##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AccI AccIII AclI AscI AseI
AsnI AspI BsaBI BseAI BsiWI
BspDI BspEI BspHI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EcoRV FseI HpaI MamI MfeI
MluI MroI MunI NotI NruI
NspV PacI PflFI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI SfuI SgrAI SpeI
Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1067 GACGTC GACGT^C
AgeI 552 ACCGGT A^CCGGT
AhdI 1424 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2277 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1424 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4375 TGCGCA TGC^GCA
BbrPI 224 CACGTG CAC^GTG
BclI 2502 TGATCA T^GATCA
BglII 5490 AGATCT A^GATCT
BsmI 546 GCATTC GAATGC(1/-1)
Bsp1407I 606 TGTACA T^GTACA
BspLU11I 246 ACATGT A^CATGT
BsrDI 4379 CATTGC GCAATG(2/0)
BsrGI 606 TGTACA T^GTACA
BstEII 484 GGTGACC G^GT[ACGT]ACC
BstPI 484 GGTGACC G^GT[ACGT]ACC
Eam1105I 1424 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 484 GGTGACC G^GT[ACGT]ACC
EcoT22I 5932 ATGCAT ATGCA^T
FbaI 2502 TGATCA T^GATCA
FspI 4375 TGCGCA TGC^GCA
HincII 3771 GTCAAC GT[CT]^[GA]AC
HindII 3771 GTCAAC GT[CT]^[GA]AC
HindIII 5583 AAGCTT A^AGCTT
NaeI 4073 GCCGGC GCC^GGC
NdeI 5929 CATATG CA^TATG
NgoMIV 4073 GCCGGC G^CCGGC
NsiI 5932 ATGCAT ATGCA^T
PinAI 552 ACCGGT A^CCGGT
PmaCI 224 CACGTG CAC^GTG
PmlI 224 CACGTG CAC^GTG
SapI 4308 GAAGAGC GCTCTTC(1/4)
SnaBI 4200 TACGTA TAC^GTA
SspBI 606 TGTACA T^GTACA
StuI 4901 AGGCCT AGG^CCT
XmnI 2277 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 2808 GAATTC [GA]^AATT[CT]
4878 GAATTC
AflIII 246 ACATGT A^C[GA][CT]GT
3811 ACACGT
Aor51HI 2279 AGCGCT AGC^GCT
4150 AGCGCT
ApoI 2808 GAATTC [GA]^AATT[CT]
4878 GAATTC
BbeI 3698 GGCGCC GGCGC^C
4415 GGCGCC
BsmBI 2526 CGTCTC CGTCTC(1/5)
2604 GAGACG
BssSI 4548 CACGAG CACGAG(-5/-1)
4644 CTCGTG
DraIII 188 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1270 CACTGGGTG
EagI 125 CGGCCG C^GGCCG
4302 CGGCCG
EclXI 125 CGGCCG C^GGCCG
4302 CGGCCG
Eco47III 2279 AGCGCT AGC^GCT
4150 AGCGCT
Eco52I 125 CGGCCG C^GGCCG
4302 CGGCCG
EcoRI 2808 GAATTC G^AATTC
4878 GAATTC
KasI 3698 GGCGCC G^GCGCC
4415 GGCGCC
KspI 207 CCGCGG CCGC^GG
5229 CCGCGG
NarI 3698 GGCGCC GG^CGCC
4415 GGCGCC
NheI 202 GCTAGC G^CTAGC
3243 GCTAGC
PaeR7I 5390 CTCGAG C^TCGAG
5860 CTCGAG
SacI 1085 GAGCTC GAGCT^C
1539 GAGCTC
SacII 207 CCGCGG CCGC^GG
5229 CCGCGG
ScaI 1630 AGTACT AGT^ACT
4757 AGTACT
SexAI 2785 ACCAGGT A^CC[AT]GGT
3459 ACCTGGT
SfoI 3698 GGCGCC GGC^GCC
4415 GGCGCC
SmaI 2675 CCCGGG CCC^GGG
3184 CCCGGG
SspI 2174 AATATT AAT^ATT
2953 AATATT
XhoI 5390 CTCGAG C^TCGAG
5860 CTCGAG
XmaCI 2675 CCCGGG C^CCGGG
3184 CCCGGG
XmaI 2675 CCCGGG C^CCGGG
3184 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI Asp718I AspHI
AvaI AvaII AvrII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcgI BciVI BcnI BfaI BfrI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII