##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AspEI       AspI        AviII       BbrPI       BseAI       
BsiEI       BsiWI       BspEI       BspHI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      FseI        FspI        KspI        
MluI        MroI        NaeI        NgoMIV      NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I       3074  GGTACC               G^GTACC
AflII        3147  CTTAAG               C^TTAAG
AflIII       5584  ACATGT               A^C[GA][CT]GT
Asp718I      3074  GGTACC               G^GTACC
BalI         1091  TGGCCA               TGG^CCA
BamHI        4230  GGATCC               G^GATCC
BbvCI        5376  CCTCAGC              CCTCAGC(-5/-2)
BcgI         5365  CGATTCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         3147  CTTAAG               C^TTAAG
BsaAI        6100  TACGTG               [CT]AC^GT[GA]
BsaBI        3230  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1386  ACCGGA               [AT]^CCGG[AT]
BspLU11I     5584  ACATGT               A^CATGT
BsrBI        2814  GAGCGG               CCGCTC(-3/-3)
BsrDI          61  GCAATG               GCAATG(2/0)
BsrFI         355  ACCGGC               [GA]^CCGG[CT]
BstAPI       4760  GCATGGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        355  ACCGGC               [GA]^CCGG[CT]
EaeI         1091  TGGCCA               [CT]^GGCC[GA]
EcoRI         240  GAATTC               G^AATTC
EcoRV        1264  GATATC               GAT^ATC
EcoT22I       729  ATGCAT               ATGCA^T
KpnI         3074  GGTACC               GGTAC^C
MaeII        6101  ACGT                 A^CGT
MamI         3230  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1735  CAATTG               C^AATTG
MluNI        1091  TGGCCA               TGG^CCA
MscI         1091  TGGCCA               TGG^CCA
MunI         1735  CAATTG               C^AATTG
NheI         5065  GCTAGC               G^CTAGC
NsiI          729  ATGCAT               ATGCA^T
PaeR7I       4032  CTCGAG               C^TCGAG
XbaI         4316  TCTAGA               T^CTAGA
XhoI         4032  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII          29  CGCG                 CG^CG
              108  CGCG                
AseI         5040  ATTAAT               AT^TAAT
             5950  ATTAAT              
AsnI         5040  ATTAAT               AT^TAAT
             5950  ATTAAT              
AvrII        1665  CCTAGG               C^CTAGG
             4497  CCTAGG              
BbeI           95  GGCGCC               GGCGC^C
             3748  GGCGCC              
BclI         4251  TGATCA               T^GATCA
             4969  TGATCA              
BlnI         1665  CCTAGG               C^CTAGG
             4497  CCTAGG              
BsmBI        6410  CGTCTC               CGTCTC(1/5)
             3081  GAGACG              
Bsp1407I     1702  TGTACA               T^GTACA
             3115  TGTACA              
BspDI        3267  ATCGAT               AT^CGAT
             3843  ATCGAT              
BsrGI        1702  TGTACA               T^GTACA
             3115  TGTACA              
BstUI          29  CGCG                 CG^CG
              108  CGCG                
ClaI         3267  ATCGAT               AT^CGAT
             3843  ATCGAT              
FbaI         4251  TGATCA               T^GATCA
             4969  TGATCA              
HaeII          95  GGCGCC               [GA]GCGC^[CT]
             3748  GGCGCC              
HgaI         3083  GACGC                GACGC(5/10)
             3803  GCGTC               
HpaI         2147  GTTAAC               GTT^AAC
             4922  GTTAAC              
KasI           95  GGCGCC               G^GCGCC
             3748  GGCGCC              
MvnI           29  CGCG                 CG^CG
              108  CGCG                
NarI           95  GGCGCC               GG^CGCC
             3748  GGCGCC              
PflMI        3850  CCAGACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4057  CCATCTGTTGG         
PshBI        5040  ATTAAT               AT^TAAT
             5950  ATTAAT              
SacI          606  GAGCTC               GAGCT^C
             4023  GAGCTC              
SapI          947  GAAGAGC              GCTCTTC(1/4)
             4188  GCTCTTC             
SexAI        5636  ACCAGGT              A^CC[AT]GGT
             6313  ACCAGGT             
SfoI           95  GGCGCC               GGC^GCC
             3748  GGCGCC              
SspBI        1702  TGTACA               T^GTACA
             3115  TGTACA              
SspI          648  AATATT               AAT^ATT
             6559  AATATT              
Van91I       3850  CCAGACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4057  CCATCTGTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        ApoI        
Asp700I     AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvI        BciVI       BcnI        
BfaI        BglI        BglII       BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstXI       BstYI       
Bsu36I      Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EarI        Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeI        NdeII       NlaIII      
NlaIV       NspI        PleI        PpuMI       PshAI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
SphI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII       XmaCI       XmaI        
XmnI