAAGAC(2/6)
BcgI 1921 CGACACTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1378 CCTAGG C^CTAGG
BlpI 2877 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2877 GCTAAGC GC^T[ACGT]AGC
BpuAI 152 GAAGAC GAAGAC(2/6)
BsaWI 1633 ACCGGT [AT]^CCGG[AT]
BsiWI 2216 CGTACG C^GTACG
BspLU11I 1835 ACATGT A^CATGT
BstAPI 404 GCACGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 333 CGCG CG^CG
CelII 2877 GCTAAGC GC^T[ACGT]AGC
DraI 3016 TTTAAA TTT^AAA
Eam1105I 2776 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KasI 1645 GGCGCC G^GCGCC
MvnI 333 CGCG CG^CG
NarI 1645 GGCGCC GG^CGCC
NheI 1750 GCTAGC G^CTAGC
PinAI 1633 ACCGGT A^CCGGT
SacI 2800 GAGCTC GAGCT^C
SapI 476 GCTCTTC GCTCTTC(1/4)
ScaI 133 AGTACT AGT^ACT
SexAI 1251 ACCAGGT A^CC[AT]GGT
SfiI 2602 GGCCTGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1645 GGCGCC GGC^GCC
SphI 3464 GCATGC GCATG^C
TaqI 139 TCGA T^CGA
TthHB8I 139 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1049 GTAGAC GT^[AC][GT]AC
2211 GTAGAC
AcyI 360 GGCGTC G[GA]^CG[CT]C
1645 GGCGCC
AflIII 271 ACGTGT A^C[GA][CT]GT
1835 ACATGT
Alw44I 544 GTGCAC G^TGCAC
1629 GTGCAC
ApaLI 544 GTGCAC G^TGCAC
1629 GTGCAC
Asp700I 2 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
323 GAAGTGCTTC
AspI 357 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1052 GACCCAGTC
BbvCI 945 CCTCAGC CCTCAGC(-5/-2)
2958 CCTCAGC
BglI 2603 GCCTGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3175 GCCTGTTTGGC
BglII 187 AGATCT A^GATCT
3137 AGATCT
BsaHI 360 GGCGTC G[GA]^CG[CT]C
1645 GGCGCC
BseRI 679 GAGGAG GAGGAG(10/8)
1041 GAGGAG
BsgI 536 CTGCAC GTGCAG(16/14)
1247 CTGCAC
BsrBI 474 CCGCTC CCGCTC(-3/-3)
1547 CCGCTC
BsrDI 25 GCAATG GCAATG(2/0)
2681 GCAATG
BssSI 3419 CTCGTG CACGAG(-5/-1)
3505 CTCGTG
BstXI 2016 CCATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3296 CCACAGTTCTGG
DraIII 1157 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1250 CACCAGGTG
Hin1I 360 GGCGTC G[GA]^CG[CT]C
1645 GGCGCC
MaeII 271 ACGT A^CGT
2215 ACGT
MfeI 1730 CAATTG C^AATTG
2194 CAATTG
MslI 608 CACAGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3465 CATGCGAGTG
MunI 1730 CAATTG C^AATTG
2194 CAATTG
NaeI 211 GCCGGC GCC^GGC
351 GCCGGC
NcoI 2016 CCATGG C^CATGG
2512 CCATGG
NgoMIV 211 GCCGGC G^CCGGC
351 GCCGGC
PflFI 357 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1052 GACCCAGTC
PstI 850 CTGCAG CTGCA^G
1141 CTGCAG
SfaNI 70 GCATC GCATC(5/9)
722 GCATC
SgrAI 231 CACCGGCG C[GA]^CCGG[CT]G
1632 CACCGGTG
SmlI 306 CTCAAG C^T[CT][GA]AG
3282 CTCAAG
Tth111I 357 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1052 GACCCAGTC
XmnI 2 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
323 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI Aor51HI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
SmaI SspBI StuI StyI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII XmaCI XmaI