##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccII AcyI AgeI
Aor51HI ApaI AscI Asp718I AspI
AvaI AviII BbeI BbvCI BglI
BsaHI BsiWI BsmBI BsoBI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I CpoI Eco47III FauI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MluI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PvuI RsrII SacII SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 192 TCCGGA T^CCGGA
AclI 652 AACGTT AA^CGTT
AflII 2619 CTTAAG C^TTAAG
AflIII 997 ACATGT A^C[GA][CT]GT
Alw44I 5464 GTGCAC G^TGCAC
ApaLI 5464 GTGCAC G^TGCAC
AvrII 216 CCTAGG C^CTAGG
BamHI 562 GGATCC G^GATCC
BbrPI 4274 CACGTG CAC^GTG
BcgI 4203 CGACATCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1924 GGATAC GTATCC(6/5)
BclI 3352 TGATCA T^GATCA
BfrI 2619 CTTAAG C^TTAAG
BglII 5117 AGATCT A^GATCT
BlnI 216 CCTAGG C^CTAGG
BlpI 3680 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3680 GCTGAGC GC^T[ACGT]AGC
BsaAI 4274 CACGTG [CT]AC^GT[GA]
BsaBI 10 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 192 TCCGGA [AT]^CCGG[AT]
BseAI 192 TCCGGA T^CCGGA
BsiEI 1808 CGGCCG CG[GA][CT]^CG
BsmI 3281 GCATTC GAATGC(1/-1)
BspDI 289 ATCGAT AT^CGAT
BspEI 192 TCCGGA T^CCGGA
BspLU11I 997 ACATGT A^CATGT
BsrBI 4103 GAGCGG CCGCTC(-3/-3)
BsrDI 4240 CATTGC GCAATG(2/0)
BssSI 249 CTCGTG CACGAG(-5/-1)
CelII 3680 GCTGAGC GC^T[ACGT]AGC
CfoI 1872 GCGC GCG^C
ClaI 289 ATCGAT AT^CGAT
DrdI 1879 GACATGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1808 CGGCCG C^GGCCG
EclXI 1808 CGGCCG C^GGCCG
Eco52I 1808 CGGCCG C^GGCCG
EcoNI 4602 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3352 TGATCA T^GATCA
HhaI 1872 GCGC GCG^C
HinP1I 1872 GCGC G^CGC
HindIII 984 AAGCTT A^AGCTT
MamI 10 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3957 CAATTG C^AATTG
MroI 192 TCCGGA T^CCGGA
MunI 3957 CAATTG C^AATTG
NcoI 4592 CCATGG C^CATGG
NdeI 3418 CATATG CA^TATG
NheI 63 GCTAGC G^CTAGC
PmaCI 4274 CACGTG CAC^GTG
PmlI 4274 CACGTG CAC^GTG
Psp1406I 652 AACGTT AA^CGTT
SacI 1438 GAGCTC GAGCT^C
SalI 4201 GTCGAC G^TCGAC
ScaI 2038 AGTACT AGT^ACT
SexAI 2568 ACCTGGT A^CC[AT]GGT
SphI 3310 GCATGC GCATG^C
XbaI 1254 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 595 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3696 GACCGTATGTC
AocI 125 CCTCAGG CC^T[ACGT]AGG
4652 CCTGAGG
Asp700I 2175 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3982 GAAGCACTTC
AspEI 595 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3696 GACCGTATGTC
AspHI 1438 GAGCTC G[AT]GC[AT]^C
5464 GTGCAC
BcnI 1332 CCCGG CC^[GC]GG
3036 CCGGG
BsgI 503 CTGCAC GTGCAG(16/14)
1088 CTGCAC
BsiHKAI 1438 GAGCTC G[AT]GC[AT]^C
5464 GTGCAC
Bsp1407I 3391 TGTACA T^GTACA
4947 TGTACA
BspHI 3293 TCATGA T^CATGA
3563 TCATGA
BspMI 417 ACCTGC ACCTGC(4/8)
694 GCAGGT
BsrFI 615 GCCGGT [GA]^CCGG[CT]
1832 ACCGGC
BsrGI 3391 TGTACA T^GTACA
4947 TGTACA
Bsu36I 125 CCTCAGG CC^T[ACGT]AGG
4652 CCTGAGG
Cfr10I 615 GCCGGT [GA]^CCGG[CT]
1832 ACCGGC
DraIII 2955 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3144 CACATTGTG
DsaI 143 CCACGG C^C[GA][CT]GG
4592 CCATGG
Eam1105I 595 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3696 GACCGTATGTC
Eco81I 125 CCTCAGG CC^T[ACGT]AGG
4652 CCTGAGG
EcoRI 3297 GAATTC G^AATTC
4587 GAATTC
EcoRV 229 GATATC GAT^ATC
1452 GATATC
EcoT22I 3312 ATGCAT ATGCA^T
5506 ATGCAT
NciI 1332 CCCGG CC^[GC]GG
3036 CCGGG
NsiI 3312 ATGCAT ATGCA^T
5506 ATGCAT
RcaI 3293 TCATGA T^CATGA
3563 TCATGA
SspBI 3391 TGTACA T^GTACA
4947 TGTACA
StuI 1543 AGGCCT AGG^CCT
3457 AGGCCT
XmnI 2175 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3982 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AvaII BalI BanI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstEII BstF5I BstNI BstPI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoO65I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII