##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Aor51HI AscI AspEI AviII
AvrII BbeI BclI BlnI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI FbaI FseI FspI HgaI
Hin1I HpaI KasI KspI MluI
MroI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 532 GGTACC G^GTACC
AccI 3043 GTCTAC GT^[AC][GT]AC
AflII 4473 CTTAAG C^TTAAG
ApaI 1860 GGGCCC GGGCC^C
AseI 2898 ATTAAT AT^TAAT
AsnI 2898 ATTAAT AT^TAAT
Asp700I 339 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 532 GGTACC G^GTACC
BamHI 1906 GGATCC G^GATCC
BbrPI 4606 CACGTG CAC^GTG
BbsI 4328 GTCTTC GAAGAC(2/6)
BcgI 5854 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5205 GGATAC GTATCC(6/5)
BfrI 4473 CTTAAG C^TTAAG
BglII 5369 AGATCT A^GATCT
BlpI 4511 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4511 GCTTAGC GC^T[ACGT]AGC
BpuAI 4328 GTCTTC GAAGAC(2/6)
BsaAI 4606 CACGTG [CT]AC^GT[GA]
BsaBI 3230 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3990 CGTCTC CGTCTC(1/5)
Bsp1407I 2346 TGTACA T^GTACA
BspHI 2382 TCATGA T^CATGA
BspMI 3291 ACCTGC ACCTGC(4/8)
BsrFI 132 ACCGGC [GA]^CCGG[CT]
BsrGI 2346 TGTACA T^GTACA
CelII 4511 GCTTAGC GC^T[ACGT]AGC
Cfr10I 132 ACCGGC [GA]^CCGG[CT]
DraIII 406 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2487 GACCGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 877 GATATC GAT^ATC
KpnI 532 GGTACC GGTAC^C
MamI 3230 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3605 CAATTG C^AATTG
MslI 1636 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3605 CAATTG C^AATTG
NdeI 2844 CATATG CA^TATG
PflMI 2911 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4606 CACGTG CAC^GTG
PmlI 4606 CACGTG CAC^GTG
PshAI 643 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2898 ATTAAT AT^TAAT
RcaI 2382 TCATGA T^CATGA
SacI 2190 GAGCTC GAGCT^C
SapI 1436 GCTCTTC GCTCTTC(1/4)
SexAI 3148 ACCTGGT A^CC[AT]GGT
SmaI 252 CCCGGG CCC^GGG
SpeI 5459 ACTAGT A^CTAGT
SspBI 2346 TGTACA T^GTACA
Van91I 2911 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 252 CCCGGG C^CCGGG
XmaI 252 CCCGGG C^CCGGG
XmnI 339 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 3601 CGCG CG^CG
5663 CGCG
AflIII 5189 ACATGT A^C[GA][CT]GT
5285 ACATGT
Alw44I 5047 GTGCAC G^TGCAC
5821 GTGCAC
ApaLI 5047 GTGCAC G^TGCAC
5821 GTGCAC
AspI 1733 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2701 GACATAGTC
AvaI 252 CCCGGG C^[CT]CG[GA]G
2688 CCCGAG
BglI 1862 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3865 GCCTAAGGGGC
BsoBI 252 CCCGGG C^[CT]CG[GA]G
2688 CCCGAG
BspLU11I 5189 ACATGT A^CATGT
5285 ACATGT
BstEII 1041 GGTCACC G^GT[ACGT]ACC
1076 GGTCACC
BstPI 1041 GGTCACC G^GT[ACGT]ACC
1076 GGTCACC
BstUI 3601 CGCG CG^CG
5663 CGCG
EcoO65I 1041 GGTCACC G^GT[ACGT]ACC
1076 GGTCACC
EcoT22I 1633 ATGCAT ATGCA^T
4481 ATGCAT
HaeII 3073 GGCGCT [GA]GCGC^[CT]
4128 AGCGCC
HincII 1739 GTCAAC GT[CT]^[GA]AC
2729 GTTGAC
HindII 1739 GTCAAC GT[CT]^[GA]AC
2729 GTTGAC
HindIII 602 AAGCTT A^AGCTT
1554 AAGCTT
MvnI 3601 CGCG CG^CG
5663 CGCG
NsiI 1633 ATGCAT ATGCA^T
4481 ATGCAT
PflFI 1733 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2701 GACATAGTC
ScaI 3299 AGTACT AGT^ACT
4592 AGTACT
Tth111I 1733 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2701 GACATAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XhoII