##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AclI AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI AspEI
AspI AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BciVI BcnI
BglI BlnI BsiEI BsiWI BspDI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI FseI HaeII KasI KspI
MluI MluNI MscI NarI NciI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI Sse8387I SwaI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3573 GACGTC GACGT^C
Acc65I 2169 GGTACC G^GTACC
AccI 587 GTCTAC GT^[AC][GT]AC
AccIII 396 TCCGGA T^CCGGA
Asp700I 2366 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2169 GGTACC G^GTACC
AvaI 2852 CCCGAG C^[CT]CG[GA]G
AviII 906 TGCGCA TGC^GCA
BcgI 5386 GCATTTTAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1372 TGATCA T^GATCA
BlpI 3389 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3389 GCTCAGC GC^T[ACGT]AGC
BsaBI 257 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 396 TCCGGA [AT]^CCGG[AT]
BseAI 396 TCCGGA T^CCGGA
BsmBI 724 GAGACG CGTCTC(1/5)
BsoBI 2852 CCCGAG C^[CT]CG[GA]G
BspEI 396 TCCGGA T^CCGGA
BssSI 4446 CTCGTG CACGAG(-5/-1)
CelII 3389 GCTCAGC GC^T[ACGT]AGC
DrdI 785 GACTCTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1372 TGATCA T^GATCA
FspI 906 TGCGCA TGC^GCA
HpaI 5762 GTTAAC GTT^AAC
KpnI 2169 GGTACC GGTAC^C
MamI 257 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 396 TCCGGA T^CCGGA
NaeI 2862 GCCGGC GCC^GGC
NgoMIV 2862 GCCGGC G^CCGGC
NheI 5177 GCTAGC G^CTAGC
PshAI 124 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2542 GAAGAGC GCTCTTC(1/4)
ScaI 4872 AGTACT AGT^ACT
SexAI 3106 ACCAGGT A^CC[AT]GGT
SpeI 289 ACTAGT A^CTAGT
SphI 5011 GCATGC GCATG^C
StuI 664 AGGCCT AGG^CCT
XmnI 2366 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 2174 CTTAAG C^TTAAG
5515 CTTAAG
Alw44I 1465 GTGCAC G^TGCAC
2822 GTGCAC
ApaLI 1465 GTGCAC G^TGCAC
2822 GTGCAC
BfrI 2174 CTTAAG C^TTAAG
5515 CTTAAG
BsgI 2737 CTGCAC GTGCAG(16/14)
2889 CTGCAC
BsrDI 1604 CATTGC GCAATG(2/0)
1429 GCAATG
BsrFI 1106 ACCGGC [GA]^CCGG[CT]
2862 GCCGGC
BstAPI 2829 GCAAATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3329 GCATATGCTGC
BstEII 2843 GGTGACC G^GT[ACGT]ACC
3421 GGTCACC
BstPI 2843 GGTGACC G^GT[ACGT]ACC
3421 GGTCACC
BstXI 966 CCAGAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2980 CCACTGTGATGG
CfoI 907 GCGC GCG^C
3145 GCGC
Cfr10I 1106 ACCGGC [GA]^CCGG[CT]
2862 GCCGGC
DsaI 802 CCATGG C^C[GA][CT]GG
3664 CCATGG
EcoO65I 2843 GGTGACC G^GT[ACGT]ACC
3421 GGTCACC
EcoRV 4348 GATATC GAT^ATC
5274 GATATC
FauI 2436 CCCGC CCCGC(4/6)
740 GCGGG
HhaI 907 GCGC GCG^C
3145 GCGC
HinP1I 907 GCGC G^CGC
3145 GCGC
HindIII 970 AAGCTT A^AGCTT
2162 AAGCTT
MfeI 1645 CAATTG C^AATTG
3901 CAATTG
MunI 1645 CAATTG C^AATTG
3901 CAATTG
NcoI 802 CCATGG C^CATGG
3664 CCATGG
PflMI 1192 CCATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1556 CCACTTGATGG
PstI 2965 CTGCAG CTGCA^G
3136 CTGCAG
SnaBI 385 TACGTA TAC^GTA
4299 TACGTA
TaqI 762 TCGA T^CGA
4720 TCGA
TthHB8I 762 TCGA T^CGA
4720 TCGA
Van91I 1192 CCATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1556 CCACTTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanI BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrBI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HgaI Hin1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XbaI
XhoII