##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AscI AspEI AviII BbrPI BcgI
BciVI BfrI BglII BsaBI BsiWI
BsmI BspDI BspHI BspLU11I Bst1107I
BstBI BstZ17I ClaI CpoI Eam1105I
EcoRI EcoT22I FseI FspI HpaI
KspI MamI MluI NdeI NheI
NotI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
RcaI RsrII SacII SexAI SfiI
SfuI SgrAI SnaBI SpeI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 4422 GGTACC G^GTACC
AccI 368 GTCGAC GT^[AC][GT]AC
AccIII 1065 TCCGGA T^CCGGA
AflIII 4434 ACACGT A^C[GA][CT]GT
AseI 5699 ATTAAT AT^TAAT
AsnI 5699 ATTAAT AT^TAAT
Asp718I 4422 GGTACC G^GTACC
AvrII 3147 CCTAGG C^CTAGG
BamHI 3193 GGATCC G^GATCC
BclI 5673 TGATCA T^GATCA
BlnI 3147 CCTAGG C^CTAGG
BseAI 1065 TCCGGA T^CCGGA
Bsp1407I 60 TGTACA T^GTACA
BspEI 1065 TCCGGA T^CCGGA
BsrGI 60 TGTACA T^GTACA
BstEII 2763 GGTGACC G^GT[ACGT]ACC
BstPI 2763 GGTGACC G^GT[ACGT]ACC
EcoO65I 2763 GGTGACC G^GT[ACGT]ACC
EcoRV 2054 GATATC GAT^ATC
FbaI 5673 TGATCA T^GATCA
HincII 368 GTCGAC GT[CT]^[GA]AC
HindII 368 GTCGAC GT[CT]^[GA]AC
HindIII 6155 AAGCTT A^AGCTT
KpnI 4422 GGTACC GGTAC^C
MroI 1065 TCCGGA T^CCGGA
NruI 1848 TCGCGA TCG^CGA
PshAI 361 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5699 ATTAAT AT^TAAT
PvuI 549 CGATCG CGAT^CG
SalI 368 GTCGAC G^TCGAC
SbfI 2769 CCTGCAGG CCTGCA^GG
ScaI 2701 AGTACT AGT^ACT
SphI 888 GCATGC GCATG^C
Sse8387I 2769 CCTGCAGG CCTGCA^GG
SspBI 60 TGTACA T^GTACA
SspI 5940 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Aor51HI 1392 AGCGCT AGC^GCT
3080 AGCGCT
Asp700I 1657 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3992 GAAGGCCTTC
BbeI 1403 GGCGCC GGCGC^C
1732 GGCGCC
BsaAI 1181 TACGTG [CT]AC^GT[GA]
3874 CACGTA
BsaWI 1065 TCCGGA [AT]^CCGG[AT]
2278 ACCGGA
BsrDI 489 GCAATG GCAATG(2/0)
2391 GCAATG
DraI 4906 TTTAAA TTT^AAA
5706 TTTAAA
EagI 2990 CGGCCG C^GGCCG
3415 CGGCCG
EclXI 2990 CGGCCG C^GGCCG
3415 CGGCCG
Eco47III 1392 AGCGCT AGC^GCT
3080 AGCGCT
Eco52I 2990 CGGCCG C^GGCCG
3415 CGGCCG
KasI 1403 GGCGCC G^GCGCC
1732 GGCGCC
MfeI 5609 CAATTG C^AATTG
5654 CAATTG
MunI 5609 CAATTG C^AATTG
5654 CAATTG
NarI 1403 GGCGCC GG^CGCC
1732 GGCGCC
NspI 888 GCATGC [GA]CATG^[CT]
5750 ACATGC
SacI 4890 GAGCTC GAGCT^C
5800 GAGCTC
SfoI 1403 GGCGCC GGC^GCC
1732 GGCGCC
StuI 636 AGGCCT AGG^CCT
3994 AGGCCT
TfiI 2678 GATTC G^A[AT]TC
5791 GAATC
XmnI 1657 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3992 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI