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# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AspI AviII BamHI BbrPI BcgI
BfrI BsiEI BsiWI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I EagI
EclXI Eco52I EcoO65I EcoRV EcoT22I
FseI FspI HindIII HpaI KspI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 3122 GTCTAC GT^[AC][GT]AC
Alw44I 1621 GTGCAC G^TGCAC
Aor51HI 5983 AGCGCT AGC^GCT
ApaLI 1621 GTGCAC G^TGCAC
Asp700I 3618 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 5515 GCTGAGG CCTCAGC(-5/-2)
BglII 1464 AGATCT A^GATCT
BsaBI 1750 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 897 TGTACA T^GTACA
BspDI 2045 ATCGAT AT^CGAT
BspLU11I 423 ACATGT A^CATGT
BsrGI 897 TGTACA T^GTACA
ClaI 2045 ATCGAT AT^CGAT
CpoI 2184 CGGTCCG CG^G[AT]CCG
DrdI 4376 GACAGGCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5983 AGCGCT AGC^GCT
HincII 1412 GTCAAC GT[CT]^[GA]AC
HindII 1412 GTCAAC GT[CT]^[GA]AC
MamI 1750 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3105 GCCGGC GCC^GGC
NgoMIV 3105 GCCGGC G^CCGGC
PaeR7I 4237 CTCGAG C^TCGAG
RsrII 2184 CGGTCCG CG^G[AT]CCG
ScaI 5061 AGTACT AGT^ACT
SspBI 897 TGTACA T^GTACA
SspI 728 AATATT AAT^ATT
XbaI 661 TCTAGA T^CTAGA
XhoI 4237 CTCGAG C^TCGAG
XmnI 3618 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 477 TCCGGA T^CCGGA
1881 TCCGGA
AflIII 423 ACATGT A^C[GA][CT]GT
6036 ACGTGT
AhdI 341 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3340 GACAGGGAGTC
AseI 725 ATTAAT AT^TAAT
4131 ATTAAT
AsnI 725 ATTAAT AT^TAAT
4131 ATTAAT
AspEI 341 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3340 GACAGGGAGTC
BbeI 2140 GGCGCC GGCGC^C
4789 GGCGCC
BbsI 1433 GAAGAC GAAGAC(2/6)
2084 GTCTTC
BciVI 2774 GGATAC GTATCC(6/5)
3298 GTATCC
BclI 276 TGATCA T^GATCA
3378 TGATCA
BglI 2450 GCCTACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4537 GCCCTAGAGGC
BpuAI 1433 GAAGAC GAAGAC(2/6)
2084 GTCTTC
BsaAI 2453 TACGTG [CT]AC^GT[GA]
6035 TACGTG
BsaWI 477 TCCGGA [AT]^CCGG[AT]
1881 TCCGGA
BseAI 477 TCCGGA T^CCGGA
1881 TCCGGA
BspEI 477 TCCGGA T^CCGGA
1881 TCCGGA
BstXI 11 CCAGAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5156 CCAGCCCTGTGG
DraIII 1615 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1994 CACTGCGTG
Eam1105I 341 GACAAACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3340 GACAGGGAGTC
EcoRI 2501 GAATTC G^AATTC
5112 GAATTC
FbaI 276 TGATCA T^GATCA
3378 TGATCA
KasI 2140 GGCGCC G^GCGCC
4789 GGCGCC
MroI 477 TCCGGA T^CCGGA
1881 TCCGGA
NarI 2140 GGCGCC GG^CGCC
4789 GGCGCC
PshBI 725 ATTAAT AT^TAAT
4131 ATTAAT
SfoI 2140 GGCGCC GGC^GCC
4789 GGCGCC
SmaI 855 CCCGGG CCC^GGG
3323 CCCGGG
XmaCI 855 CCCGGG C^CCGGG
3323 CCCGGG
XmaI 855 CCCGGG C^CCGGG
3323 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI Asp718I AspHI AvaI
AvaII AvrII BalI BanI BanII
BbvI BcnI BfaI BlnI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII