##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Aor51HI ApaI AscI
Asp718I AspI AvaI AviII BanI
BbeI BcgI BclI BcnI BglI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NciI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I Tth111I Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 2826 GTCTAC GT^[AC][GT]AC
AhdI 3089 GACTAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1278 GTGCAC G^TGCAC
AlwNI 4778 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1278 GTGCAC G^TGCAC
AspEI 3089 GACTAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2506 TGGCCA TGG^CCA
BbvCI 1246 CCTCAGC CCTCAGC(-5/-2)
BciVI 4222 GTATCC GTATCC(6/5)
BpmI 1179 CTGGAG CTGGAG(16/14)
BsaI 1158 GGTCTC GGTCTC(1/5)
BseRI 2977 GAGGAG GAGGAG(10/8)
BsmBI 1153 GAGACG CGTCTC(1/5)
BstAPI 1468 GCAATTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1587 CCAATAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 3799 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 4231 CCATGG C^C[GA][CT]GG
EaeI 2506 TGGCCA [CT]^GGCC[GA]
Eam1105I 3089 GACTAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 3799 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 1378 CCCGC CCCGC(4/6)
HapII 3693 CCGG C^CGG
HgaI 2356 GACGC GACGC(5/10)
HindIII 3375 AAGCTT A^AGCTT
HpaII 3693 CCGG C^CGG
MfeI 1882 CAATTG C^AATTG
MluNI 2506 TGGCCA TGG^CCA
MscI 2506 TGGCCA TGG^CCA
MspI 3693 CCGG C^CGG
MunI 1882 CAATTG C^AATTG
NcoI 4231 CCATGG C^CATGG
NdeI 951 CATATG CA^TATG
NheI 4699 GCTAGC G^CTAGC
PpuMI 3799 AGGACCT [GA]G^G[AT]CC[CT]
SacI 1362 GAGCTC GAGCT^C
SapI 3914 GAAGAGC GCTCTTC(1/4)
ScaI 1944 AGTACT AGT^ACT
TaqI 5110 TCGA T^CGA
TthHB8I 5110 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 1379 CCGC CCGC(-3/-1)
2880 GCGG
AflIII 3202 ACGTGT A^C[GA][CT]GT
3270 ACACGT
AvrII 1503 CCTAGG C^CTAGG
3814 CCTAGG
BamHI 93 GGATCC G^GATCC
1371 GGATCC
BanII 1362 GAGCTC G[GA]GC[CT]^C
4087 GGGCTC
BbrPI 3201 CACGTG CAC^GTG
3271 CACGTG
BglII 34 AGATCT A^GATCT
725 AGATCT
BlnI 1503 CCTAGG C^CTAGG
3814 CCTAGG
BsaAI 3201 CACGTG [CT]AC^GT[GA]
3271 CACGTG
BsaBI 1077 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4254 GATTGACATC
Bsp1407I 302 TGTACA T^GTACA
4367 TGTACA
BsrGI 302 TGTACA T^GTACA
4367 TGTACA
BstF5I 98 CATCC GGATG(2/0)
1677 GGATG
EcoNI 4689 CCTTTGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4982 CCTTCCTCAGG
FokI 98 CATCC GGATG(9/13)
1677 GGATG
MamI 1077 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4254 GATTGACATC
NspI 2335 GCATGT [GA]CATG^[CT]
4703 GCATGT
PmaCI 3201 CACGTG CAC^GTG
3271 CACGTG
PmlI 3201 CACGTG CAC^GTG
3271 CACGTG
SpeI 1941 ACTAGT A^CTAGT
4657 ACTAGT
SspBI 302 TGTACA T^GTACA
4367 TGTACA
StuI 3753 AGGCCT AGG^CCT
5313 AGGCCT
SwaI 1063 ATTTAAAT ATTT^AAAT
2896 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AcsI AfaI AflII AluI AlwI
AocI ApoI AseI AsnI Asp700I
AspHI AvaII BbsI BbvI BfaI
BfrI BmyI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstEII
BstNI BstPI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI EarI
Eco81I EcoO65I EcoRI EcoRII EcoRV
EcoT14I EcoT22I Fnu4HI HaeIII HincII
HindII HinfI HpaI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NsiI PleI PshBI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI XhoII XmnI