##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AcyI AgeI Alw44I
ApaLI AscI Asp718I BbeI BcgI
BglI BlpI Bpu1102I BsaHI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraIII EagI EclXI
Eco52I EcoO65I EcoRI FseI HgaI
Hin1I KasI KpnI KspI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PvuI RcaI RsrII SacII
SalI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SspBI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 864 GTAGAC GT^[AC][GT]AC
AccIII 3805 TCCGGA T^CCGGA
AclI 3919 AACGTT AA^CGTT
AflII 2699 CTTAAG C^TTAAG
AhdI 5628 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 104 AGCGCT AGC^GCT
ApaI 4693 GGGCCC GGGCC^C
AspEI 5628 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5044 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1537 CCCGAG C^[CT]CG[GA]G
AvrII 2871 CCTAGG C^CTAGG
BfrI 2699 CTTAAG C^TTAAG
BlnI 2871 CCTAGG C^CTAGG
BsaBI 4649 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3805 TCCGGA [AT]^CCGG[AT]
BseAI 3805 TCCGGA T^CCGGA
BsmFI 531 GGGAC GGGAC(10/14)
BsoBI 1537 CCCGAG C^[CT]CG[GA]G
BspEI 3805 TCCGGA T^CCGGA
BspLU11I 2824 ACATGT A^CATGT
BsrFI 800 GCCGGT [GA]^CCGG[CT]
BstXI 2397 CCAGAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 800 GCCGGT [GA]^CCGG[CT]
DrdI 683 GACTTTAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 5628 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 104 AGCGCT AGC^GCT
HincII 5530 GTTAAC GT[CT]^[GA]AC
HindII 5530 GTTAAC GT[CT]^[GA]AC
HpaI 5530 GTTAAC GTT^AAC
MamI 4649 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 395 ACGCGT A^CGCGT
MroI 3805 TCCGGA T^CCGGA
PflFI 5044 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 533 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3919 AACGTT AA^CGTT
SapI 141 GAAGAGC GCTCTTC(1/4)
SbfI 3362 CCTGCAGG CCTGCA^GG
SmlI 2699 CTTAAG C^T[CT][GA]AG
SpeI 1521 ACTAGT A^CTAGT
Sse8387I 3362 CCTGCAGG CCTGCA^GG
SspI 2263 AATATT AAT^ATT
Tth111I 5044 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 771 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3933 CAGGAGCTG
AocI 2961 CCTCAGG CC^T[ACGT]AGG
4059 CCTGAGG
AseI 4418 ATTAAT AT^TAAT
4620 ATTAAT
AsnI 4418 ATTAAT AT^TAAT
4620 ATTAAT
AviII 586 TGCGCA TGC^GCA
599 TGCGCA
BalI 2528 TGGCCA TGG^CCA
4832 TGGCCA
BamHI 1041 GGATCC G^GATCC
3336 GGATCC
BbvCI 2151 CCTCAGC CCTCAGC(-5/-2)
2606 CCTCAGC
BciVI 4817 GGATAC GTATCC(6/5)
836 GTATCC
BclI 1145 TGATCA T^GATCA
2887 TGATCA
BglII 751 AGATCT A^GATCT
2027 AGATCT
BsaI 3912 GAGACC GGTCTC(1/5)
5594 GGTCTC
Bsu36I 2961 CCTCAGG CC^T[ACGT]AGG
4059 CCTGAGG
Eco81I 2961 CCTCAGG CC^T[ACGT]AGG
4059 CCTGAGG
EcoRV 2433 GATATC GAT^ATC
3711 GATATC
EcoT22I 3255 ATGCAT ATGCA^T
4431 ATGCAT
FauI 609 GCGGG CCCGC(4/6)
697 GCGGG
FbaI 1145 TGATCA T^GATCA
2887 TGATCA
FspI 586 TGCGCA TGC^GCA
599 TGCGCA
HaeII 104 AGCGCT [GA]GCGC^[CT]
670 GGCGCT
MluNI 2528 TGGCCA TGG^CCA
4832 TGGCCA
MscI 2528 TGGCCA TGG^CCA
4832 TGGCCA
NsiI 3255 ATGCAT ATGCA^T
4431 ATGCAT
PpuMI 22 AGGACCC [GA]G^G[AT]CC[CT]
2965 AGGACCT
PshBI 4418 ATTAAT AT^TAAT
4620 ATTAAT
SacI 1945 GAGCTC GAGCT^C
3846 GAGCTC
StuI 3515 AGGCCT AGG^CCT
4772 AGGCCT
XcmI 749 CCAGATCTACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3562 CCATTGCCTGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BanI BanII BbrPI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI