################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AccI AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AspEI AspI AviII BbrPI BseAI BsiEI BsiWI BspEI BspHI BssHII BssSI Bst1107I BstBI BstZ17I CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI KspI MluI MroI NaeI NgoMIV NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI Tth111I ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 3074 GGTACC G^GTACC AflII 3147 CTTAAG C^TTAAG AflIII 5584 ACATGT A^C[GA][CT]GT Asp718I 3074 GGTACC G^GTACC BalI 1091 TGGCCA TGG^CCA BamHI 4230 GGATCC G^GATCC BbvCI 5376 CCTCAGC CCTCAGC(-5/-2) BcgI 5365 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 3147 CTTAAG C^TTAAG BsaAI 6100 TACGTG [CT]AC^GT[GA] BsaBI 3230 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1386 ACCGGA [AT]^CCGG[AT] BspLU11I 5584 ACATGT A^CATGT BsrBI 2814 GAGCGG CCGCTC(-3/-3) BsrDI 61 GCAATG GCAATG(2/0) BsrFI 355 ACCGGC [GA]^CCGG[CT] BstAPI 4760 GCATGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 355 ACCGGC [GA]^CCGG[CT] EaeI 1091 TGGCCA [CT]^GGCC[GA] EcoRI 240 GAATTC G^AATTC EcoRV 1264 GATATC GAT^ATC EcoT22I 729 ATGCAT ATGCA^T KpnI 3074 GGTACC GGTAC^C MaeII 6101 ACGT A^CGT MamI 3230 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1735 CAATTG C^AATTG MluNI 1091 TGGCCA TGG^CCA MscI 1091 TGGCCA TGG^CCA MunI 1735 CAATTG C^AATTG NheI 5065 GCTAGC G^CTAGC NsiI 729 ATGCAT ATGCA^T PaeR7I 4032 CTCGAG C^TCGAG XbaI 4316 TCTAGA T^CTAGA XhoI 4032 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 29 CGCG CG^CG 108 CGCG AseI 5040 ATTAAT AT^TAAT 5950 ATTAAT AsnI 5040 ATTAAT AT^TAAT 5950 ATTAAT AvrII 1665 CCTAGG C^CTAGG 4497 CCTAGG BbeI 95 GGCGCC GGCGC^C 3748 GGCGCC BclI 4251 TGATCA T^GATCA 4969 TGATCA BlnI 1665 CCTAGG C^CTAGG 4497 CCTAGG BsmBI 6410 CGTCTC CGTCTC(1/5) 3081 GAGACG Bsp1407I 1702 TGTACA T^GTACA 3115 TGTACA BspDI 3267 ATCGAT AT^CGAT 3843 ATCGAT BsrGI 1702 TGTACA T^GTACA 3115 TGTACA BstUI 29 CGCG CG^CG 108 CGCG ClaI 3267 ATCGAT AT^CGAT 3843 ATCGAT FbaI 4251 TGATCA T^GATCA 4969 TGATCA HaeII 95 GGCGCC [GA]GCGC^[CT] 3748 GGCGCC HgaI 3083 GACGC GACGC(5/10) 3803 GCGTC HpaI 2147 GTTAAC GTT^AAC 4922 GTTAAC KasI 95 GGCGCC G^GCGCC 3748 GGCGCC MvnI 29 CGCG CG^CG 108 CGCG NarI 95 GGCGCC GG^CGCC 3748 GGCGCC PflMI 3850 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4057 CCATCTGTTGG PshBI 5040 ATTAAT AT^TAAT 5950 ATTAAT SacI 606 GAGCTC GAGCT^C 4023 GAGCTC SapI 947 GAAGAGC GCTCTTC(1/4) 4188 GCTCTTC SexAI 5636 ACCAGGT A^CC[AT]GGT 6313 ACCAGGT SfoI 95 GGCGCC GGC^GCC 3748 GGCGCC SspBI 1702 TGTACA T^GTACA 3115 TGTACA SspI 648 AATATT AAT^ATT 6559 AATATT Van91I 3850 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4057 CCATCTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BciVI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI XmnI