##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AhdI Aor51HI AscI AseI
AsnI AspEI AviII AvrII BbrPI
BfrI BlnI BsaAI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspEI BspHI
BspLU11I BsrGI Bst1107I BstAPI BstBI
BstZ17I CpoI EagI Eam1105I EclXI
Eco47III Eco52I FseI FspI HindIII
KspI MluI MroI NaeI NgoMIV
NotI NruI NspI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 120 GGTACC G^GTACC
AcyI 3059 GGCGCC G[GA]^CG[CT]C
AgeI 1428 ACCGGT A^CCGGT
Alw44I 4885 GTGCAC G^TGCAC
AocI 1688 CCTGAGG CC^T[ACGT]AGG
ApaI 1820 GGGCCC GGGCC^C
ApaLI 4885 GTGCAC G^TGCAC
Asp718I 120 GGTACC G^GTACC
BbeI 3059 GGCGCC GGCGC^C
BcgI 3819 GCATGAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4112 GGATAC GTATCC(6/5)
BclI 322 TGATCA T^GATCA
BlpI 4040 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4040 GCTGAGC GC^T[ACGT]AGC
BsaBI 323 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3059 GGCGCC G[GA]^CG[CT]C
BspDI 2228 ATCGAT AT^CGAT
BssHII 65 GCGCGC G^CGCGC
Bsu36I 1688 CCTGAGG CC^T[ACGT]AGG
CelII 4040 GCTGAGC GC^T[ACGT]AGC
ClaI 2228 ATCGAT AT^CGAT
DraI 1607 TTTAAA TTT^AAA
DraIII 1738 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1688 CCTGAGG CC^T[ACGT]AGG
EcoRI 3725 GAATTC G^AATTC
EcoT22I 5372 ATGCAT ATGCA^T
FbaI 322 TGATCA T^GATCA
HaeII 3059 GGCGCC [GA]GCGC^[CT]
Hin1I 3059 GGCGCC G[GA]^CG[CT]C
HpaI 1559 GTTAAC GTT^AAC
KasI 3059 GGCGCC G^GCGCC
KpnI 120 GGTACC GGTAC^C
MamI 323 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3508 CAATTG C^AATTG
MunI 3508 CAATTG C^AATTG
NarI 3059 GGCGCC GG^CGCC
NdeI 3530 CATATG CA^TATG
NheI 4951 GCTAGC G^CTAGC
NsiI 5372 ATGCAT ATGCA^T
PinAI 1428 ACCGGT A^CCGGT
ScaI 2455 AGTACT AGT^ACT
SfoI 3059 GGCGCC GGC^GCC
StuI 4809 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspI 758 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4745 GACTTTGTC
AvaI 21 CCCGAG C^[CT]CG[GA]G
3495 CCCGAG
BalI 1659 TGGCCA TGG^CCA
2393 TGGCCA
BamHI 1353 GGATCC G^GATCC
4160 GGATCC
BglI 203 GCCATCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2958 GCCCTTCTGGC
BglII 328 AGATCT A^GATCT
4575 AGATCT
BsoBI 21 CCCGAG C^[CT]CG[GA]G
3495 CCCGAG
BsrBI 1539 CCGCTC CCGCTC(-3/-3)
1059 GAGCGG
BsrFI 1428 ACCGGT [GA]^CCGG[CT]
4096 GCCGGT
BssSI 458 CACGAG CACGAG(-5/-1)
3713 CTCGTG
BstEII 2794 GGTCACC G^GT[ACGT]ACC
3481 GGTCACC
BstPI 2794 GGTCACC G^GT[ACGT]ACC
3481 GGTCACC
Cfr10I 1428 ACCGGT [GA]^CCGG[CT]
4096 GCCGGT
EcoO65I 2794 GGTCACC G^GT[ACGT]ACC
3481 GGTCACC
HgaI 2015 GACGC GACGC(5/10)
158 GCGTC
HincII 1559 GTTAAC GT[CT]^[GA]AC
2537 GTTGAC
HindII 1559 GTTAAC GT[CT]^[GA]AC
2537 GTTGAC
MaeII 2661 ACGT A^CGT
4761 ACGT
MluNI 1659 TGGCCA TGG^CCA
2393 TGGCCA
MscI 1659 TGGCCA TGG^CCA
2393 TGGCCA
PflFI 758 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4745 GACTTTGTC
SapI 1541 GCTCTTC GCTCTTC(1/4)
2803 GCTCTTC
Tth111I 758 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4745 GACTTTGTC
XbaI 1477 TCTAGA T^CTAGA
4696 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoRV EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI