##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

AatII       Acc65I      AflII       AscI        Asp718I     
AspI        AvrII       BamHI       BcgI        BfrI        
BglII       BlnI        BsaBI       BsiWI       Bsp1407I    
BspDI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        EagI        EclXI       Eco52I      EcoO65I     
EcoRV       EcoT22I     FseI        HpaI        KpnI        
MamI        MluI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PmeI        RsrII       SalI        SbfI        
ScaI        SfiI        SfuI        SmaI        SpeI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI         1466  GTCTAC               GT^[AC][GT]AC
AclI         1845  AACGTT               AA^CGTT
AflIII       1006  ACATGT               A^C[GA][CT]GT
AgeI         4448  ACCGGT               A^CCGGT
AhdI         1005  GACATGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI         5263  ATTAAT               AT^TAAT
AsnI         5263  ATTAAT               AT^TAAT
AspEI        1005  GACATGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3793  TGCGCA               TGC^GCA
BbeI         3770  GGCGCC               GGCGC^C
BbsI         1013  GTCTTC               GAAGAC(2/6)
BciVI        1210  GTATCC               GTATCC(6/5)
BpuAI        1013  GTCTTC               GAAGAC(2/6)
BsmBI        3137  GAGACG               CGTCTC(1/5)
BspLU11I     1006  ACATGT               A^CATGT
BssSI        2954  CTCGTG               CACGAG(-5/-1)
BstAPI       4808  GCAACCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI         2303  GACCCAAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1005  GACATGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        5099  GAATTC               G^AATTC
FspI         3793  TGCGCA               TGC^GCA
HgaI          204  GCGTC                GACGC(5/10)
HincII       3582  GTCAAC               GT[CT]^[GA]AC
HindII       3582  GTCAAC               GT[CT]^[GA]AC
HindIII      1201  AAGCTT               A^AGCTT
KasI         3770  GGCGCC               G^GCGCC
KspI         1114  CCGCGG               CCGC^GG
NarI         3770  GGCGCC               GG^CGCC
PinAI        4448  ACCGGT               A^CCGGT
PshAI        1825  GACTCATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        5263  ATTAAT               AT^TAAT
Psp1406I     1845  AACGTT               AA^CGTT
PvuI         1906  CGATCG               CGAT^CG
SacII        1114  CCGCGG               CCGC^GG
SapI         1790  GCTCTTC              GCTCTTC(1/4)
SexAI        2349  ACCTGGT              A^CC[AT]GGT
SfoI         3770  GGCGCC               GGC^GCC
SgrAI        4447  CACCGGTG             C[GA]^CCGG[CT]G
SnaBI        3809  TACGTA               TAC^GTA


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccIII       3537  TCCGGA               T^CCGGA
             3826  TCCGGA              
AcyI          203  GGCGTC               G[GA]^CG[CT]C
             3770  GGCGCC              
Aor51HI       714  AGCGCT               AGC^GCT
             2130  AGCGCT              
Asp700I      2014  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5261  GAATTAATTC          
BanI         1868  GGCACC               G^G[CT][GA]CC
             3770  GGCGCC              
BbrPI         818  CACGTG               CAC^GTG
             1124  CACGTG              
BfaI          698  CTAG                 C^TAG
             5073  CTAG                
BlpI         2074  GCTGAGC              GC^T[ACGT]AGC
             3521  GCTGAGC             
Bpu1102I     2074  GCTGAGC              GC^T[ACGT]AGC
             3521  GCTGAGC             
BsaHI         203  GGCGTC               G[GA]^CG[CT]C
             3770  GGCGCC              
BseAI        3537  TCCGGA               T^CCGGA
             3826  TCCGGA              
BsiEI         538  CGACCG               CG[GA][CT]^CG
             1906  CGATCG              
BsmI         1747  GAATGC               GAATGC(1/-1)
             4698  GCATTC              
BspEI        3537  TCCGGA               T^CCGGA
             3826  TCCGGA              
BspHI         861  TCATGA               T^CATGA
             1642  TCATGA              
BstYI        1195  GGATCT               [GA]^GATC[CT]
             5075  AGATCC              
CelII        2074  GCTGAGC              GC^T[ACGT]AGC
             3521  GCTGAGC             
DraIII       1124  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4436  CACCCAGTG           
EarI         1791  CTCTTC               CTCTTC(1/4)
             5107  CTCTTC              
Eco47III      714  AGCGCT               AGC^GCT
             2130  AGCGCT              
EcoNI        2980  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3127  CCTCTGGGAGG         
Hin1I         203  GGCGTC               G[GA]^CG[CT]C
             3770  GGCGCC              
Ksp632I      1791  CTCTTC               CTCTTC(1/4)
             5107  CTCTTC              
MaeI          698  CTAG                 C^TAG
             5073  CTAG                
MfeI         3405  CAATTG               C^AATTG
             4457  CAATTG              
MflI         1195  GGATCT               [GA]^GATC[CT]
             5075  AGATCC              
MroI         3537  TCCGGA               T^CCGGA
             3826  TCCGGA              
MseI         5193  TTAA                 T^TAA
             5264  TTAA                
MunI         3405  CAATTG               C^AATTG
             4457  CAATTG              
NaeI          639  GCCGGC               GCC^GGC
             1142  GCCGGC              
NgoMIV        639  GCCGGC               G^CCGGC
             1142  GCCGGC              
PmaCI         818  CACGTG               CAC^GTG
             1124  CACGTG              
PmlI          818  CACGTG               CAC^GTG
             1124  CACGTG              
RcaI          861  TCATGA               T^CATGA
             1642  TCATGA              
SacI         1982  GAGCTC               GAGCT^C
             2249  GAGCTC              
SphI          594  GCATGC               GCATG^C
             4326  GCATGC              
Tru9I        5193  TTAA                 T^TAA
             5264  TTAA                
XhoII        1195  GGATCT               [GA]^GATC[CT]
             5075  AGATCC              
XmnI         2014  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             5261  GAATTAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
Alw44I      AlwI        AlwNI       AocI        ApaI        
ApaLI       ApoI        AspHI       AvaI        AvaII       
BalI        BanII       BbvCI       BbvI        BclI        
BcnI        BglI        BmyI        BpmI        BsaAI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI