##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AhdI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BlnI BsaBI BsiWI BspDI
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI Eam1105I EcoRI
FseI FspI HindIII HpaI KasI
KpnI MamI MfeI MluI MunI
NarI NdeI NotI NruI NspV
PacI PmeI PshBI PvuI RsrII
SbfI ScaI SexAI SfoI SfuI
SgrAI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2778 GACGTC GACGT^C
AclI 3648 AACGTT AA^CGTT
AcyI 2778 GACGTC G[GA]^CG[CT]C
AflII 4683 CTTAAG C^TTAAG
Aor51HI 237 AGCGCT AGC^GCT
BbrPI 2131 CACGTG CAC^GTG
BclI 3839 TGATCA T^GATCA
BfrI 4683 CTTAAG C^TTAAG
BglII 618 AGATCT A^GATCT
BsaHI 2778 GACGTC G[GA]^CG[CT]C
BsmBI 2776 GAGACG CGTCTC(1/5)
BspHI 2354 TCATGA T^CATGA
BspLU11I 4717 ACATGT A^CATGT
BsrDI 3320 GCAATG GCAATG(2/0)
BstEII 5031 GGTCACC G^GT[ACGT]ACC
BstPI 5031 GGTCACC G^GT[ACGT]ACC
DraIII 5717 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1692 GACCAACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 237 AGCGCT AGC^GCT
EcoO65I 5031 GGTCACC G^GT[ACGT]ACC
EcoRV 2161 GATATC GAT^ATC
FbaI 3839 TGATCA T^GATCA
Hin1I 2778 GACGTC G[GA]^CG[CT]C
HincII 3105 GTCGAC GT[CT]^[GA]AC
HindII 3105 GTCGAC GT[CT]^[GA]AC
NaeI 1346 GCCGGC GCC^GGC
NgoMIV 1346 GCCGGC G^CCGGC
NheI 271 GCTAGC G^CTAGC
PmaCI 2131 CACGTG CAC^GTG
PmlI 2131 CACGTG CAC^GTG
PshAI 5269 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3648 AACGTT AA^CGTT
RcaI 2354 TCATGA T^CATGA
SalI 3105 GTCGAC G^TCGAC
SnaBI 5435 TACGTA TAC^GTA
SphI 3349 GCATGC GCATG^C
StuI 1316 AGGCCT AGG^CCT
XbaI 3659 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 21 TCCGGA T^CCGGA
1268 TCCGGA
AflIII 4717 ACATGT A^C[GA][CT]GT
5076 ACGTGT
AgeI 4373 ACCGGT A^CCGGT
4490 ACCGGT
ApaI 1962 GGGCCC GGGCC^C
2737 GGGCCC
BcgI 261 CGAGGGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2773 GCAGAGACGTCG
BciVI 2608 GGATAC GTATCC(6/5)
5553 GGATAC
BsaI 934 GAGACC GGTCTC(1/5)
3071 GAGACC
BseAI 21 TCCGGA T^CCGGA
1268 TCCGGA
BsiEI 169 CGGCCG CG[GA][CT]^CG
1539 CGGCCG
Bsp1407I 2621 TGTACA T^GTACA
5364 TGTACA
BspEI 21 TCCGGA T^CCGGA
1268 TCCGGA
BsrGI 2621 TGTACA T^GTACA
5364 TGTACA
BstAPI 421 GCACCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
486 GCACGCCTTGC
BstXI 866 CCAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2722 CCATCCCACTGG
EagI 169 CGGCCG C^GGCCG
1539 CGGCCG
EclXI 169 CGGCCG C^GGCCG
1539 CGGCCG
Eco52I 169 CGGCCG C^GGCCG
1539 CGGCCG
MroI 21 TCCGGA T^CCGGA
1268 TCCGGA
NcoI 872 CCATGG C^CATGG
4837 CCATGG
PaeR7I 3054 CTCGAG C^TCGAG
5571 CTCGAG
PinAI 4373 ACCGGT A^CCGGT
4490 ACCGGT
PstI 146 CTGCAG CTGCA^G
996 CTGCAG
PvuII 547 CAGCTG CAG^CTG
2529 CAGCTG
SapI 253 GCTCTTC GCTCTTC(1/4)
1705 GCTCTTC
SfiI 840 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1800 GGCCCGGGCGGCC
SmaI 817 CCCGGG CCC^GGG
1802 CCCGGG
SspBI 2621 TGTACA T^GTACA
5364 TGTACA
XhoI 3054 CTCGAG C^TCGAG
5571 CTCGAG
XmaCI 817 CCCGGG C^CCGGG
1802 CCCGGG
XmaI 817 CCCGGG C^CCGGG
1802 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI Asp700I AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflFI PflMI
PleI PpuMI RsaI SacI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmnI