##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AhdI        ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvrII       BclI        
BfrI        BlnI        BsaI        BseAI       BsiWI       
BspEI       BspLU11I    BsrDI       Bst1107I    BstBI       
BstZ17I     CpoI        DrdI        Eam1105I    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HpaI        
KpnI        MfeI        MroI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SbfI        SfuI        
SnaBI       SpeI        Sse8387I    SspI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccI         4996  GTCGAC               GT^[AC][GT]AC
AgeI          820  ACCGGT               A^CCGGT
Aor51HI      5687  AGCGCT               AGC^GCT
AviII        3257  TGCGCA               TGC^GCA
BamHI        6463  GGATCC               G^GATCC
BbrPI        2212  CACGTG               CAC^GTG
BbvCI        3637  GCTGAGG              CCTCAGC(-5/-2)
BciVI        5360  GTATCC               GTATCC(6/5)
BfaI          760  CTAG                 C^TAG
BsmI         5739  GAATGC               GAATGC(1/-1)
Bsp1407I     4685  TGTACA               T^GTACA
BspDI        2008  ATCGAT               AT^CGAT
BspHI        4565  TCATGA               T^CATGA
BsrGI        4685  TGTACA               T^GTACA
ClaI         2008  ATCGAT               AT^CGAT
DraI         6720  TTTAAA               TTT^AAA
Eco47III     5687  AGCGCT               AGC^GCT
EcoNI        5340  CCTGTTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI         3257  TGCGCA               TGC^GCA
MaeI          760  CTAG                 C^TAG
PinAI         820  ACCGGT               A^CCGGT
PmaCI        2212  CACGTG               CAC^GTG
PmlI         2212  CACGTG               CAC^GTG
RcaI         4565  TCATGA               T^CATGA
SacI         3211  GAGCTC               GAGCT^C
SalI         4996  GTCGAC               G^TCGAC
SexAI        2606  ACCAGGT              A^CC[AT]GGT
SfiI          450  GGCCGAGGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        1158  CGCCGGCG             C[GA]^CCGG[CT]G
SspBI        4685  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AatII        1788  GACGTC               GACGT^C
             4993  GACGTC              
AocI         2425  CCTCAGG              CC^T[ACGT]AGG
             3289  CCTGAGG             
Asp700I      5193  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5775  GAATGAGTTC          
BcgI         4148  GCAAAGGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5253  GCAGAGCCATCG        
Bsu36I       2425  CCTCAGG              CC^T[ACGT]AGG
             3289  CCTGAGG             
Eco81I       2425  CCTCAGG              CC^T[ACGT]AGG
             3289  CCTGAGG             
HindIII      5083  AAGCTT               A^AGCTT
             6724  AAGCTT              
MluI          743  ACGCGT               A^CGCGT
             1067  ACGCGT              
PshAI        2770  GACCTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4920  GACACAGGTC          
ScaI         4550  AGTACT               AGT^ACT
             5393  AGTACT              
SphI          713  GCATGC               GCATG^C
             1685  GCATGC              
XmnI         5193  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5775  GAATGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  153 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbeI        BbsI        BbvI        
BcnI        BglI        BglII       BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssHII      BssKI       
BssSI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     KspI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacII       SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI