##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AclI        AcsI        AflII       AhdI        ApoI        
AseI        AsnI        Asp700I     AspEI       BciVI       
BclI        BfrI        BsaBI       BsiWI       BsmI        
BspHI       Bst1107I    BstBI       BstZ17I     CpoI        
DraI        Eam1105I    EcoRI       EcoRV       EcoT22I     
FbaI        HindIII     HpaI        KspI        MamI        
MluI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       ScaI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        SspI        SwaI        TfiI        
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        1374  GACGTC               GACGT^C
AgeI         4864  ACCGGT               A^CCGGT
AscI         3217  GGCGCGCC             GG^CGCGCC
AspI         2469  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2092  TGCGCA               TGC^GCA
AvrII        4936  CCTAGG               C^CTAGG
BamHI        4159  GGATCC               G^GATCC
BbeI          396  GGCGCC               GGCGC^C
BbrPI        1496  CACGTG               CAC^GTG
BbvCI         864  GCTGAGG              CCTCAGC(-5/-2)
BglII        5046  AGATCT               A^GATCT
BlnI         4936  CCTAGG               C^CTAGG
BsaAI        1496  CACGTG               [CT]AC^GT[GA]
BsmBI         534  GAGACG               CGTCTC(1/5)
BspDI        2310  ATCGAT               AT^CGAT
BspLU11I     2374  ACATGT               A^CATGT
BssHII       3218  GCGCGC               G^CGCGC
ClaI         2310  ATCGAT               AT^CGAT
DrdI         2466  GACGACTTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           73  CGGCCG               C^GGCCG
EclXI          73  CGGCCG               C^GGCCG
Eco52I         73  CGGCCG               C^GGCCG
FseI          986  GGCCGGCC             GGCCGG^CC
FspI         2092  TGCGCA               TGC^GCA
KasI          396  GGCGCC               G^GCGCC
MfeI          639  CAATTG               C^AATTG
MunI          639  CAATTG               C^AATTG
NarI          396  GGCGCC               GG^CGCC
NheI         4673  GCTAGC               G^CTAGC
PflFI        2469  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        4864  ACCGGT               A^CCGGT
PmaCI        1496  CACGTG               CAC^GTG
PmlI         1496  CACGTG               CAC^GTG
SalI         2651  GTCGAC               G^TCGAC
SfoI          396  GGCGCC               GGC^GCC
Tsp509I       640  AATT                 ^AATT
Tth111I      2469  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Acc65I       2178  GGTACC               G^GTACC
             2491  GGTACC              
AccI         2651  GTCGAC               GT^[AC][GT]AC
             3291  GTCTAC              
AccIII        508  TCCGGA               T^CCGGA
              790  TCCGGA              
AflIII       1495  ACACGT               A^C[GA][CT]GT
             2374  ACATGT              
Asp718I      2178  GGTACC               G^GTACC
             2491  GGTACC              
BsaI          625  GAGACC               GGTCTC(1/5)
             4133  GGTCTC              
BseAI         508  TCCGGA               T^CCGGA
              790  TCCGGA              
Bsp1407I     2200  TGTACA               T^GTACA
             2995  TGTACA              
BspEI         508  TCCGGA               T^CCGGA
              790  TCCGGA              
BsrGI        2200  TGTACA               T^GTACA
             2995  TGTACA              
BstEII       4298  GGTGACC              G^GT[ACGT]ACC
             4571  GGTGACC             
BstPI        4298  GGTGACC              G^GT[ACGT]ACC
             4571  GGTGACC             
EcoO65I      4298  GGTGACC              G^GT[ACGT]ACC
             4571  GGTGACC             
HincII       1377  GTCAAC               GT[CT]^[GA]AC
             2651  GTCGAC              
HindII       1377  GTCAAC               GT[CT]^[GA]AC
             2651  GTCGAC              
KpnI         2178  GGTACC               GGTAC^C
             2491  GGTACC              
MroI          508  TCCGGA               T^CCGGA
              790  TCCGGA              
MseI         5068  TTAA                 T^TAA
             5083  TTAA                
SphI         1142  GCATGC               GCATG^C
             3835  GCATGC              
SspBI        2200  TGTACA               T^GTACA
             2995  TGTACA              
Tru9I        5068  TTAA                 T^TAA
             5083  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (  142 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcgI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaJI       BsaWI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        Eco47III    Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      SbfI        ScrFI       SfaNI       
SfcI        SfiI        SmaI        SmlI        Sse8387I    
StuI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI