################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AatII AccIII AgeI AscI AspI AviII AvrII BbvCI BciVI BlnI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssSI Bst1107I BstBI BstZ17I ClaI DrdI EcoRI EcoRV FseI FspI HindIII MluI MroI NheI NotI NspV PacI PflFI PinAI PmeI SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspBI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AclI 2010 AACGTT AA^CGTT AflII 5181 CTTAAG C^TTAAG AseI 3869 ATTAAT AT^TAAT AsnI 3869 ATTAAT AT^TAAT BamHI 2576 GGATCC G^GATCC BbrPI 5681 CACGTG CAC^GTG BbsI 2838 GTCTTC GAAGAC(2/6) BcgI 3144 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 5181 CTTAAG C^TTAAG BpuAI 2838 GTCTTC GAAGAC(2/6) BsaAI 5681 CACGTG [CT]AC^GT[GA] BsaWI 3263 ACCGGA [AT]^CCGG[AT] BspHI 4951 TCATGA T^CATGA BssHII 1186 GCGCGC G^CGCGC BstAPI 5786 GCATTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CpoI 1178 CGGACCG CG^G[AT]CCG EcoT22I 2033 ATGCAT ATGCA^T HpaI 4054 GTTAAC GTT^AAC MfeI 3559 CAATTG C^AATTG MunI 3559 CAATTG C^AATTG NaeI 260 GCCGGC GCC^GGC NdeI 3672 CATATG CA^TATG NgoMIV 260 GCCGGC G^CCGGC NsiI 2033 ATGCAT ATGCA^T PaeR7I 2216 CTCGAG C^TCGAG PmaCI 5681 CACGTG CAC^GTG PmlI 5681 CACGTG CAC^GTG PshAI 3318 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3869 ATTAAT AT^TAAT Psp1406I 2010 AACGTT AA^CGTT PvuI 2253 CGATCG CGAT^CG RcaI 4951 TCATGA T^CATGA RsrII 1178 CGGACCG CG^G[AT]CCG SacI 5421 GAGCTC GAGCT^C SapI 3540 GCTCTTC GCTCTTC(1/4) SexAI 6282 ACCAGGT A^CC[AT]GGT SpeI 3936 ACTAGT A^CTAGT SwaI 4715 ATTTAAAT ATTT^AAAT XcmI 4929 CCACTCCAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2216 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 3260 GGTACC G^GTACC 5844 GGTACC Alw44I 1876 GTGCAC G^TGCAC 5494 GTGCAC AocI 13 CCTAAGG CC^T[ACGT]AGG 5774 CCTTAGG Aor51HI 1329 AGCGCT AGC^GCT 3336 AGCGCT ApaLI 1876 GTGCAC G^TGCAC 5494 GTGCAC Asp700I 3697 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6218 GAAGTGCTTC Asp718I 3260 GGTACC G^GTACC 5844 GGTACC BclI 1766 TGATCA T^GATCA 3863 TGATCA BglII 3344 AGATCT A^GATCT 5536 AGATCT BsaBI 1689 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1761 GATTGTGATC BsmBI 1866 CGTCTC CGTCTC(1/5) 3183 CGTCTC BsrDI 2277 CATTGC GCAATG(2/0) 6818 GCAATG BstEII 2913 GGTAACC G^GT[ACGT]ACC 3437 GGTTACC BstPI 2913 GGTAACC G^GT[ACGT]ACC 3437 GGTTACC BstXI 92 CCAGGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 194 CCACGTCCCTGG Bsu36I 13 CCTAAGG CC^T[ACGT]AGG 5774 CCTTAGG Eco47III 1329 AGCGCT AGC^GCT 3336 AGCGCT Eco81I 13 CCTAAGG CC^T[ACGT]AGG 5774 CCTTAGG EcoO65I 2913 GGTAACC G^GT[ACGT]ACC 3437 GGTTACC FbaI 1766 TGATCA T^GATCA 3863 TGATCA KpnI 3260 GGTACC GGTAC^C 5844 GGTACC MamI 1689 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1761 GATTGTGATC NruI 573 TCGCGA TCG^CGA 869 TCGCGA PflMI 2282 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3906 CCACATCATGG SspI 1951 AATATT AAT^ATT 4386 AATATT Van91I 2282 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3906 CCACATCATGG XmnI 3697 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6218 GAAGTGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AhdI AluI AlwI AlwNI ApaI ApoI AspEI AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI