################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## Acc65I AclI AgeI Alw44I ApaLI AscI Asp718I AviII BsiEI BsiWI BsmI BspDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraIII EagI EclXI Eco52I EcoRV EcoT22I FseI FspI HpaI KpnI KspI MluI NaeI NgoMIV NheI NotI NruI NsiI NspV PaeR7I PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SacII SalI SfiI SfuI SgrAI SnaBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 1371 GACGTC GACGT^C AccIII 3195 TCCGGA T^CCGGA AflII 4829 CTTAAG C^TTAAG AhdI 4589 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 2162 AGCGCT AGC^GCT Asp700I 2600 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4589 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 2285 CCTAGG C^CTAGG BamHI 5051 GGATCC G^GATCC BbeI 1137 GGCGCC GGCGC^C BbsI 1374 GTCTTC GAAGAC(2/6) BfrI 4829 CTTAAG C^TTAAG BlnI 2285 CCTAGG C^CTAGG BlpI 667 GCTAAGC GC^T[ACGT]AGC Bpu1102I 667 GCTAAGC GC^T[ACGT]AGC BpuAI 1374 GTCTTC GAAGAC(2/6) BseAI 3195 TCCGGA T^CCGGA Bsp1407I 6054 TGTACA T^GTACA BspEI 3195 TCCGGA T^CCGGA BsrBI 2986 CCGCTC CCGCTC(-3/-3) BsrDI 5230 CATTGC GCAATG(2/0) BsrFI 4566 GCCGGT [GA]^CCGG[CT] BsrGI 6054 TGTACA T^GTACA BstEII 3817 GGTAACC G^GT[ACGT]ACC BstPI 3817 GGTAACC G^GT[ACGT]ACC CelII 667 GCTAAGC GC^T[ACGT]AGC Cfr10I 4566 GCCGGT [GA]^CCGG[CT] Eam1105I 4589 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2162 AGCGCT AGC^GCT EcoNI 3433 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 3817 GGTAACC G^GT[ACGT]ACC EcoRI 2604 GAATTC G^AATTC KasI 1137 GGCGCC G^GCGCC MfeI 5530 CAATTG C^AATTG MroI 3195 TCCGGA T^CCGGA MunI 5530 CAATTG C^AATTG NarI 1137 GGCGCC GG^CGCC NdeI 6322 CATATG CA^TATG SapI 722 GCTCTTC GCTCTTC(1/4) SbfI 3823 CCTGCAGG CCTGCA^GG ScaI 1961 AGTACT AGT^ACT SexAI 5376 ACCTGGT A^CC[AT]GGT SfoI 1137 GGCGCC GGC^GCC SpeI 801 ACTAGT A^CTAGT SphI 5735 GCATGC GCATG^C Sse8387I 3823 CCTGCAGG CCTGCA^GG SspBI 6054 TGTACA T^GTACA SspI 420 AATATT AAT^ATT TaqI 1582 TCGA T^CGA TthHB8I 1582 TCGA T^CGA XbaI 5064 TCTAGA T^CTAGA XmnI 2600 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 1274 CGCG CG^CG 5820 CGCG ApaI 297 GGGCCC GGGCC^C 715 GGGCCC AseI 1506 ATTAAT AT^TAAT 1571 ATTAAT AsnI 1506 ATTAAT AT^TAAT 1571 ATTAAT BbvCI 4270 CCTCAGC CCTCAGC(-5/-2) 4405 CCTCAGC BcgI 4259 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4107 GCAGTGGCCTCG BciVI 2370 GGATAC GTATCC(6/5) 2936 GTATCC BclI 116 TGATCA T^GATCA 2709 TGATCA BssSI 2295 CACGAG CACGAG(-5/-1) 2893 CACGAG BstUI 1274 CGCG CG^CG 5820 CGCG DrdI 3522 GACACAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3561 GACTTTGCGGTC FbaI 116 TGATCA T^GATCA 2709 TGATCA HgaI 3795 GACGC GACGC(5/10) 1631 GCGTC HincII 2399 GTCAAC GT[CT]^[GA]AC 5427 GTCAAC HindII 2399 GTCAAC GT[CT]^[GA]AC 5427 GTCAAC MvnI 1274 CGCG CG^CG 5820 CGCG NcoI 1674 CCATGG C^CATGG 6403 CCATGG PacI 1572 TTAATTAA TTAAT^TAA 6335 TTAATTAA PflMI 2760 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4526 CCATAAACTGG PshBI 1506 ATTAAT AT^TAAT 1571 ATTAAT StuI 2762 AGGCCT AGG^CCT 6129 AGGCCT Van91I 2760 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4526 CCATAAACTGG XcmI 370 CCACCTGCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5576 CCACAGGGAGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbrPI BbvI BcnI BfaI BglI BglII BmyI BpmI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PmaCI PmlI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I XhoII XmaCI XmaI