##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AvrII
BamHI BbeI BcgI BciVI BlnI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BstBI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I FseI HaeII HgaI HpaI
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PinAI PmeI
PshAI PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SphI
Sse8387I StuI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2798 GACGTC GACGT^C
AccII 4343 CGCG CG^CG
AclI 3574 AACGTT AA^CGTT
AcyI 2798 GACGTC G[GA]^CG[CT]C
Alw44I 950 GTGCAC G^TGCAC
ApaLI 950 GTGCAC G^TGCAC
AviII 4429 TGCGCA TGC^GCA
BanI 1149 GGTGCC G^G[CT][GA]CC
BcnI 4488 CCGGG CC^[GC]GG
BglI 3341 GCCACAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1705 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1705 GCTAAGC GC^T[ACGT]AGC
BsaBI 2152 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2798 GACGTC G[GA]^CG[CT]C
BseRI 6329 GAGGAG GAGGAG(10/8)
BspMI 1987 ACCTGC ACCTGC(4/8)
BssSI 4725 CACGAG CACGAG(-5/-1)
Bst1107I 6511 GTATAC GTA^TAC
BstEII 414 GGTCACC G^GT[ACGT]ACC
BstPI 414 GGTCACC G^GT[ACGT]ACC
BstUI 4343 CGCG CG^CG
BstZ17I 6511 GTATAC GTA^TAC
CelII 1705 GCTAAGC GC^T[ACGT]AGC
DsaI 3492 CCATGG C^C[GA][CT]GG
EcoNI 1131 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 414 GGTCACC G^GT[ACGT]ACC
EcoT22I 3998 ATGCAT ATGCA^T
FspI 4429 TGCGCA TGC^GCA
Hin1I 2798 GACGTC G[GA]^CG[CT]C
MamI 2152 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 2252 CAGCTG C[AC]G^C[GT]G
MvnI 4343 CGCG CG^CG
NciI 4488 CCGGG CC^[GC]GG
NcoI 3492 CCATGG C^CATGG
NdeI 3659 CATATG CA^TATG
NsiI 3998 ATGCAT ATGCA^T
NspI 4434 ACATGC [GA]CATG^[CT]
PaeR7I 4813 CTCGAG C^TCGAG
PflMI 971 CCAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3574 AACGTT AA^CGTT
PvuII 2252 CAGCTG CAG^CTG
SnaBI 202 TACGTA TAC^GTA
Van91I 971 CCAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 419 CCATACATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4813 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 4938 GTCTAC GT^[AC][GT]AC
6511 GTATAC
AflII 2871 CTTAAG C^TTAAG
3625 CTTAAG
AflIII 3636 ACACGT A^C[GA][CT]GT
3638 ACGTGT
Asp700I 1853 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2316 GAAACAGTTC
AvaI 4405 CCCGAG C^[CT]CG[GA]G
4813 CTCGAG
BalI 1439 TGGCCA TGG^CCA
4574 TGGCCA
BbrPI 3637 CACGTG CAC^GTG
6129 CACGTG
BclI 3913 TGATCA T^GATCA
5481 TGATCA
BfrI 2871 CTTAAG C^TTAAG
3625 CTTAAG
BsoBI 4405 CCCGAG C^[CT]CG[GA]G
4813 CTCGAG
Bsp1407I 1656 TGTACA T^GTACA
5966 TGTACA
BsrGI 1656 TGTACA T^GTACA
5966 TGTACA
EcoRI 2019 GAATTC G^AATTC
5275 GAATTC
EcoRV 1364 GATATC GAT^ATC
3784 GATATC
FauI 1787 CCCGC CCCGC(4/6)
4523 CCCGC
FbaI 3913 TGATCA T^GATCA
5481 TGATCA
HapII 1678 CCGG C^CGG
4488 CCGG
HincII 2785 GTTGAC GT[CT]^[GA]AC
4012 GTTGAC
HindII 2785 GTTGAC GT[CT]^[GA]AC
4012 GTTGAC
HpaII 1678 CCGG C^CGG
4488 CCGG
MfeI 2073 CAATTG C^AATTG
3064 CAATTG
MluNI 1439 TGGCCA TGG^CCA
4574 TGGCCA
MscI 1439 TGGCCA TGG^CCA
4574 TGGCCA
MspI 1678 CCGG C^CGG
4488 CCGG
MunI 2073 CAATTG C^AATTG
3064 CAATTG
PmaCI 3637 CACGTG CAC^GTG
6129 CACGTG
PmlI 3637 CACGTG CAC^GTG
6129 CACGTG
PpuMI 789 GGGTCCT [GA]G^G[AT]CC[CT]
2339 AGGACCC
SspBI 1656 TGTACA T^GTACA
5966 TGTACA
XmnI 1853 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2316 GAAACAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
AspHI AspI AvaII BanII BbsI
BbvCI BbvI BfaI BglII BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I BspHI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PflFI PleI PshBI PstI
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SpeI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XbaI XhoII