################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AclI AcsI AflII AhdI ApoI AseI AsnI Asp700I AspEI BciVI BclI BfrI BsaBI BsiWI BsmI BspHI Bst1107I BstBI BstZ17I CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FbaI HindIII HpaI KspI MamI MluI NdeI NotI NruI NsiI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII ScaI SexAI SfuI SgrAI SnaBI SpeI SspI SwaI TfiI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 1374 GACGTC GACGT^C AgeI 4864 ACCGGT A^CCGGT AscI 3217 GGCGCGCC GG^CGCGCC AspI 2469 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2092 TGCGCA TGC^GCA AvrII 4936 CCTAGG C^CTAGG BamHI 4159 GGATCC G^GATCC BbeI 396 GGCGCC GGCGC^C BbrPI 1496 CACGTG CAC^GTG BbvCI 864 GCTGAGG CCTCAGC(-5/-2) BglII 5046 AGATCT A^GATCT BlnI 4936 CCTAGG C^CTAGG BsaAI 1496 CACGTG [CT]AC^GT[GA] BsmBI 534 GAGACG CGTCTC(1/5) BspDI 2310 ATCGAT AT^CGAT BspLU11I 2374 ACATGT A^CATGT BssHII 3218 GCGCGC G^CGCGC ClaI 2310 ATCGAT AT^CGAT DrdI 2466 GACGACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 73 CGGCCG C^GGCCG EclXI 73 CGGCCG C^GGCCG Eco52I 73 CGGCCG C^GGCCG FseI 986 GGCCGGCC GGCCGG^CC FspI 2092 TGCGCA TGC^GCA KasI 396 GGCGCC G^GCGCC MfeI 639 CAATTG C^AATTG MunI 639 CAATTG C^AATTG NarI 396 GGCGCC GG^CGCC NheI 4673 GCTAGC G^CTAGC PflFI 2469 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 4864 ACCGGT A^CCGGT PmaCI 1496 CACGTG CAC^GTG PmlI 1496 CACGTG CAC^GTG SalI 2651 GTCGAC G^TCGAC SfoI 396 GGCGCC GGC^GCC Tsp509I 640 AATT ^AATT Tth111I 2469 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Acc65I 2178 GGTACC G^GTACC 2491 GGTACC AccI 2651 GTCGAC GT^[AC][GT]AC 3291 GTCTAC AccIII 508 TCCGGA T^CCGGA 790 TCCGGA AflIII 1495 ACACGT A^C[GA][CT]GT 2374 ACATGT Asp718I 2178 GGTACC G^GTACC 2491 GGTACC BsaI 625 GAGACC GGTCTC(1/5) 4133 GGTCTC BseAI 508 TCCGGA T^CCGGA 790 TCCGGA Bsp1407I 2200 TGTACA T^GTACA 2995 TGTACA BspEI 508 TCCGGA T^CCGGA 790 TCCGGA BsrGI 2200 TGTACA T^GTACA 2995 TGTACA BstEII 4298 GGTGACC G^GT[ACGT]ACC 4571 GGTGACC BstPI 4298 GGTGACC G^GT[ACGT]ACC 4571 GGTGACC EcoO65I 4298 GGTGACC G^GT[ACGT]ACC 4571 GGTGACC HincII 1377 GTCAAC GT[CT]^[GA]AC 2651 GTCGAC HindII 1377 GTCAAC GT[CT]^[GA]AC 2651 GTCGAC KpnI 2178 GGTACC GGTAC^C 2491 GGTACC MroI 508 TCCGGA T^CCGGA 790 TCCGGA MseI 5068 TTAA T^TAA 5083 TTAA SphI 1142 GCATGC GCATG^C 3835 GCATGC SspBI 2200 TGTACA T^GTACA 2995 TGTACA Tru9I 5068 TTAA T^TAA 5083 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SfiI SmaI SmlI Sse8387I StuI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI