##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccI AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI BcgI BciVI
BclI BsaBI BseAI BsmI BspDI
BspEI BspHI BssSI Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRI
FbaI FseI HindIII HpaI MamI
MluI MroI NdeI NheI NotI
NruI PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 464 GACGTC GACGT^C
AflII 424 CTTAAG C^TTAAG
AspI 656 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3775 TGCGCA TGC^GCA
BamHI 5151 GGATCC G^GATCC
BbeI 193 GGCGCC GGCGC^C
BbrPI 108 CACGTG CAC^GTG
BfrI 424 CTTAAG C^TTAAG
BglI 3066 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 418 AGATCT A^GATCT
BlpI 2945 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2945 GCTGAGC GC^T[ACGT]AGC
BsaI 5341 GAGACC GGTCTC(1/5)
BsaWI 672 ACCGGA [AT]^CCGG[AT]
BsiWI 173 CGTACG C^GTACG
BspLU11I 4837 ACATGT A^CATGT
CelII 2945 GCTGAGC GC^T[ACGT]AGC
EcoT22I 4739 ATGCAT ATGCA^T
FspI 3775 TGCGCA TGC^GCA
HincII 953 GTTGAC GT[CT]^[GA]AC
HindII 953 GTTGAC GT[CT]^[GA]AC
KasI 193 GGCGCC G^GCGCC
KspI 2936 CCGCGG CCGC^GG
MfeI 4210 CAATTG C^AATTG
MunI 4210 CAATTG C^AATTG
NaeI 2439 GCCGGC GCC^GGC
NarI 193 GGCGCC GG^CGCC
NcoI 3379 CCATGG C^CATGG
NgoMIV 2439 GCCGGC G^CCGGC
NsiI 4739 ATGCAT ATGCA^T
PflFI 656 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3379 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 108 CACGTG CAC^GTG
PmlI 108 CACGTG CAC^GTG
PvuII 5000 CAGCTG CAG^CTG
SacI 939 GAGCTC GAGCT^C
SacII 2936 CCGCGG CCGC^GG
SexAI 657 ACCAGGT A^CC[AT]GGT
SfoI 193 GGCGCC GGC^GCC
SmaI 2289 CCCGGG CCC^GGG
SspI 2181 AATATT AAT^ATT
Tth111I 656 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3379 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 512 TCTAGA T^CTAGA
XmaCI 2289 CCCGGG C^CCGGG
XmaI 2289 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 3131 GGTACC G^GTACC
4658 GGTACC
AcyI 193 GGCGCC G[GA]^CG[CT]C
464 GACGTC
AflIII 2083 ACGTGT A^C[GA][CT]GT
4837 ACATGT
Asp700I 395 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3233 GAACCAGTTC
Asp718I 3131 GGTACC G^GTACC
4658 GGTACC
AvrII 199 CCTAGG C^CTAGG
973 CCTAGG
BlnI 199 CCTAGG C^CTAGG
973 CCTAGG
BsaHI 193 GGCGCC G[GA]^CG[CT]C
464 GACGTC
Bsp1407I 3519 TGTACA T^GTACA
4278 TGTACA
BsrDI 4531 CATTGC GCAATG(2/0)
1638 GCAATG
BsrGI 3519 TGTACA T^GTACA
4278 TGTACA
BssHII 126 GCGCGC G^CGCGC
178 GCGCGC
BstBI 1847 TTCGAA TT^CGAA
3240 TTCGAA
EagI 166 CGGCCG C^GGCCG
2437 CGGCCG
EclXI 166 CGGCCG C^GGCCG
2437 CGGCCG
Eco52I 166 CGGCCG C^GGCCG
2437 CGGCCG
EcoRV 360 GATATC GAT^ATC
1166 GATATC
HgaI 227 GACGC GACGC(5/10)
85 GCGTC
Hin1I 193 GGCGCC G[GA]^CG[CT]C
464 GACGTC
KpnI 3131 GGTACC GGTAC^C
4658 GGTACC
NspV 1847 TTCGAA TT^CGAA
3240 TTCGAA
SfuI 1847 TTCGAA TT^CGAA
3240 TTCGAA
SspBI 3519 TGTACA T^GTACA
4278 TGTACA
XmnI 395 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3233 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII