##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

AatII       AccIII      AgeI        AscI        AspI        
AviII       AvrII       BbvCI       BciVI       BlnI        
BseAI       BsiWI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrGI       BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        DrdI        EcoRI       EcoRV       
FseI        FspI        HindIII     MluI        MroI        
NheI        NotI        NspV        PacI        PflFI       
PinAI       PmeI        SalI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SnaBI       SphI        
Sse8387I    SspBI       Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AclI         2010  AACGTT               AA^CGTT
AflII        5181  CTTAAG               C^TTAAG
AseI         3869  ATTAAT               AT^TAAT
AsnI         3869  ATTAAT               AT^TAAT
BamHI        2576  GGATCC               G^GATCC
BbrPI        5681  CACGTG               CAC^GTG
BbsI         2838  GTCTTC               GAAGAC(2/6)
BcgI         3144  GCAGGAGAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         5181  CTTAAG               C^TTAAG
BpuAI        2838  GTCTTC               GAAGAC(2/6)
BsaAI        5681  CACGTG               [CT]AC^GT[GA]
BsaWI        3263  ACCGGA               [AT]^CCGG[AT]
BspHI        4951  TCATGA               T^CATGA
BssHII       1186  GCGCGC               G^CGCGC
BstAPI       5786  GCATTGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI         1178  CGGACCG              CG^G[AT]CCG
EcoT22I      2033  ATGCAT               ATGCA^T
HpaI         4054  GTTAAC               GTT^AAC
MfeI         3559  CAATTG               C^AATTG
MunI         3559  CAATTG               C^AATTG
NaeI          260  GCCGGC               GCC^GGC
NdeI         3672  CATATG               CA^TATG
NgoMIV        260  GCCGGC               G^CCGGC
NsiI         2033  ATGCAT               ATGCA^T
PaeR7I       2216  CTCGAG               C^TCGAG
PmaCI        5681  CACGTG               CAC^GTG
PmlI         5681  CACGTG               CAC^GTG
PshAI        3318  GACTCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3869  ATTAAT               AT^TAAT
Psp1406I     2010  AACGTT               AA^CGTT
PvuI         2253  CGATCG               CGAT^CG
RcaI         4951  TCATGA               T^CATGA
RsrII        1178  CGGACCG              CG^G[AT]CCG
SacI         5421  GAGCTC               GAGCT^C
SapI         3540  GCTCTTC              GCTCTTC(1/4)
SexAI        6282  ACCAGGT              A^CC[AT]GGT
SpeI         3936  ACTAGT               A^CTAGT
SwaI         4715  ATTTAAAT             ATTT^AAAT
XcmI         4929  CCACTCCAACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2216  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Acc65I       3260  GGTACC               G^GTACC
             5844  GGTACC              
Alw44I       1876  GTGCAC               G^TGCAC
             5494  GTGCAC              
AocI           13  CCTAAGG              CC^T[ACGT]AGG
             5774  CCTTAGG             
Aor51HI      1329  AGCGCT               AGC^GCT
             3336  AGCGCT              
ApaLI        1876  GTGCAC               G^TGCAC
             5494  GTGCAC              
Asp700I      3697  GAAATGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             6218  GAAGTGCTTC          
Asp718I      3260  GGTACC               G^GTACC
             5844  GGTACC              
BclI         1766  TGATCA               T^GATCA
             3863  TGATCA              
BglII        3344  AGATCT               A^GATCT
             5536  AGATCT              
BsaBI        1689  GATTGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1761  GATTGTGATC          
BsmBI        1866  CGTCTC               CGTCTC(1/5)
             3183  CGTCTC              
BsrDI        2277  CATTGC               GCAATG(2/0)
             6818  GCAATG              
BstEII       2913  GGTAACC              G^GT[ACGT]ACC
             3437  GGTTACC             
BstPI        2913  GGTAACC              G^GT[ACGT]ACC
             3437  GGTTACC             
BstXI          92  CCAGGTCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              194  CCACGTCCCTGG        
Bsu36I         13  CCTAAGG              CC^T[ACGT]AGG
             5774  CCTTAGG             
Eco47III     1329  AGCGCT               AGC^GCT
             3336  AGCGCT              
Eco81I         13  CCTAAGG              CC^T[ACGT]AGG
             5774  CCTTAGG             
EcoO65I      2913  GGTAACC              G^GT[ACGT]ACC
             3437  GGTTACC             
FbaI         1766  TGATCA               T^GATCA
             3863  TGATCA              
KpnI         3260  GGTACC               GGTAC^C
             5844  GGTACC              
MamI         1689  GATTGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1761  GATTGTGATC          
NruI          573  TCGCGA               TCG^CGA
              869  TCGCGA              
PflMI        2282  CCAACCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3906  CCACATCATGG         
SspI         1951  AATATT               AAT^ATT
             4386  AATATT              
Van91I       2282  CCAACCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3906  CCACATCATGG         
XmnI         3697  GAAATGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             6218  GAAGTGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  142 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AhdI        AluI        AlwI        
AlwNI       ApaI        ApoI        AspEI       AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco52I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        PvuII       RsaI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmaCI       XmaI