##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AccI        AclI        AflII       AflIII      AgeI        
Alw44I      ApaLI       AscI        AseI        AsnI        
AvrII       BamHI       BciVI       BfrI        BlnI        
BsaBI       BsiWI       BspDI       BspHI       BspLU11I    
BssSI       Bst1107I    BstZ17I     ClaI        CpoI        
DrdI        EagI        EclXI       Eco52I      EcoRI       
EcoRV       EcoT22I     HincII      HindII      HindIII     
HpaI        KspI        MamI        MfeI        MluI        
MunI        NotI        NsiI        PacI        PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SgrAI       SnaBI       
SphI        Sse8387I    SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

Acc65I       5229  GGTACC               G^GTACC
AccIII        414  TCCGGA               T^CCGGA
Aor51HI      5731  AGCGCT               AGC^GCT
Asp700I       751  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      5229  GGTACC               G^GTACC
BclI         5696  TGATCA               T^GATCA
BseAI         414  TCCGGA               T^CCGGA
BsmI         2533  GCATTC               GAATGC(1/-1)
BspEI         414  TCCGGA               T^CCGGA
BssHII        954  GCGCGC               G^CGCGC
BstAPI        889  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3261  GGTCACC              G^GT[ACGT]ACC
BstPI        3261  GGTCACC              G^GT[ACGT]ACC
DraIII        629  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     5731  AGCGCT               AGC^GCT
EcoO65I      3261  GGTCACC              G^GT[ACGT]ACC
FbaI         5696  TGATCA               T^GATCA
KpnI         5229  GGTACC               GGTAC^C
MroI          414  TCCGGA               T^CCGGA
NdeI         6110  CATATG               CA^TATG
NheI         4766  GCTAGC               G^CTAGC
NruI         2803  TCGCGA               TCG^CGA
SapI         3475  GCTCTTC              GCTCTTC(1/4)
SpeI         1780  ACTAGT               A^CTAGT
XmnI          751  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcsI         1289  AAATTC               [GA]^AATT[CT]
             1434  GAATTT              
AhdI          214  GACGCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3254  GACCTCCGGTC         
ApoI         1289  AAATTC               [GA]^AATT[CT]
             1434  GAATTT              
AspEI         214  GACGCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3254  GACCTCCGGTC         
AspI         2207  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3385  GACGCTGTC           
AviII        1842  TGCGCA               TGC^GCA
             4062  TGCGCA              
BbrPI        1700  CACGTG               CAC^GTG
             4641  CACGTG              
BcgI         4537  CGAGGCCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               54  GCAATGAGATCG        
BlpI           34  GCTCAGC              GC^T[ACGT]AGC
             1349  GCTGAGC             
Bpu1102I       34  GCTCAGC              GC^T[ACGT]AGC
             1349  GCTGAGC             
BsaWI         414  TCCGGA               [AT]^CCGG[AT]
             3258  TCCGGT              
BsiEI        2614  CGACCG               CG[GA][CT]^CG
             2891  CGACCG              
Bsp1407I      681  TGTACA               T^GTACA
             5973  TGTACA              
BsrDI          54  GCAATG               GCAATG(2/0)
             1995  GCAATG              
BsrGI         681  TGTACA               T^GTACA
             5973  TGTACA              
BstBI        4427  TTCGAA               TT^CGAA
             5947  TTCGAA              
CelII          34  GCTCAGC              GC^T[ACGT]AGC
             1349  GCTGAGC             
DraI         4723  TTTAAA               TTT^AAA
             5273  TTTAAA              
Eam1105I      214  GACGCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3254  GACCTCCGGTC         
FspI         1842  TGCGCA               TGC^GCA
             4062  TGCGCA              
NspV         4427  TTCGAA               TT^CGAA
             5947  TTCGAA              
PaeR7I       2980  CTCGAG               C^TCGAG
             3704  CTCGAG              
PflFI        2207  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3385  GACGCTGTC           
PmaCI        1700  CACGTG               CAC^GTG
             4641  CACGTG              
PmlI         1700  CACGTG               CAC^GTG
             4641  CACGTG              
SfuI         4427  TTCGAA               TT^CGAA
             5947  TTCGAA              
SspBI         681  TGTACA               T^GTACA
             5973  TGTACA              
StuI         2156  AGGCCT               AGG^CCT
             4318  AGGCCT              
Tth111I      2207  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3385  GACGCTGTC           
XhoI         2980  CTCGAG               C^TCGAG
             3704  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  138 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApaI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BglII       BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
FseI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI