##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AseI AsnI
Asp718I AspEI BciVI BclI BfrI
BglII BsaBI BsiEI BsiWI BsmI
BspDI BspLU11I BstEII BstPI ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MunI NaeI NgoMIV NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 566 TCCGGA T^CCGGA
AcsI 2131 GAATTC [GA]^AATT[CT]
AflIII 1831 ACACGT A^C[GA][CT]GT
ApoI 2131 GAATTC [GA]^AATT[CT]
AscI 1451 GGCGCGCC GG^CGCGCC
AspI 528 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 563 GGATCC G^GATCC
BglI 3700 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 566 TCCGGA [AT]^CCGG[AT]
BseAI 566 TCCGGA T^CCGGA
BsmBI 210 GAGACG CGTCTC(1/5)
Bsp1407I 851 TGTACA T^GTACA
BspEI 566 TCCGGA T^CCGGA
BsrGI 851 TGTACA T^GTACA
DraIII 2242 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 2131 GAATTC G^AATTC
HindIII 2060 AAGCTT A^AGCTT
MroI 566 TCCGGA T^CCGGA
NcoI 2384 CCATGG C^CATGG
NdeI 2445 CATATG CA^TATG
NheI 4025 GCTAGC G^CTAGC
PflFI 528 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 233 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1522 GAAGAGC GCTCTTC(1/4)
ScaI 2425 AGTACT AGT^ACT
SexAI 2566 ACCTGGT A^CC[AT]GGT
SmaI 164 CCCGGG CCC^GGG
SspBI 851 TGTACA T^GTACA
Tth111I 528 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 241 TCTAGA T^CTAGA
XcmI 2384 CCATGGCCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 164 CCCGGG C^CCGGG
XmaI 164 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 311 GTATAC GT^[AC][GT]AC
1686 GTATAC
AocI 3877 CCTGAGG CC^T[ACGT]AGG
4072 CCTCAGG
Aor51HI 256 AGCGCT AGC^GCT
2576 AGCGCT
Asp700I 759 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2058 GAAAGCTTTC
AvaI 164 CCCGGG C^[CT]CG[GA]G
2390 CCCGAG
AvrII 3393 CCTAGG C^CTAGG
3622 CCTAGG
BbeI 1796 GGCGCC GGCGC^C
3982 GGCGCC
BbrPI 1068 CACGTG CAC^GTG
2874 CACGTG
BcgI 3830 CGAGCAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1661 GCAAATGTATCG
BlnI 3393 CCTAGG C^CTAGG
3622 CCTAGG
BsaI 3217 GAGACC GGTCTC(1/5)
1577 GGTCTC
BsoBI 164 CCCGGG C^[CT]CG[GA]G
2390 CCCGAG
BspHI 392 TCATGA T^CATGA
933 TCATGA
BsrDI 103 CATTGC GCAATG(2/0)
3186 CATTGC
BsrFI 635 GCCGGT [GA]^CCGG[CT]
2648 ACCGGC
BssSI 802 CACGAG CACGAG(-5/-1)
187 CTCGTG
Bst1107I 311 GTATAC GTA^TAC
1686 GTATAC
BstBI 692 TTCGAA TT^CGAA
766 TTCGAA
BstZ17I 311 GTATAC GTA^TAC
1686 GTATAC
Bsu36I 3877 CCTGAGG CC^T[ACGT]AGG
4072 CCTCAGG
Cfr10I 635 GCCGGT [GA]^CCGG[CT]
2648 ACCGGC
Eco47III 256 AGCGCT AGC^GCT
2576 AGCGCT
Eco81I 3877 CCTGAGG CC^T[ACGT]AGG
4072 CCTCAGG
KasI 1796 GGCGCC G^GCGCC
3982 GGCGCC
MslI 1357 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1652 CACTGCTGTG
NarI 1796 GGCGCC GG^CGCC
3982 GGCGCC
NspV 692 TTCGAA TT^CGAA
766 TTCGAA
PleI 1784 GACTC GAGTC(4/5)
2753 GACTC
PmaCI 1068 CACGTG CAC^GTG
2874 CACGTG
PmlI 1068 CACGTG CAC^GTG
2874 CACGTG
RcaI 392 TCATGA T^CATGA
933 TCATGA
SfoI 1796 GGCGCC GGC^GCC
3982 GGCGCC
SfuI 692 TTCGAA TT^CGAA
766 TTCGAA
SphI 73 GCATGC GCATG^C
1623 GCATGC
XmnI 759 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2058 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaII
AviII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII