##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflII       AgeI        Alw44I      ApaLI       AscI        
Asp718I     AviII       BamHI       BbeI        BbrPI       
BciVI       BfrI        BsaBI       BsaHI       BsiEI       
BsiWI       BspDI       BspLU11I    BssHII      BstAPI      
BstBI       BstEII      BstPI       BstUI       ClaI        
CpoI        DrdI        EagI        EclXI       Eco52I      
EcoO65I     FseI        FspI        HgaI        Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
MvnI        NaeI        NarI        NgoMIV      NotI        
NruI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfoI        SfuI        
SgrAI       SmaI        SnaBI       Sse8387I    Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII         76  TCCGGA               T^CCGGA
AhdI         3388  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      4668  AGCGCT               AGC^GCT
ApaI         4533  GGGCCC               GGGCC^C
AseI         3212  ATTAAT               AT^TAAT
AsnI         3212  ATTAAT               AT^TAAT
AspEI        3388  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         498  GTGCTC               G[AT]GC[AT]^C
AspI         2058  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        4748  CCTAGG               C^CTAGG
BanI         5365  GGCACC               G^G[CT][GA]CC
BbsI          928  GTCTTC               GAAGAC(2/6)
BglI          139  GCCACTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         167  AGATCT               A^GATCT
BlnI         4748  CCTAGG               C^CTAGG
BlpI         4245  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     4245  GCTCAGC              GC^T[ACGT]AGC
BpuAI         928  GTCTTC               GAAGAC(2/6)
BsaAI        4061  TACGTG               [CT]AC^GT[GA]
BsaWI          76  TCCGGA               [AT]^CCGG[AT]
BseAI          76  TCCGGA               T^CCGGA
BsiHKAI       498  GTGCTC               G[AT]GC[AT]^C
Bsp1407I     2376  TGTACA               T^GTACA
BspEI          76  TCCGGA               T^CCGGA
BspMI        4054  GCAGGT               ACCTGC(4/8)
BsrBI         513  GAGCGG               CCGCTC(-3/-3)
BsrFI         148  GCCGGT               [GA]^CCGG[CT]
BsrGI        2376  TGTACA               T^GTACA
BssSI          99  CACGAG               CACGAG(-5/-1)
BstXI        2994  CCAGCTACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        4245  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        148  GCCGGT               [GA]^CCGG[CT]
DraIII       4917  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     3388  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     4668  AGCGCT               AGC^GCT
EcoNI         988  CCTTGAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         814  GAATTC               G^AATTC
EcoRV         639  GATATC               GAT^ATC
HindIII      5234  AAGCTT               A^AGCTT
MroI           76  TCCGGA               T^CCGGA
MspA1I        126  CAGCTG               C[AC]G^C[GT]G
NheI          876  GCTAGC               G^CTAGC
PflFI        2058  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        2927  AGGTCCC              [GA]G^G[AT]CC[CT]
PshAI        4254  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3212  ATTAAT               AT^TAAT
PvuII         126  CAGCTG               CAG^CTG
SfiI          138  GGCCACTCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         4855  ACTAGT               A^CTAGT
SspBI        2376  TGTACA               T^GTACA
Tth111I      2058  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AflIII        567  ACACGT               A^C[GA][CT]GT
             4062  ACGTGT              
AocI          855  CCTGAGG              CC^T[ACGT]AGG
             5276  CCTGAGG             
AvaI         3033  CCCGAG               C^[CT]CG[GA]G
             4948  CTCGGG              
BcgI          101  CGAGGCTTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1973  GCAGGAGAATCG        
BclI         2479  TGATCA               T^GATCA
             3284  TGATCA              
BsmBI        1896  CGTCTC               CGTCTC(1/5)
             5322  CGTCTC              
BsoBI        3033  CCCGAG               C^[CT]CG[GA]G
             4948  CTCGGG              
Bsu36I        855  CCTGAGG              CC^T[ACGT]AGG
             5276  CCTGAGG             
Eco81I        855  CCTGAGG              CC^T[ACGT]AGG
             5276  CCTGAGG             
FbaI         2479  TGATCA               T^GATCA
             3284  TGATCA              
SmlI         2699  CTTGAG               C^T[CT][GA]AG
             4896  CTTGAG              
StuI          996  AGGCCT               AGG^CCT
             4492  AGGCCT              
SwaI         2742  ATTTAAAT             ATTT^AAAT
             4030  ATTTAAAT            
TaqI         4852  TCGA                 T^CGA
             5291  TCGA                
TthHB8I      4852  TCGA                 T^CGA
             5291  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AvaII       
BalI        BanII       BbvCI       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspHI       BsrDI       
BsrI        BssKI       Bst1107I    BstF5I      BstNI       
BstYI       BstZ17I     Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
EcoT22I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspI        MvaI        MwoI        NciI        NcoI        
NdeI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PstI        RcaI        RsaI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SphI        SspI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XhoII       XmnI