##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI Aor51HI ApaI AscI Asp718I
AvaI AvrII BamHI BbeI BbrPI
BciVI BcnI BglI BlnI BsaBI
BsaHI BsaI BsiEI BsiWI BsmBI
BsoBI BspDI BsrFI BssHII BstBI
BstUI Cfr10I ClaI CpoI EagI
EclXI Eco47III Eco52I FauI FseI
HaeII HgaI Hin1I KasI KpnI
KspI MamI MluI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 3041 TCCGGA T^CCGGA
AflII 6404 CTTAAG C^TTAAG
AhdI 6453 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3191 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 5460 CCTCAGG CC^T[ACGT]AGG
AspEI 6453 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1385 TGCGCA TGC^GCA
BanII 7144 GAGCTC G[GA]GC[CT]^C
BbvCI 6157 CCTCAGC CCTCAGC(-5/-2)
BcgI 2742 GCAACTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 6404 CTTAAG C^TTAAG
BglII 3175 AGATCT A^GATCT
BlpI 5232 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 5232 GCTAAGC GC^T[ACGT]AGC
BsaWI 3041 TCCGGA [AT]^CCGG[AT]
BseAI 3041 TCCGGA T^CCGGA
BseRI 6896 CTCCTC GAGGAG(10/8)
BspEI 3041 TCCGGA T^CCGGA
BspMI 1756 ACCTGC ACCTGC(4/8)
BsrBI 3098 CCGCTC CCGCTC(-3/-3)
BstAPI 958 GCAGATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2200 GGTGACC G^GT[ACGT]ACC
BstPI 2200 GGTGACC G^GT[ACGT]ACC
Bsu36I 5460 CCTCAGG CC^T[ACGT]AGG
CelII 5232 GCTAAGC GC^T[ACGT]AGC
DraIII 5336 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3745 GACATTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 6453 GACATTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5460 CCTCAGG CC^T[ACGT]AGG
EcoNI 1372 CCTTGGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2200 GGTGACC G^GT[ACGT]ACC
EcoRI 5656 GAATTC G^AATTC
EcoRV 763 GATATC GAT^ATC
FspI 1385 TGCGCA TGC^GCA
HapII 3042 CCGG C^CGG
HpaI 463 GTTAAC GTT^AAC
HpaII 3042 CCGG C^CGG
MroI 3041 TCCGGA T^CCGGA
MspI 3042 CCGG C^CGG
SacI 7144 GAGCTC GAGCT^C
SpeI 358 ACTAGT A^CTAGT
StuI 2964 AGGCCT AGG^CCT
SwaI 3600 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Alw44I 2115 GTGCAC G^TGCAC
6074 GTGCAC
ApaLI 2115 GTGCAC G^TGCAC
6074 GTGCAC
BbsI 1417 GAAGAC GAAGAC(2/6)
4158 GTCTTC
BpuAI 1417 GAAGAC GAAGAC(2/6)
4158 GTCTTC
BsaAI 2421 TACGTG [CT]AC^GT[GA]
6564 CACGTA
BspLU11I 1421 ACATGT A^CATGT
2260 ACATGT
BssSI 2927 CACGAG CACGAG(-5/-1)
4171 CACGAG
CfoI 1386 GCGC GCG^C
4176 GCGC
HhaI 1386 GCGC GCG^C
4176 GCGC
HinP1I 1386 GCGC G^CGC
4176 GCGC
NcoI 4221 CCATGG C^CATGG
4592 CCATGG
PpuMI 4477 AGGTCCT [GA]G^G[AT]CC[CT]
6248 GGGACCT
SapI 4127 GAAGAGC GCTCTTC(1/4)
7286 GCTCTTC
ScaI 4503 AGTACT AGT^ACT
6881 AGTACT
SphI 4587 GCATGC GCATG^C
7176 GCATGC
XbaI 1737 TCTAGA T^CTAGA
6836 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AspI AvaII BalI
BanI BbvI BclI BfaI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BsrDI BsrGI BsrI
BssKI Bst1107I BstF5I BstNI BstXI
BstYI BstZ17I Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I EcoT22I
FbaI Fnu4HI FokI HaeIII HincII
HindII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MunI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NsiI NspI PflFI PflMI
PleI PshBI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII XmnI