################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII Acc65I AflII AscI Asp718I AspI AvrII BamHI BcgI BfrI BglII BlnI BsaBI BsiWI Bsp1407I BspDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FseI HpaI KpnI MamI MluI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI RsrII SalI SbfI ScaI SfiI SfuI SmaI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 1466 GTCTAC GT^[AC][GT]AC AclI 1845 AACGTT AA^CGTT AflIII 1006 ACATGT A^C[GA][CT]GT AgeI 4448 ACCGGT A^CCGGT AhdI 1005 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 5263 ATTAAT AT^TAAT AsnI 5263 ATTAAT AT^TAAT AspEI 1005 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3793 TGCGCA TGC^GCA BbeI 3770 GGCGCC GGCGC^C BbsI 1013 GTCTTC GAAGAC(2/6) BciVI 1210 GTATCC GTATCC(6/5) BpuAI 1013 GTCTTC GAAGAC(2/6) BsmBI 3137 GAGACG CGTCTC(1/5) BspLU11I 1006 ACATGT A^CATGT BssSI 2954 CTCGTG CACGAG(-5/-1) BstAPI 4808 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 2303 GACCCAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1005 GACATGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 5099 GAATTC G^AATTC FspI 3793 TGCGCA TGC^GCA HgaI 204 GCGTC GACGC(5/10) HincII 3582 GTCAAC GT[CT]^[GA]AC HindII 3582 GTCAAC GT[CT]^[GA]AC HindIII 1201 AAGCTT A^AGCTT KasI 3770 GGCGCC G^GCGCC KspI 1114 CCGCGG CCGC^GG NarI 3770 GGCGCC GG^CGCC PinAI 4448 ACCGGT A^CCGGT PshAI 1825 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 5263 ATTAAT AT^TAAT Psp1406I 1845 AACGTT AA^CGTT PvuI 1906 CGATCG CGAT^CG SacII 1114 CCGCGG CCGC^GG SapI 1790 GCTCTTC GCTCTTC(1/4) SexAI 2349 ACCTGGT A^CC[AT]GGT SfoI 3770 GGCGCC GGC^GCC SgrAI 4447 CACCGGTG C[GA]^CCGG[CT]G SnaBI 3809 TACGTA TAC^GTA ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccIII 3537 TCCGGA T^CCGGA 3826 TCCGGA AcyI 203 GGCGTC G[GA]^CG[CT]C 3770 GGCGCC Aor51HI 714 AGCGCT AGC^GCT 2130 AGCGCT Asp700I 2014 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5261 GAATTAATTC BanI 1868 GGCACC G^G[CT][GA]CC 3770 GGCGCC BbrPI 818 CACGTG CAC^GTG 1124 CACGTG BfaI 698 CTAG C^TAG 5073 CTAG BlpI 2074 GCTGAGC GC^T[ACGT]AGC 3521 GCTGAGC Bpu1102I 2074 GCTGAGC GC^T[ACGT]AGC 3521 GCTGAGC BsaHI 203 GGCGTC G[GA]^CG[CT]C 3770 GGCGCC BseAI 3537 TCCGGA T^CCGGA 3826 TCCGGA BsiEI 538 CGACCG CG[GA][CT]^CG 1906 CGATCG BsmI 1747 GAATGC GAATGC(1/-1) 4698 GCATTC BspEI 3537 TCCGGA T^CCGGA 3826 TCCGGA BspHI 861 TCATGA T^CATGA 1642 TCATGA BstYI 1195 GGATCT [GA]^GATC[CT] 5075 AGATCC CelII 2074 GCTGAGC GC^T[ACGT]AGC 3521 GCTGAGC DraIII 1124 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG 4436 CACCCAGTG EarI 1791 CTCTTC CTCTTC(1/4) 5107 CTCTTC Eco47III 714 AGCGCT AGC^GCT 2130 AGCGCT EcoNI 2980 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3127 CCTCTGGGAGG Hin1I 203 GGCGTC G[GA]^CG[CT]C 3770 GGCGCC Ksp632I 1791 CTCTTC CTCTTC(1/4) 5107 CTCTTC MaeI 698 CTAG C^TAG 5073 CTAG MfeI 3405 CAATTG C^AATTG 4457 CAATTG MflI 1195 GGATCT [GA]^GATC[CT] 5075 AGATCC MroI 3537 TCCGGA T^CCGGA 3826 TCCGGA MseI 5193 TTAA T^TAA 5264 TTAA MunI 3405 CAATTG C^AATTG 4457 CAATTG NaeI 639 GCCGGC GCC^GGC 1142 GCCGGC NgoMIV 639 GCCGGC G^CCGGC 1142 GCCGGC PmaCI 818 CACGTG CAC^GTG 1124 CACGTG PmlI 818 CACGTG CAC^GTG 1124 CACGTG RcaI 861 TCATGA T^CATGA 1642 TCATGA SacI 1982 GAGCTC GAGCT^C 2249 GAGCTC SphI 594 GCATGC GCATG^C 4326 GCATGC Tru9I 5193 TTAA T^TAA 5264 TTAA XhoII 1195 GGATCT [GA]^GATC[CT] 5075 AGATCC XmnI 2014 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5261 GAATTAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AciI AcsI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AspHI AvaI AvaII BalI BanII BbvCI BbvI BclI BcnI BglI BmyI BpmI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI