##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI BbrPI BfrI
BsaBI BsiWI BspDI BsrBI BsrFI
BssHII BstEII BstPI Cfr10I ClaI
CpoI DrdI Eco47III EcoNI EcoO65I
FseI KspI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SgrAI SnaBI Sse8387I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 2090 TCCGGA T^CCGGA
AflIII 3739 ACATGT A^C[GA][CT]GT
AlwNI 4398 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2267 GGGCCC GGGCC^C
AseI 3169 ATTAAT AT^TAAT
AsnI 3169 ATTAAT AT^TAAT
Asp700I 3521 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 963 TGCGCA TGC^GCA
AvrII 3456 CCTAGG C^CTAGG
BclI 2428 TGATCA T^GATCA
BglII 4870 AGATCT A^GATCT
BlnI 3456 CCTAGG C^CTAGG
BseAI 2090 TCCGGA T^CCGGA
BsiEI 5295 CGGCCG CG[GA][CT]^CG
Bsp1407I 682 TGTACA T^GTACA
BspEI 2090 TCCGGA T^CCGGA
BspLU11I 3739 ACATGT A^CATGT
BspMI 2030 ACCTGC ACCTGC(4/8)
BsrGI 682 TGTACA T^GTACA
BssSI 6076 CACGAG CACGAG(-5/-1)
Bst1107I 4855 GTATAC GTA^TAC
BstBI 173 TTCGAA TT^CGAA
BstZ17I 4855 GTATAC GTA^TAC
EagI 5295 CGGCCG C^GGCCG
EclXI 5295 CGGCCG C^GGCCG
Eco52I 5295 CGGCCG C^GGCCG
EcoRI 3853 GAATTC G^AATTC
FbaI 2428 TGATCA T^GATCA
FspI 963 TGCGCA TGC^GCA
HindIII 866 AAGCTT A^AGCTT
HpaI 498 GTTAAC GTT^AAC
MroI 2090 TCCGGA T^CCGGA
NspV 173 TTCGAA TT^CGAA
PaeR7I 4839 CTCGAG C^TCGAG
PflMI 1008 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3169 ATTAAT AT^TAAT
SexAI 320 ACCTGGT A^CC[AT]GGT
SfuI 173 TTCGAA TT^CGAA
SphI 2776 GCATGC GCATG^C
SspBI 682 TGTACA T^GTACA
SspI 3242 AATATT AAT^ATT
Van91I 1008 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3766 TCTAGA T^CTAGA
XhoI 4839 CTCGAG C^TCGAG
XmnI 3521 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AccII 5063 CGCG CG^CG
6250 CGCG
AcyI 1860 GGCGCC G[GA]^CG[CT]C
6166 GGCGCC
AhdI 600 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
727 GACCAAAAGTC
AspEI 600 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
727 GACCAAAAGTC
AspI 319 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6285 GACTCCGTC
BalI 467 TGGCCA TGG^CCA
3389 TGGCCA
BamHI 2685 GGATCC G^GATCC
5866 GGATCC
BbeI 1860 GGCGCC GGCGC^C
6166 GGCGCC
BbvCI 1425 GCTGAGG CCTCAGC(-5/-2)
6194 GCTGAGG
BcgI 1560 GCACCTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2156 GCACGGTCTTCG
BlpI 2283 GCTGAGC GC^T[ACGT]AGC
2636 GCTAAGC
Bpu1102I 2283 GCTGAGC GC^T[ACGT]AGC
2636 GCTAAGC
BsaAI 634 TACGTG [CT]AC^GT[GA]
2245 TACGTG
BsaHI 1860 GGCGCC G[GA]^CG[CT]C
6166 GGCGCC
BsaWI 884 ACCGGA [AT]^CCGG[AT]
2090 TCCGGA
BsmBI 6123 CGTCTC CGTCTC(1/5)
6290 CGTCTC
BstAPI 1077 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1351 GCAAATGTTGC
BstUI 5063 CGCG CG^CG
6250 CGCG
CelII 2283 GCTGAGC GC^T[ACGT]AGC
2636 GCTAAGC
DraII 1612 AGGCCCC [GA]G^G[ACGT]CC[CT]
2267 GGGCCCC
DraIII 2625 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
5033 CACTCTGTG
Eam1105I 600 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
727 GACCAAAAGTC
EcoO109I 1612 AGGCCCC [GA]G^G[ACGT]CC[CT]
2267 GGGCCCC
EcoRV 1132 GATATC GAT^ATC
4373 GATATC
EcoT22I 1002 ATGCAT ATGCA^T
2445 ATGCAT
HgaI 1620 GACGC GACGC(5/10)
4481 GCGTC
Hin1I 1860 GGCGCC G[GA]^CG[CT]C
6166 GGCGCC
KasI 1860 GGCGCC G^GCGCC
6166 GGCGCC
MluNI 467 TGGCCA TGG^CCA
3389 TGGCCA
MscI 467 TGGCCA TGG^CCA
3389 TGGCCA
MvnI 5063 CGCG CG^CG
6250 CGCG
NarI 1860 GGCGCC GG^CGCC
6166 GGCGCC
NsiI 1002 ATGCAT ATGCA^T
2445 ATGCAT
PflFI 319 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6285 GACTCCGTC
PstI 1602 CTGCAG CTGCA^G
5140 CTGCAG
SacI 2404 GAGCTC GAGCT^C
5351 GAGCTC
SfoI 1860 GGCGCC GGC^GCC
6166 GGCGCC
SmaI 5093 CCCGGG CCC^GGG
6218 CCCGGG
SpeI 1165 ACTAGT A^CTAGT
4859 ACTAGT
StuI 1429 AGGCCT AGG^CCT
4910 AGGCCT
SwaI 15 ATTTAAAT ATTT^AAAT
4303 ATTTAAAT
Tth111I 319 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6285 GACTCCGTC
XcmI 3803 CCAGGAGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5602 CCAGCTTCCCTGTGG
XmaCI 5093 CCCGGG C^CCGGG
6218 CCCGGG
XmaI 5093 CCCGGG C^CCGGG
6218 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AluI AlwI AocI ApoI Asp718I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BciVI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EarI Eco81I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII