################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI Alw44I ApaLI AscI Asp718I AviII BamHI BbeI BbrPI BciVI BfrI BsaBI BsaHI BsiEI BsiWI BspDI BspLU11I BssHII BstAPI BstBI BstEII BstPI BstUI ClaI CpoI DrdI EagI EclXI Eco52I EcoO65I FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfoI SfuI SgrAI SmaI SnaBI Sse8387I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccIII 76 TCCGGA T^CCGGA AhdI 3388 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 4668 AGCGCT AGC^GCT ApaI 4533 GGGCCC GGGCC^C AseI 3212 ATTAAT AT^TAAT AsnI 3212 ATTAAT AT^TAAT AspEI 3388 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 498 GTGCTC G[AT]GC[AT]^C AspI 2058 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4748 CCTAGG C^CTAGG BanI 5365 GGCACC G^G[CT][GA]CC BbsI 928 GTCTTC GAAGAC(2/6) BglI 139 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 167 AGATCT A^GATCT BlnI 4748 CCTAGG C^CTAGG BlpI 4245 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4245 GCTCAGC GC^T[ACGT]AGC BpuAI 928 GTCTTC GAAGAC(2/6) BsaAI 4061 TACGTG [CT]AC^GT[GA] BsaWI 76 TCCGGA [AT]^CCGG[AT] BseAI 76 TCCGGA T^CCGGA BsiHKAI 498 GTGCTC G[AT]GC[AT]^C Bsp1407I 2376 TGTACA T^GTACA BspEI 76 TCCGGA T^CCGGA BspMI 4054 GCAGGT ACCTGC(4/8) BsrBI 513 GAGCGG CCGCTC(-3/-3) BsrFI 148 GCCGGT [GA]^CCGG[CT] BsrGI 2376 TGTACA T^GTACA BssSI 99 CACGAG CACGAG(-5/-1) BstXI 2994 CCAGCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 4245 GCTCAGC GC^T[ACGT]AGC Cfr10I 148 GCCGGT [GA]^CCGG[CT] DraIII 4917 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 3388 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4668 AGCGCT AGC^GCT EcoNI 988 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 814 GAATTC G^AATTC EcoRV 639 GATATC GAT^ATC HindIII 5234 AAGCTT A^AGCTT MroI 76 TCCGGA T^CCGGA MspA1I 126 CAGCTG C[AC]G^C[GT]G NheI 876 GCTAGC G^CTAGC PflFI 2058 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 2927 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 4254 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3212 ATTAAT AT^TAAT PvuII 126 CAGCTG CAG^CTG SfiI 138 GGCCACTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 4855 ACTAGT A^CTAGT SspBI 2376 TGTACA T^GTACA Tth111I 2058 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AflIII 567 ACACGT A^C[GA][CT]GT 4062 ACGTGT AocI 855 CCTGAGG CC^T[ACGT]AGG 5276 CCTGAGG AvaI 3033 CCCGAG C^[CT]CG[GA]G 4948 CTCGGG BcgI 101 CGAGGCTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1973 GCAGGAGAATCG BclI 2479 TGATCA T^GATCA 3284 TGATCA BsmBI 1896 CGTCTC CGTCTC(1/5) 5322 CGTCTC BsoBI 3033 CCCGAG C^[CT]CG[GA]G 4948 CTCGGG Bsu36I 855 CCTGAGG CC^T[ACGT]AGG 5276 CCTGAGG Eco81I 855 CCTGAGG CC^T[ACGT]AGG 5276 CCTGAGG FbaI 2479 TGATCA T^GATCA 3284 TGATCA SmlI 2699 CTTGAG C^T[CT][GA]AG 4896 CTTGAG StuI 996 AGGCCT AGG^CCT 4492 AGGCCT SwaI 2742 ATTTAAAT ATTT^AAAT 4030 ATTTAAAT TaqI 4852 TCGA T^CGA 5291 TCGA TthHB8I 4852 TCGA T^CGA 5291 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaII BalI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrDI BsrI BssKI Bst1107I BstF5I BstNI BstYI BstZ17I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NsiI NspI PleI PstI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SphI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI